Mungbean (V. radiata) is an important Asiatic legume supplying inexpensive protein to a vast majority of vegetarian masses. To increase markers repertoire in mungbean, a study was conducted to analyse 384 microsatellite markers derived from common bean, scarlet runner bean and adzuki bean for their transferability and polymorphism. The results showed that 87 (24.71%) primer pairs could amplify DNA loci of 20 mungbean genotypes including one accession of V. trilobata, while 52 showed reliable banding and polymorphism. These showed different degrees of variability at each locus producing 250 alleles with the number of alleles varying from 2 to 9. The major allele frequency varied from 0.17 to 0.95, while the polymorphic information content of SSRs ranged between 0.09 and 0.86 with an average of 0.60 AE 0.16. UPGMA revealed three major clusters accommodating~95% of the accessions while one accession of V. trilobata ('NSB-007') did not group with any other genotype describing the discriminating power of informative microsatellites. This study identified a set of useful microsatellite markers to accelerate the genetic studies and breeding programme of mungbean.Key words: Vigna radiata -simple sequence repeat markersPhaseolus coccineus Mungbean (Vigna radiata var. radiata) is an inexpensive source of protein for vegetarian diets. It is extensively grown in south and south-east Asia, while its largest production comes from India. In 2013-14, it was grown on about 3.38 m ha in the country with a production of 1.60 mt. It has a short crop cycle, low input requirements and ability to survive in a wide range of adverse soil and climatic conditions and therefore can be grown across different climates (spring, summer, winter and rainy seasons) in different parts of the subcontinent as a sole, relay, or intercrop (Pratap et al. 2014a,b). Summer cultivation of mungbean is a rewarding experience which is becoming increasingly popular among Asian farmers. However, susceptibility of mungbean to yellow mosaic disease (YMD) caused by mungbean yellow mosaic virus (MYMV) and mungbean yellow mosaic India virus (MYMIV), and its sensitivity to photo-thermo periods and infestation of thrips, especially during reproductive phase, limit its production considerably. Further, being confined to the Asian regions, this crop has received little attention from global research community till now and is less studied as compared to other grain legumes such as chickpea, pigeon pea, common bean and soybean. Use of molecular markers in this crop is further still limited due to slow development of genomic resources such as availability of polymorphic trait-specific markers.An understanding of the genetic and genomic relationships of extant mungbean species and cultivars is critical for the further utilization of mungbean genetic diversity and genomic information in the development of superior cultivars. There are different genetic approaches for assessment of genetic diversity in germplasm accessions, breeding lines and segregating populations b...