Viral DNA sequences in peripheral blood and vertical transmission of the virus: a discussion about BPV-1

Freitas, Antônio Carlos de; Carvalho, Claudemir de; Brunner, O.; Birgel, Eduardo Harry; Dellalibera, Alice Maria Melville Paiva; Benesi, Fernando José; Gregory, Lilian; Beçak, Willy; Stocco, Rita de Cássia

doi:10.1590/s1517-83822003000500026

Cited by 53 publications

(57 citation statements)

References 7 publications

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“…Despite the high frequency of BPV infection, identification of BPV types in Brazilian cattle herds is still sporadic. By using specific primers, BPV type 1 was described in skin warts, peripheral blood and plasma from cattle with cutaneous papillomatosis, while BPV type 2 was detected in whole blood and urinary bladder tumors from cattle with chronic enzootic haematuria and cutaneous papillomatosis (Santos et al 1998, Freitas et al 2003, Wosiacki et al 2005.…”

Section: Introductionmentioning

confidence: 99%

Identification of the recently described new type of bovine papillomavirus (BPV-8) in a Brazilian beef cattle herd

et al. 2009

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RESUMO.-[Identificação do novo tipo de papilomaví-rus bovino (BPV-8) recentemente descrito num rebanho bovino no Brasil.]A primeira descrição do papilomavírus bovino tipo 8 (BPV-8) foi realizada em amostras de papilomas de teto e de pele saudável de tetos de bovinos no Japão. Em 2007, a seqüência genômica completa do BPV-8 foi determinada. Ainda em 2007, uma variante do BPV-8 (BPV-8-EB) foi identificada em lesões papilomatosas de um bisão europeu na Eslováquia. No Brasil, apesar da infecção pelo BPV ser comumente observada em bovinos, a determinação dos tipos virais associados com a infecção ainda é esporádica. Este estudo tem o objetivo de relatar a ocorrência do BPV-8 no país. A amostra clínica foi obtida em um rebanho de corte do estado do Paraná, região sul do Brasil. O papiloma cutâneo, de aspecto macular e não-verrucoso, estava localizado na região dorsal torácica do animal. A identificação do vírus foi realizada pela reação em cadeia da polimerase (PCR) utilizando primers genéri-cos para a amplificação parcial do gene L1. O produto amplificado, com aproximadamente 480 pb, foi clonado e os fragmentos presentes em dois clones foram seqüenciados. A comparação da seqüência de nucleotíde-os com a de outros papilomavírus demonstrou 100% de identidade com o BPV-8. Assim, esta é a primeira descrição da ocorrência do BPV-8 no Brasil, o que sugere a sua presença nos rebanhos bovinos brasileiros.

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Section: Introductionmentioning

confidence: 99%

Identification of the recently described new type of bovine papillomavirus (BPV-8) in a Brazilian beef cattle herd

et al. 2009

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“…However, the presence of viral DNA sequences in different cells such as those of peripheral blood and gametes has been reported (Stocco dos Santos et al, 1998;Carvalho et al, 2003;Freitas et al, 2003). The significance of the presence of these nucleic acid sequences in these tissues has not yet been determined, considering that there is yet no evidence of expression of these nucleic acid sequences and of their potential to initiate infection.…”

Section: Introductionmentioning

confidence: 99%

Can established cultured papilloma cells harbor bovine papillomavirus?

Campos¹,

Trindade²,

Ferraz³

et al. 2008

Genet. Mol. Res.

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ABSTRACT. Papillomaviruses have been reported to be very difficult to grow in cell culture. Also, there are no descriptions of cell cultures from lesions of bovine cutaneous papillomatosis, with identification of different bovine papilloma virus (BPV) DNA sedifferent bovine papilloma virus (BPV) DNA sequences. In the present report, we describe primary cell cultures from samples of cutaneous lesions (warts). We investigated the simultaneous presence of different BPV DNA sequences, comparing the original lesion to different passages of the cell cultures and to peripheral blood. BPV 1, 2 and 4 DNA sequences were found in lesion samples, and respective cell cultures and peripheral blood, supporting our previous hypothesis of the possible activity of these sequences in different samples and now also showing how they can be maintained in different passages of cell cultures.

