Virulence of<i>Mycobacterium tuberculosis</i>CDC1551 and H37Rv in Rabbits Evaluated by Lurie’s Pulmonary Tubercle Count Method

Bishai, William R.; Dannenberg, Arthur M.; Parrish, Nikki; Ruíz, Rafael; Chen, Ping; Zook, Bernard C.; Johnson, Walter; Boles, James W.; Pitt, Michelle

doi:10.1128/iai.67.9.4931-4934.1999

Cited by 78 publications

(19 citation statements)

References 22 publications

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“…These strains belong to the Euro‐American lineage (principal genetic group 2, cluster IV). For this reason, strain CDC1551, which has been extensively characterized in terms of its biology 47–51 and belongs to the same group but different cluster ( i.e . cluster V) was included in this study as a reference for S75 strains.…”

Section: Discussionmentioning

confidence: 99%

Descriptive proteomic analysis shows protein variability between closely related clinical isolates of Mycobacterium tuberculosis

et al. 2010

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The use of isobaric tags such as iTRAQ allows the relative and absolute quantification of hundreds of proteins in a single experiment for up to eight different samples. More classical techniques such as 2-DE can offer a complimentary approach for the analysis of complex protein samples. In this study, the proteomes of secreted and cytosolic proteins of genetically closely related strains of Mycobacterium tuberculosis were analyzed. Analysis of 2-D gels afforded 28 spots with variations in protein abundance between strains. These were identified by tandem MS/MS. Meanwhile, a rigorous statistical analysis of iTRAQ data allowed the identification and quantification of 101 and 137 proteins in the secreted and cytosolic fractions respectively. Interestingly, several differences in protein levels were observed between the closely related strains BE, C28 and H6. Seven proteins related to cell wall and cell processes were more abundant in BE, while enzymes related to metabolic pathways (GltA2, SucC, Gnd1, Eno) presented lower levels in the BE strain. Proteins involved in iron and sulfur acquisition (BfrB, ViuB, TB15.3 and SseC2) were more abundant in C28 and H6. In general, iTRAQ afforded rapid identification of fine differences between protein levels such as those presented between closely related strains. This provides a platform from which the relevance of these differences can be assessed further using complimentary proteomic and biological modeling methods.

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Section: Discussionmentioning

confidence: 99%

Descriptive proteomic analysis shows protein variability between closely related clinical isolates of Mycobacterium tuberculosis

et al. 2010

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“…However, rabbits are still an excellent animal model for human TB vaccine research because of the similar manifestations of lesions (granulomas, liquefaction, and cavities) to those observed in humans [14,15]. In particular, rabbit models have been extensively used to screen and evaluate potential vaccine candidates (such as BCG, M. vaccae, M. microti and subunit vaccines), and to determine the pathogenic factors and pathogenesis of cavities induced by M. tuberculosis H37Rv infection [15][16][17][18][19][20]83]. In addition, Tsenova et al [84] reported large confluent granulomas with expansive areas of central necrosis in the lungs of rabbits infected with M. tuberculosis HN878 strain.…”

Section: Rabbitsmentioning

confidence: 99%

Animal Models of Tuberculosis Vaccine Research: An Important Component in the Fight against Tuberculosis

Gong

Liang

2020

BioMed Research International

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Tuberculosis (TB), an infectious disease caused by Mycobacterium tuberculosis, is one of the top ten infectious diseases worldwide, and is the leading cause of morbidity from a single infectious agent. M. tuberculosis can cause infection in several species of animals in addition to humans as the natural hosts. Although animal models of TB disease cannot completely simulate the occurrence and development of human TB, they play an important role in studying the pathogenesis, immune responses, and pathological changes as well as for vaccine research. This review summarizes the commonly employed animal models, including mouse, guinea pig, rabbit, rat, goat, cattle, and nonhuman primates, and their characteristics as used in TB vaccine research, and provides a basis for selecting appropriate animal models according to specific research needs. Furthermore, some of the newest animal models used for TB vaccine research (such as humanized animal models, zebrafish, Drosophila, and amoeba) are introduced, and their characteristics and research progress are discussed.

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“…Because CDC1551 induced vigorous TST reactions despite occasionally minor exposure, it was initially considered to be a virulent strain. Further analysis revealed CDC1551 to be of no greater virulence in animals than other strains in terms of its growth in vitro and in vivo (30). However, it was found to induce a marked proinflammatory cytokine response in mononuclear phagocytes: a mechanism proposed to underlie the marked TST responses that were observed (17).…”

Section: Discussionmentioning

confidence: 99%

Transmission ofMycobacterium tuberculosisUndetected by Tuberculin Skin Testing

Anderson¹,

Williams²,

Brown³

et al. 2006

Am J Respir Crit Care Med

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Virulence ofMycobacterium tuberculosisCDC1551 and H37Rv in Rabbits Evaluated by Lurie’s Pulmonary Tubercle Count Method

Cited by 78 publications

References 22 publications

Descriptive proteomic analysis shows protein variability between closely related clinical isolates of Mycobacterium tuberculosis

Descriptive proteomic analysis shows protein variability between closely related clinical isolates of Mycobacterium tuberculosis

Animal Models of Tuberculosis Vaccine Research: An Important Component in the Fight against Tuberculosis

Transmission ofMycobacterium tuberculosisUndetected by Tuberculin Skin Testing

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