Vibrational spectroscopic analysis of blood for diagnosis of infections and sepsis: a review of requirements for a rapid diagnostic test

Confield, Lily Rose; Black, George; Wilson, Brian C.; Lowe, David J.; Theakstone, Ashton G.; Baker, Matthew J.

doi:10.1039/d0ay01991g

Cited by 8 publications

(8 citation statements)

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“…Indeed, sepsis-related mortality is largely preventable with timely diagnosis and rapid treatment using effective antimicrobial agents [ 6 ]. Unfortunately, there is a lack of rapid, robust diagnostic methods to detect the invading pathogens that cause sepsis [ 7 ]. The current “gold standard” diagnostic method, blood culture, has variable sensitivity (50%-80%) and can take hours to days to yield results [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Common Bacterial Isolates Associated With Neonatal Sepsis and Their Antimicrobial Profile: A Retrospective Study at King Abdulaziz University Hospital, Jeddah, Saudi Arabia

Alharbi¹

2022

Cureus

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BackgroundNeonatal sepsis is a major contributor to morbidity and mortality among neonates. There has been considerable geographic variation in causative pathogens and antimicrobial sensitivity profiles over time. This makes the continuous monitoring of patterns of emergence crucial for the effective implementation of antimicrobial therapy guidelines in an attempt to control antimicrobial resistance. MethodsA retrospective study was conducted among neonates with sepsis admitted to King

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Section: Introductionmentioning

confidence: 99%

Common Bacterial Isolates Associated With Neonatal Sepsis and Their Antimicrobial Profile: A Retrospective Study at King Abdulaziz University Hospital, Jeddah, Saudi Arabia

Alharbi¹

2022

Cureus

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“…For diagnosing a bacterial illness, microbial cultures are the "gold standard" (6), Due to the fact that sepsis is frequently caused by infection, the diagnosis of sepsis can be confirmed by performing a blood culture to identify the pathogenic bacteria. However, reports show that the positive blood culture rate is only about 10% in infectious diseases and 30-40% in patients with sepsis (7)(8)(9). The Journal of the American Medical Association published the third international consensus on the definition of sepsis and septic shock in 2016 (10), defining sepsis as life-threatening organ dysfunction caused by a dysregulated host response to infection, with organ dysfunction defined as an acute change in sequential organ failure assessment (SOFA) ≥2 caused by infection, i.e., Sepsis = Infection + SOFA ≥ 2.…”

Section: Introductionmentioning

confidence: 99%

The diagnostic utility of IL-10, IL-17, and PCT in patients with sepsis infection

Zhang

Wang

Hou

et al. 2022

Front. Public Health

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ObjectiveThe purpose of this study is to determine the diagnostic value and net clinical benefit of interleukin-10 (IL-10), interleukin-17 (IL-17), procalcitonin (PCT), and combination tests in patients with sepsis, which will serve as a standard for sepsis early detection.Patients and methodsAn investigation of 84 sepsis patients and 81 patients with local inflammatory diseases admitted to the ICU of Tongji University Hospital in 2021. In addition to comparing inter-group variability, indicators relevant to sepsis diagnosis and therapy were screened.ResultsLASSO regression was used to examine PCT, WBC, CRP, IL-10, IFN-, IL-12, and IL-17. Multivariate logistic regression linked IL-10, IL-17, and PCT to sepsis risk. The AUC values of IL-10, IL-17, PCT, and the combination of the three tests were much higher than those of standard laboratory infection indicators. The combined AUC was greater than the sum of IL-10, IL-17, and PCT (P < 0.05). A clinical decision curve analysis of IL-10, IL-17, PCT, and the three combined tests found that the three combined tests outperformed the individual tests in terms of total clinical benefit rate. To predict the risk of sepsis using IL-10, IL-17, and PCT had an AUC of 0.951, and the model's predicted probability was well matched. An examination of the nomogram model's clinical value demonstrated a considerable net therapeutic benefit between 3 and 87%.ConclusionThe IL-10, IL-17, and PCT tests all have a high diagnostic value for patients with sepsis, and the combination of the three tests outperforms the individual tests in terms of diagnostic performance, while the combined tests have a higher overall clinical benefit rate.

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“…74 Label-free Raman spectroscopy can overcome these shortcomings by implementing a fast and cheap tool for blood analysis with little sample preparation. 75 For instance, Wang et al established an assay based on SERS for fast and quantitative detection of interleukin 6 (IL-6). 76 In the case of infection stimulation, IL-6 is an appropriate predictor of severe sepsis.…”

Section: ■ Raman Spectroscopy For Diagnosticsmentioning

confidence: 99%

Diagnostics and Therapy Assessment Using Label-Free Raman Imaging

El-Mashtoly

Gerwert

2021

Anal. Chem.

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Vibrational spectroscopic analysis of blood for diagnosis of infections and sepsis: a review of requirements for a rapid diagnostic test

Abstract: Infections and sepsis represent a growing global burden. There is a widespread clinical need for a rapid, high-throughput and sensitive technique for the diagnosis of infections and detection of invading...

Cited by 8 publications

References 123 publications

Common Bacterial Isolates Associated With Neonatal Sepsis and Their Antimicrobial Profile: A Retrospective Study at King Abdulaziz University Hospital, Jeddah, Saudi Arabia

Common Bacterial Isolates Associated With Neonatal Sepsis and Their Antimicrobial Profile: A Retrospective Study at King Abdulaziz University Hospital, Jeddah, Saudi Arabia

The diagnostic utility of IL-10, IL-17, and PCT in patients with sepsis infection

Diagnostics and Therapy Assessment Using Label-Free Raman Imaging

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