Violacein and its β-cyclodextrin complexes induce apoptosis and differentiation in HL60 cells

Melo, Patrícia S.; Justo, Giselle Z.; Azevedo, M. B. M. De; Durán, Nélson; Haun, Marcela

doi:10.1016/s0300-483x(02)00751-5

Cited by 82 publications

(60 citation statements)

References 25 publications

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“…Melo et al [17] find that the violacein (IC 50 5-12 µM) is highly cytotoxic to V79 fibroblasts. Saraiva et al [18] find that uveal melanoma cell lines, 92.1 and OCM-1, are found to be sensitive to violacein (GI50 ~1.69-2.21 µM).…”

Section: Resultsmentioning

confidence: 99%

Evaluation of in Vitro Cytotoxic Effect of Violacein Produced by Novel Isolate Chromobacterium Vaccinii Cv5 Against the Cervical and Lung Cancer Cell

Santhosh

Palaniswamy

2017

Asian J Pharm Clin Res

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Objectives: This study investigates the in vitro anticancer activity of the violacein extracted from the Chromobacterium vaccinii CV5.Methods: Natural colorants or dyes derived from flora to fauna are believed to be safe because of nontoxic, noncarcinogenic, and biodegradable in nature. There are a number of natural pigments, but only a few are available in sufficient quantities for industrial production. The cytotoxicity activity of pigment was assessed against the cervical (HeLa) and lung cancer (A549) cell lines using the MTT assay and there by potential cytotoxic activity exhibited by the pigment was identified. Results:The result of the pigment shows potent anticancer activity on the two cancer cell lines tested in a concentration dependent manner. The potent anticancer activity was observed with the pigment with IC 50 values of 26 µg/mL on HeLa and 31 µg/mL on A549 cells, respectively. Conclusion:The study is pioneering report for determining the better in vitro anticancer activity of violacein from the novel isolate C. vaccinii CV5.

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Section: Resultsmentioning

confidence: 99%

Evaluation of in Vitro Cytotoxic Effect of Violacein Produced by Novel Isolate Chromobacterium Vaccinii Cv5 Against the Cervical and Lung Cancer Cell

Santhosh

Palaniswamy

2017

Asian J Pharm Clin Res

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“…A modulator generated the selective pulses and automatically attenuated the power and duration of the pulse. 13 C NMR and heteronuclear single-quantum correlation (HSQC) (DMSO-d6 samples) were carried in 5 mm broadband or triple resonance probes, respectively. 13 C NMR experiments were acquired with 32 k data points, spectral width of 3.0 kHz, and relaxation delays of 1.5 seconds at 125.709 MHz.…”

Section: Spray-drying (Sd)mentioning

confidence: 99%

“…8 The inclusion of the active principles in the cage of β-CD 9,10 protected drugs from absorption in the stomach and the upper portion of the lower intestine and enabled the degradation of the saccharide portion in the large bowel by intestinal microflora, thereby ensuring specific drug delivery with the maximum drug bioavailability. Although investigation into drug inclusion is one of the most extensively researched fields, and complexation and self-assembly of CDs and lipophilic drugs has been investigated for years, [11][12][13] interactions between CDs and hydrophilic drugs have scarcely been reported. [14][15][16] Such an interaction could result in a retarding effect of the associated hydrophilic drug.…”

Section: Introductionmentioning

confidence: 99%

New formulation of an old drug in hypertension treatment: the sustained release of captopril from cyclodextrin nanoparticles

Azevedo¹,

Tasic²,

Fattori³

et al. 2011

IJN

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Captopril (CAP) was the first angiotensin I-converting enzyme (ACE) inhibitor to be developed and is widely used in hypertension treatment. On the other hand, cyclodextrins (CDs) are cyclic oligosaccharides whose cone-shaped cavity allows formation of noncovalent inclusion complexes with appropriately sized guest molecules, thus modifying guest physical, chemical, and biological properties. Herein, the physicochemical characterization and in vivo ACE inhibition evaluation of seven CAP/CD complexes are reported. The inclusion complexes were prepared by spray-drying, freeze-drying, kneading, or lyophilization methods and characterized by nuclear magnetic resonance, Fourier-transformed infrared spectroscopy, X-ray diffraction, differential scanning calorimetry, and scanning electron microscopy techniques. In vivo assays compared CAP and CAP/CD complex administration (0.5 mg kg −1 or 0.09 mg kg −1 , n = 4–7) to evaluate the ACE inhibition by continuous infusion of angiotensin I (30 ng 50 μL −1 min −1 ) in conscious Wistar rats. The physicochemical analysis demonstrated complete amorphization and complexation between CAP and CDs, indicating the substitution of water molecules inside the CD cavity with CAP. During the infusion of angiotensin I, the administration of all CAP/CD complexes induced a reduction in mean arterial pressure similar to that observed upon CAP administration. The nanoparticles obtained by the kneading method (CAP/α-CD:KM) showed a potent and long-lasting inhibitory activity (∼22 hours) on the angiotensin I pressor effect. The results suggest that the inclusion complex of CAP and α-CD can function as a novel antihypertensive formulation that may improve therapeutic use of CAP by reducing its oral dose administration to once per day, thus providing better quality of life for almost 25% of the world’s population who suffer from hypertension.

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“…1) This bacterium produces a large amount of violacein, a purple pigment that has strong anti-trypanosome and anti-tumor properties. 2,3) The results of our recent study suggest that violacein production in strain 520P1 is regulated by quorum sensing. Quorum sensing is a phenomenon in which bacteria use signaling molecules, termed autoinducers, to communicate with each other.…”

mentioning

confidence: 99%

Quorum Sensing Signaling Molecules Involved in the Production of Violacein byPseudoalteromonas

Wang

Ikawa

Okaue

et al. 2008

Bioscience, Biotechnology, and Biochemistry

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Violacein and its β-cyclodextrin complexes induce apoptosis and differentiation in HL60 cells

Cited by 82 publications

References 25 publications

Evaluation of in Vitro Cytotoxic Effect of Violacein Produced by Novel Isolate Chromobacterium Vaccinii Cv5 Against the Cervical and Lung Cancer Cell

Evaluation of in Vitro Cytotoxic Effect of Violacein Produced by Novel Isolate Chromobacterium Vaccinii Cv5 Against the Cervical and Lung Cancer Cell

New formulation of an old drug in hypertension treatment: the sustained release of captopril from cyclodextrin nanoparticles

Quorum Sensing Signaling Molecules Involved in the Production of Violacein byPseudoalteromonas

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