Vibrio cholerae hemagglutinin/protease, colonial variation, virulence, and detachment

Abstract

The structural gene, hap, for the secreted hemagglutinin/protease (HA/protease), a putative virulence factor of Vibrio cholerae, has recently been cloned and sequenced (C. C. Hase and R. A. Finkelstein, J. Bacteriol. 173:3311-3317, 1991). The availability of the null mutant, HAP-1, and HAP-1 complemented with pCH2 (which expresses HA/protease), enabled an examination of the role of HA/protease in the virulence of V. cholerae in an animal model. However, the mutants exhibited reversible colonial variation similar but not identical to that which was previously associated with dramatic changes in virulence of parental strain 3083. Regardless of colonial morphology, the mutants were found to be fully virulent in infant rabbits. Thus, the HA/protease is not a primary virulence factor (for infant rabbits). Observations using cultured human intestinal cells indicated, instead, that the HA/protease is responsible for detachment of the vibrios from the cultured cells by digestion of several putative receptors for V. cholerae adhesins.

Interaction between Aspergillus fumigatus and basement membrane laminin: Binding and substrate degradation

“…In our study, both the A and B chains of laminin were rapidly degraded, whereas entactin-nidogen seemed to be more resistant to proteolysis. Additional studies will be needed to demonstrate, as postulated for pathogenic amoeba [38] or Vibrio cholerae [15], if this degradation occurs at cell contact sites and if laminin fragments released from basement membranes may secondary bound to conidia, thus allowing attachment-detachment, migration and subsequent colonization of tissues.…”

Vibrio cholerae in a historically cholera‐free country

“…All strains encoded hlyA, rtxA, HA/P, which are involved in V. cholerae virulence in humans (Finkelstein et al, 1992;Olivier et al, 2007). Fifty-eight strains (45%) encoded cholix toxin, a novel ADP-ribosylating toxin and 114 (89%) also encoded the integrase of an integrative and conjugative element (ICE).…”

Purification and characterization of a novel metalloprotease isolated from Aeromonas caviae

“…Pseudomonas aeruginosa elastase cleaved IgA, increasing lung inflammation and stimulating alginate synthesis, and the correlation between the production of protese and the clinical state of cystic fibrosis have been reported [10]. A range of protease produced by members of the family Vibrionaceae [11,12] are considered to be involved in the pathogenesis.…”

“…Analysis of protein digestion by AP19. Each substrate was incubated in the absence (lane3,5,7,9,11,13) or in the presence (lane 4, 6, 8, 10, 12, 14) of AP19 at 37°C for 3 h. Lane 1, molecular size standard; lane 2, purified AP19; lane 3-4, Bovine thyloglobulin (BTG); lane 5-6, human lacto ferine (HLF); lane 7-8, collagen; lane 9-10, elastin; lane 11-12, bovine serum albumin (BSA); lane 13-14, rabbit immunoglobulin G (IgG). Digested substrates were separated on a 12% SDS-PAGE gel.…”