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“…In humans, sperm transfected with HPV genes are able to penetrate in oocytes, to deliver HPV genome, and HPV genes can be actively transcribed by the penetrate oocyte [10]. Also, the transplacental transmission of PVs appears to take place in humans and cows [13,27,30].This study adds a new dimension to what we know about BPV transmission mechanisms and pathogenesis. The better understanding of the implications of active BPV-containing semen can contribute to improve strategies to avoid BPV dissemination in the cattle herd and its implications in the reproduction.…”

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confidence: 79%

“…The quality of purified DNA was checked by b-globin gene PCR, as previously described [13]. The amplification was carried out in a final volume of 25 lL containing 100 ng of DNA, 19 Master Mix (Promega, USA) and 0.2 lM of specific primers.…”

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confidence: 99%

“…PV-containing blood cells were suggested to be responsible for spreading the infectious agent to numerous organs, including semen [11,29]. This hypothesis may be corroborated by the detection of BPV in different tissues and cells, including reproductive sites as oocytes, the ovary, the uterus, cumulus cells, uterine lavage [6,13,20,36]. It is reasonable to suppose that the presence of active BPV in semen of asymptomatic animals could facilitate the dissemination of BPV through this route.…”

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confidence: 99%

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Bovine papillomavirus E2 and E5 gene expression in sperm cells of healthy bulls

Silva

Gurgel

et al. 2013

VirusDis.

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Papillomaviruses are found in epithelial lesions and are linked to different carcinogenic processes in humans and other animals. Although BPV has been characterized as epitheliotropic, the presence of viral DNA has been detected in other tissues and fluids, such as fresh semen. The aim of this study was to evaluate the presence and expression of BPV in sperm cells of bulls (Bos taurus) asymptomatic for papillomatosis. A PCR assay was carried out with specific primers to test BPV2 in 26 semen samples. The presence of BPV transcripts was assessed by RT-PCR to E2 and E5 genes. BPV2 DNA was detected in nine out of 26 samples and the expression of E2 and E5 were detected in five out of nine BPV positive samples. This is the first record of BPV2 expression in bull sperm cells.Keywords Bovine papillomavirus Á Semen Á Viral gene expression Papillomaviruses (PVs) are double-stranded DNA tumour viruses identified in a broad range of animal species that belongs to the amniotes, including human being [1]. So far, 13 genotypes of Bovine papillomavirus (BPV1-13) have been characterized [21] and classified in three different genera: Deltapapillomaviruses (BPV1 and 2), Epsilonpapillomaviruses (BPV5 and 8) and Xipapillomaviruses (BPV3, 4, 6, 9, 10 and 11). BPV7 is classified as an unassigned PV genus [12,14]. BPVs from Delta-PVs and BPV5 can infect epithelial and subepithelial fibroblasts inducing fibro-epithelial tumours in cattle, known as fibropapillomas while BPVs from another genus can induce true papillomas [22]. Normally, papillomas regress as a result of cellmediate immune response [17]. However, occasionally papillomas may develop in squamous cell carcinoma as a result of involvement of environmental and genetic cofactors [3,5]. Recently, the co-infection among BPVs in skin lesions (warts) has been described, but the real significance of this finding need to be clarified [7,25,31].PVs are described as epitheliotropic viruses, although their presence has been detected in different tissues and cells [11,20,36]. Few studies have contributed to improved understanding of PV transmission, however, its spread through non-epithelial, however, its spreading of through non-epithelial tissues and fluids has been suggested [6,11].In humans, infected sperm are able to penetrate the oocyte, to deliver HPV genome in the oocyte and HPV genes can be actively transcribed by the fertilized oocyte [10]. There are few reports focusing on the study of BPV in reproductive tract and its cells [13,35] although the BPV-1 were described in the prepuce and penis [16] and BPV-2 were found infecting placenta and actively expressed in equine semen [30,34]. In an early study, we demonstrate the high incidence of BPV2 in commercial doses of bull semen from national and multinational companies [33]. However, studies are needed to understand the biological significance of BPV presence in semen. The present study aimed to evaluate the presence of BPV in sperm cells of healthy bull, as well as the viral gene expression. The semen was collected fro...

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Viral DNA sequences in peripheral blood and vertical transmission of the virus: a discussion about BPV-1

Cited by 53 publications

References 7 publications

Identification of the recently described new type of bovine papillomavirus (BPV-8) in a Brazilian beef cattle herd

Identification of the recently described new type of bovine papillomavirus (BPV-8) in a Brazilian beef cattle herd

Can established cultured papilloma cells harbor bovine papillomavirus?

Bovine papillomavirus E2 and E5 gene expression in sperm cells of healthy bulls

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