Virulence, resistance, and genetic relatedness of Escherichia coli and Klebsiella sp. isolated from mule foals

Carneiro, Vanessa Couto; Lessa, Daniel Augusto Barroso; Guttmann, Paul; Magalhães, Helena; Aquino, Maria Helena Cosendey de; Cunha, Luís Eduardo Ribeiro da; Arais, Lavicie Rodrigues; Cerqueira, Aloysio de Mello Figueiredo

doi:10.1590/1678-4162-9115

Cited by 6 publications

(6 citation statements)

References 24 publications

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“…The virulence factors associated with K pneumoniae were determined by targeting specific gene fimH encoding for type 1 fimbriae, the main virulence factor of the bacteria, using primer F 0 -ATGAACGCCTGGTCCTTTGC, R-GCT GAACGCCTATCCCCTGC, 26 and EntB gene responsible for enterobactin production using primer F-ATTTCC TCAACTTCTGGGGC and R-AGCATCGGTGGCGGT GGTCA. 27…”

Section: Molecular Detection Of Virulence Factorsmentioning

confidence: 99%

Virulent and multidrug‐resistant Klebsiella pneumoniae from clinical samples in Balochistan

Fatima

Liaqat

Akbar

et al. 2021

International Wound Journal

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Klebsiella pneumoniae is an important pathogen causing hospital-acquired infections in human beings. Samples from suspected patients of K pneumoniae associated with respiratory and urinary tract infections were collected at Bolan Medical Complex, Quetta, Balochistan. Clinical samples (n = 107) of urine and sputum were collected and processed for K pneumoniae isolation using selective culture media. Initially, 30 of 107 isolates resembling Klebsiella spp. were processed for biochemical profiling and molecular detection using gyrase A (gyrA) gene for conformation. The K pneumoniae isolates were analysed for the presence of drug resistance and virulence genes in their genomes. The 21 of 107 (19.6%) isolates were finally confirmed as K pneumoniae pathogens. An antibiogram study conducted against 17 different antibiotics showed that a majority of the isolates are multidrug resistant. All the isolates (100%) were resistant to amoxicillin, cefixime, amoxicillin-clavulanic acid, cefotaxime, and ceftriaxone followed by tetracycline (95.2%), ciprofloxacin and gentamicin (76.2%), sulphamethoxazol (66.7%), nalidixic acid (61.9%), norfloxacine (42.9%), piperacillin-tazobactam (23.8%), cefoperazone-sulbactam (19%), and cefotaxime-clavulanic acid (33.3%), whereas all the isolates showed sensitivity to amikacin, chloramphenicol, and imipenem. The presence of tetracycline, sulphamethoxazol-resistant genes, and extended-spectrum beta-lactamase was reconfirmed using different specific genes. The presence of virulence genes fimH1 and EntB responsible for adherence and enterobactin production was confirmed in the isolates. The high virulence and drug resistance potential of these Klebsiella isolates are of high public health concern. Multidrug resistance and virulence potential in K. pneumoniae are converting these nosocomial pathogens into superbugs and making its management harder.Sareeen Fatima and Faiza Liaqat contributed equally to the scientific work.

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Section: Molecular Detection Of Virulence Factorsmentioning

confidence: 99%

Virulent and multidrug‐resistant Klebsiella pneumoniae from clinical samples in Balochistan

Fatima

Liaqat

Akbar

et al. 2021

International Wound Journal

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“…Virulence genes characteristic of E. coli were also detected in non– E. coli strains. Carneiro et al (Carneiro et al 2017 ) observed the presence of fim H, pap C, and hly D virulence genes, which are usually detected in E. coli, also in K. pneumoniae strains from fecal samples. However, the prevalence of most virulence genes differed between non– E. coli and E. coli strains.…”

Section: Resultsmentioning

confidence: 99%

The challenges in the identification of Escherichia coli from environmental samples and their genetic characterization

