Virus-Induced Gene Silencing in Nepeta

Palmer, Lira; O’Connor, Sarah E.

doi:10.1007/978-1-0716-0751-0_9

Cited by 3 publications

(1 citation statement)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A recent report showed that TRV-VIGS can be used as a rapid gene function test for the complementation of a loss-of-function mutation in Antirrhinum majus L. (Tan et al, 2020). VIGS technology has also been established and adapted to target genes involved in the production of nepetalactone in Nepeta cataria (catnip) and Nepeta mussinii (catmint) of the Lamiaceae family (mint) (Palmer and O'Connor, 2020). A robust protocol for VIGS of sweet basil (Ocimum basilicum) ObChlH has recently been developed by vacuum infiltration with pTRV constructs (Misra et al, 2020).…”

Section: Other Commercial and Medicinal Plantsmentioning

confidence: 99%

A Methodological Advance of Tobacco Rattle Virus-Induced Gene Silencing for Functional Genomics in Plants

et al. 2021

View full text Add to dashboard Cite

As a promising high-throughput reverse genetic tool in plants, virus-induced gene silencing (VIGS) has already begun to fulfill some of this promise in diverse aspects. However, review of the technological advancements about widely used VIGS system, tobacco rattle virus (TRV)-mediated gene silencing, needs timely updates. Hence, this article mainly reviews viral vector construction, inoculation method advances, important influential factors, and summarizes the recent applications in diverse plant species, thus providing a better understanding and advice for functional gene analysis related to crop improvements.

show abstract

Section: Other Commercial and Medicinal Plantsmentioning

confidence: 99%

A Methodological Advance of Tobacco Rattle Virus-Induced Gene Silencing for Functional Genomics in Plants

et al. 2021

View full text Add to dashboard Cite

show abstract

In vivo characterization of key iridoid biosynthesis pathway genes in catnip (Nepeta cataria)

Palmer

Chuang

Siegmund

et al. 2022

Planta

View full text Add to dashboard Cite

Main conclusion Using virus-induced gene silencing, we demonstrated that the enzymes GES, ISY, and MLPL are responsible for nepetalactone biosynthesis in Nepeta cataria. Abstract Nepetalactone is the main iridoid that is found in the Nepeta genus and is well-known for its psychoactive effect on house cats. Moreover, there is a burgeoning interest into the effect of nepetalactone on insects. Although the enzymes for nepetalactone biosynthesis have been biochemically assayed in vitro, validation of the role that these enzymes have in planta has not been demonstrated. Virus-induced gene silencing (VIGS) is a silencing method that relies on transient transformation and is an approach that has been particularly successful when applied to a variety of non-model plants. Here, we use a recently designed visual-marker dependent VIGS system to demonstrate that the nepetalactone biosynthetic enzymes GES, ISY, and MLPL impact nepetalactone biosynthesis in Nepeta cataria.

show abstract

Optimization of Virus-induced Phytoene Desaturase (PDS) gene silencing using Tobacco Rattle Virus in Coleus forskohlii

Bhat,

Sharma,

Gandhi

2024

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

Virus-Induced Gene Silencing in Nepeta

Cited by 3 publications

References 14 publications

A Methodological Advance of Tobacco Rattle Virus-Induced Gene Silencing for Functional Genomics in Plants

A Methodological Advance of Tobacco Rattle Virus-Induced Gene Silencing for Functional Genomics in Plants

In vivo characterization of key iridoid biosynthesis pathway genes in catnip (Nepeta cataria)

Optimization of Virus-induced Phytoene Desaturase (PDS) gene silencing using Tobacco Rattle Virus in Coleus forskohlii

Contact Info

Product

Resources

About