Virulence in Mice of Colonial Variants of Candida albicans

Eisman, P. C.; Geftic, Sam G.; Mayer, Rudolf

doi:10.3181/00379727-82-20086

Cited by 22 publications

(7 citation statements)

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“…Stock cultures of all three strains were maintained on Sabouraud Dextrose Agar (Difco) slants at 23 C. Cultures were transferred to new slants every 2 weeks. Large batches of cells were cultured in a medium which contained the followingper liter: KH2PO4, 10g; K2HPO4,5 g; (NH4)2SO4, 2 g; trisodium citrate, 1.0 g; MgCl2-6H20, 0.1 g; MnSO4-7H20, 0.1 g; CaCl2, 0.1 g; ZnSO4.7H20, 0.1 g; biotin, 5,ug; glucose (sterilized separately), 15 g. Methionine, methylmethionine, and homocysteine (concentration, 5 ,umoles/ml) were added to the above medium to stimulate the production of S-adenosylmethionine (19). A medium containing the latter supplements will be referred to below as "supplemented medium."…”

Section: Methodsmentioning

confidence: 99%

Ultraviolet Microscopy ofCandida albicans

Balish

Svihla

1966

J Bacteriol

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1966.-Yeast and mycelial strains of Candida albicans were grown in medium supplemented with sulfur amino acids in an effort to determine factors that control the morphology and pathogenicity of the organism. Ultraviolet microscopy revealed a greater concentration of S-adenosylmethionine in the vacuoles of the mycelial phase than in those of yeast phases. Supplementation with amino acids greatly increased the concentration of S-adenosylmethionine in the mycelial phase, and made these cells more sensitive to the lytic action of snail gut enzymes than two yeast phase strains. This indicates a difference in cell wall structure that may be related to the pathogenicity of the mycelial phase. use were first cultured on slants of Sabouraud Dextrose Agar for 18 hr at 30 C. From the slants, the cultures were transferred to 125-ml Erlenmeyer flasks that contained 20 ml of the medium described above.

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Section: Methodsmentioning

confidence: 99%

Ultraviolet Microscopy ofCandida albicans

Balish

Svihla

1966

J Bacteriol

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“…1 Eisman et al first published in vitro and in vivo data on the anti-tuberculosis activity of substituted thioureas tested in mice and guinea pigs. 2 Subsequent modification and biological testing produced a library of compounds, in which the thiourea derivative 4,4 0 -diisoamythio-carbanilide (isoxyl, thiocarlide, ISO [1]) was shown to have considerable antimycobacterial activity in mice, guinea pigs and rabbits. 3,4 Clinical use of ISO began in the 1960s to treat tuberculosis.…”

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Symmetrical and unsymmetrical analogues of isoxyl; active agents against Mycobacterium tuberculosis

Bhowruth

Brown

Reynolds

et al. 2006

Bioorganic & Medicinal Chemistry Letters

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“…First, there is the longheld view that dimorphism is somehow related to virulence of the organism. However, conflicting evidence implicating the yeast cell (3,23), the mycelial form (7, 25) or both (12, 21) as the virulent entity has confused rather than clarified the situation. Hence, it is impossible at present to give a definite statement concerning this matter.…”

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Changes in cyclic nucleotide levels and dimorphic transition in Candida albicans

Niimi¹,

Niimi²,

Tokunaga³

et al. 1980

J Bacteriol

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The relationship between the levels of cyclic nucleotides and dimorphic transition in Candida albicans was examined. The results showed that cells of this pathogenic fungus contained both cyclic adenosine 3',5'-monophosphate (cAMP) and cyclic guanosine 3',5'-monophosphate (cGMP), the concentration of the latter being about one-tenth that of the former in stationary-phase cells of the yeast form. Our results further indicated that germ tube formation induced by incubation at 40 degrees C followed a rise in cAMP concentration in the cell with no accompanying change in cGMP content. Cysteine, which suppressed germination, also reversed the increase in intracellular cAMP concentration. Dibutyryl cAMP (1 MM) significantly promoted germination in proline medium at temperatures of 32 to 34 degrees C. These results suggested that cAMP was one of the controlling factors in the morphological transition in Candida albicans.

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Virulence in Mice of Colonial Variants of Candida albicans

Cited by 22 publications

References 0 publications

Ultraviolet Microscopy ofCandida albicans

Ultraviolet Microscopy ofCandida albicans

Symmetrical and unsymmetrical analogues of isoxyl; active agents against Mycobacterium tuberculosis

Changes in cyclic nucleotide levels and dimorphic transition in Candida albicans

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