Osińska

Korzeniewska

Korzeniowska‐Kowal

et al. 2022

Environ Sci Pollut Res

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Escherichia coli bacteria are an essential indicator in evaluations of environmental pollution, which is why they must be correctly identified. This study aimed to determine the applicability of various methods for identifying E. coli strains in environmental samples. Bacterial strains preliminary selected on mFc and Chromocult media as E. coli were identified using MALDI Biotyper techniques, based on the presence of genes characteristic of E. coli (uidA, uspA, yaiO), as well as by 16S rRNA gene sequencing. The virulence and antibiotic resistance genes pattern of bacterial strains were also analyzed to investigate the prevalence of factors that may indicate adaptation to unsupportive environmental conditions and could have any significance in further identification of E. coli. Of the strains that had been initially identified as E. coli with culture-based methods, 36–81% were classified as E. coli with the use of selected techniques. The value of Cohen’s kappa revealed the highest degree of agreement between the results of 16S rRNA gene sequencing, the results obtained in the MALDI Biotyper system, and the results of the analysis based on the presence of the yaiO gene. The results of this study could help in the selection of more accurate and reliable methods which can be used in a preliminary screening and more precise identification of E. coli isolated from environmental samples.

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“…Furthermore, Carneiro et al . [ 46 ] reported that Klebsiella isolates were detected in 14.1% of 56 mule foals, primarily ampicillin-resistant, MDR, and ESBL producers. The bla SHV gene was more frequently found in K. pneumoniae isolates in Brazil.…”

Section: Discussionmentioning

confidence: 99%

Determination of extended-spectrum β-lactamase-producing Klebsiella pneumoniae isolated from horses with respiratory manifestation

et al. 2022

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Background and Aim: The World Health Organization considers multidrug-resistant (MDR) Klebsiella pneumoniae a major global threat. Horses harbor commensal isolates of this bacterial species and potentially serve as reservoirs for human MDR bacteria. This study investigated antimicrobial resistance in horses caused by extended-spectrum β-lactamase (ESBL)-producing K. pneumoniae. Materials and Methods: One hundred fifty-nine nasal swab samples were collected from horses with respiratory distress not treated with cefotaxime and erythromycin. Biochemical and serological identification was performed on all samples. Polymerase chain reaction (PCR) was used to detect 16S-23S ITS, mucoviscosity-associated gene (magA), uridine diphosphate galacturonate 4-epimerase gene (uge), and iron uptake system gene (kfu), blaTEM, blaSHV, and blaCTX genes. Sequence analysis and phylogenetic relatedness of randomly selected K. pneumoniae isolates carrying the blaTEM gene were performed. Results: Ten isolates of Klebsiella spp. were obtained from 159 samples, with an incidence of 6.28% (10 of 159). Based on biochemical and serological identification, K. pneumoniae was detected in 4.4% (7 of 159) of the samples. Using PCR, all tested K. pneumoniae isolates (n=7) carried the 16S-23S ITS gene. By contrast, no isolates carried magA, uge, and kfu genes. The blaTEM gene was detected in all test isolates. Moreover, all isolates did not harbor the blaSHV or blaCTX gene. Sequence analysis and phylogenetic relatedness reported that the maximum likelihood unrooted tree generated indicated the clustering of the test isolate with the other Gram-negative isolate blaTEM. Finally, the sequence distance of the blaTEM gene of the test isolate (generated by Lasergene) showed an identity range of 98.4-100% with the blaTEM gene of the different test isolates. Conclusion: The misuse of antimicrobials and insufficient veterinary services might help generate a population of ESBL-producing K. pneumoniae in equines and humans, representing a public health risk.

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Virulence, resistance, and genetic relatedness of Escherichia coli and Klebsiella sp. isolated from mule foals

Cited by 6 publications

References 24 publications

Virulent and multidrug‐resistant Klebsiella pneumoniae from clinical samples in Balochistan

Virulent and multidrug‐resistant Klebsiella pneumoniae from clinical samples in Balochistan

The challenges in the identification of Escherichia coli from environmental samples and their genetic characterization

Determination of extended-spectrum β-lactamase-producing Klebsiella pneumoniae isolated from horses with respiratory manifestation

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