Virulence factors in Escherichia coli strains isolated from urinary tract infection and pyometra cases and from feces of healthy dogs

Siqueira, Amanda Keller; Ribeiro, Márcio Garcia; Leite, Domingos da Silva; Tiba, Monique Ribeiro; Moura, Cláudia de; Lopes, Maria Denise; Prestes, Nereu Carlos; Salerno, Tatiana; Silva, Aristeu Vieira da

doi:10.1016/j.rvsc.2008.07.018

Cited by 74 publications

(74 citation statements)

References 29 publications

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“…Escherichia coli are a very diverse species of bacteria found naturally in humans and animals in terms of gene content, phenotype, and virulence [1]. The fluoroquinolones are the most commonly used class of antibacterials in the treatment of several infectious caused by E. coli, although effective treatment of E. coli infections is impaired by the swift emergence of fluoroquinolone resistance.…”

Section: Introductionmentioning

confidence: 99%

Association between virulence profile and fluoroquinolone resistance in Escherichia coli isolated from dogs and cats in China

Liu

et al. 2017

J Infect Dev Ctries

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Introduction: Escherichia coli is not only a commensal organism in humans and animals, but also a causative agent of diarrhea and extraintestinal infections. Information about the relationship between population structure, virulence gene profiles, and fluoroquinolone resistance of E. coli in dogs and cats in China is limited. Methodology: A total of 174 pathogenic and commensal E. coli isolates were evaluated in terms of phylogenetic group, virulence gene profile, sequence types (STs), and fluoroquinolone susceptibility. Results: A total of 46.6% of isolates belonged to phylogenetic group B2. Isolates displayed high resistance to tetracycline (82.2%), amoxicillin/clavulanic acid (73.6%), gentamicin (62.1%), and enrofloxacin (60.9%). fimH (81.6%) was the most prevalent virulence gene, and 83.9% of isolates contained one or more investigated virulence genes. The majority of the investigated virulence genes were more prevalent in fluoroquinolone-susceptible isolates and pathogenic isolates. Multilocus sequence typing (MLST) showed that E. coli isolates analyzed were assigned to 65 STs. Among of them, pathogenic-resistant and pathogenic-susceptible isolates had 44 and 10 STs, respectively, while there were 8 and 3 STs in the commensal resistant and susceptible isolates, respectively. Conclusions: Phylogenetic group B2 was the dominant group, accounting for 46.6% of the isolates. Pathogenic isolates and fluoroquinolonesusceptible isolates possessed more virulence genes. Pathogenic isolates and fluoroquinolone-resistant isolates exhibited high population diversity, and pandemic clone ST131 appeared in 9.8% of isolates.

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Section: Introductionmentioning

confidence: 99%

Association between virulence profile and fluoroquinolone resistance in Escherichia coli isolated from dogs and cats in China

Liu

et al. 2017

J Infect Dev Ctries

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“…Isolation of diarrhoeagenic antimicrobial-resistant E. coli from dogs with or without diarrhoea and/or their handlers have been reported in countries such as Italy (Carattoli et al 2005), Portugal (Costa et al 2008, Bien et al 2011, Poland (Rzewuska et al 2015), Brazil (de Almeida et al 2012, Paula and Marin 2008, Paula and Marin 2009, Siqueira et al 2009, Puno-Sarmiento et al 2013, the Netherlands (Ewers et al 2010, Ewers et al 2012, Argentina (Bentancor et al 2007), America (Shaheen et al 2011), and Egypt (Ali andMetwaly 2015, Yunis et al 2015). In the available literature, studies on pathogenic E. coli in diarrhoeic and/or healthy dogs in Iran include the reports of Zahrei Salehi et al (2011) and Koochakzadeh et al (2014).…”

Section: Introductionmentioning

confidence: 99%

Detection of virulence and antimicrobial resistance genes in Escherichia coli isolates from diarrhoiec dogs in Iran

Torkan¹,

Bahadoranian²,

Khamesipour³

et al. 2016

Arch. med. vet.

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“…E. coli strains associated with canine pyometra are clonally related to E. coli strains that inhabit in the canine intestine (Wadas et al 1996). Besides this, uropathogenic E. coli (UPEC) differs from commensal intestinal E. coli due to presence of specialized virulence factors (Siqueira et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Phylo-typing of clinicalEscherichia coliisolates originating from bovine mastitis and canine pyometra and urinary tract infection by means of quadruplex PCR

Müştak

Günaydin²,

Kaya

et al. 2015

Veterinary Quarterly

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(2015) Phylo-typing of clinical Escherichiacoli isolates originating from bovine mastitis and canine pyometra and urinary tract infection by means of quadruplex PCR, Veterinary Quarterly, 35:4, 194-199, DOI: 10.1080/01652176.2015 Background: Escherichia coli is one of the major causative agents of bovine mastitis worldwide, and is typically associated with acute, clinical mastitis. Besides this, E. coli strains which belong to the extra-intestinal pathogenic group are also the major cause of urinary tract infections and pyometra in dogs. Objectives: In this study, it was aimed to investigate phylo-groups/subgroups in 155 E. coli isolates obtained from acute bovine mastitis, 43 from urinary tract infections of dogs and 20 from canine pyometra by a formerly described triplex PCR and recently described new quadruplex polymerase chain reaction (PCR) method. Results: Group A 1 (n D 118; 76%) and B1 (n D 71; 46%) were found to be the most prevalent groups by triplex and quadruplex PCR assays in mastitis isolates, respectively. Phylo-typing of 43 urinary tract isolates also revealed that most of the isolates belonged to A 1 (n D 23; 54%) by triplex and B2 (n D 36; 84%) by quadruplex PCR assays. The isolates assigned as group A 1 (n D 17; 85%) by triplex PCR could not be classified by quadruplex PCR in pyometra isolates. Conclusions:The results support the hypothesis that E. coli strains isolated from bovine mastitis cases are environmental. Also, groups C, E and F were identified as new phylo-groups for the first time in acute bovine mastitis cases. The comparison of triplex PCR with quadruplex PCR results revealed that most of the groups assigned in triplex PCR were altered by quadruplex PCR assay.

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Virulence factors in Escherichia coli strains isolated from urinary tract infection and pyometra cases and from feces of healthy dogs

Cited by 74 publications

References 29 publications

Association between virulence profile and fluoroquinolone resistance in Escherichia coli isolated from dogs and cats in China

Association between virulence profile and fluoroquinolone resistance in Escherichia coli isolated from dogs and cats in China

Detection of virulence and antimicrobial resistance genes in Escherichia coli isolates from diarrhoiec dogs in Iran

Phylo-typing of clinicalEscherichia coliisolates originating from bovine mastitis and canine pyometra and urinary tract infection by means of quadruplex PCR

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