Vibrational microspectroscopy analysis of human lenses

Paluszkiewicz, C.; Piergies, Natalia; Sozańska, Agnieszka; Chaniecki, Piotr; Rękas, Marek; Miszczyk, Justyna; Gajda, Mariusz; Kwiatek, Wojciech M.

doi:10.1016/j.saa.2017.07.027

Cited by 22 publications

(13 citation statements)

References 40 publications

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“…According to the literature, this is associated with the contents of water in the nuclear lenses [17]. The various content of water in the nucleus for the normal lens in comparison with the tamoxifen treated one is observed and indicated a cataract lens that in agreement with Paluszkiewicz et al, [18] in a study illustrated IR spectra to normal lens and cataractous one.…”

Section: Disscutionsupporting

confidence: 69%

Ftir Spectroscopic Trends and Dna Damage in Rabbit Lens Due to Long Run of Tamoxifen Treatment

Mervat

Eman

2019

Int J App Pharm

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Objective: The aim of the present work is to evaluate the molecular structure changes of the lens of rabbits and DNA damage of epithelial cells due to tamoxifen administration. Methods: Twenty four healthy New Zealand white rabbits were divided into 2 main groups. The first group is served as control (n=12) kept untreated, second one is Tamoxifen administrative group (n=12) received orally daily dose of 15 mg/kg. Rabbits were decapitated after 2, 4, 6 and 8 mo, respectively. Using fourior transform infrared (FTIR) to study the molecular structure changes due to tamoxifen and comet assay analysis for discovering DNA damage. Results: FTIR data indicated that tamoxifen affects structural components in NHOH and fingerprint region. Increases of β-turns of the protein secondary structure while, reducing the content of both α-helix after 8 mo and Turns appeared for all periods of administrative tamoxifen were observed. On the other hand tamoxifen induced a statistically significant increase in comet assay parameters as tail moment compared to control animals that indicated DNA damage due to single or double strand break. Conclusion: Tamoxifen uses for more than 6 mo may lead to changes in the molecular structure of the lens and damage of DNA cells. An ophthalmic baseline examination prior to anti-cancer treatment may help detect any pre-existing ocular condition and lead to reduction of ocular side effects when predisposed patients are screened and examined regularly during and after chemotherapeutic therapy.

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Section: Disscutionsupporting

confidence: 69%

Ftir Spectroscopic Trends and Dna Damage in Rabbit Lens Due to Long Run of Tamoxifen Treatment

Mervat

Eman

2019

Int J App Pharm

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“…Bond length (Å) Bonds Angle (°) Bonds Dihedral angle (°) C 1 -C 2 1.204 C 2 -C 3 -C 8 119.5 C 6 -C 7 -N 9 -C 10 − 179.3 C 2 -C 3 1.430 C 8 -C 7 -N 9 124.5 N 9 -C 10 -C 11 -C 12 1.071 C 3 -C 4 1.403 N 9 -C 10 -N 19 120.0 C 11 -C 12 -C 13 -O 20 174.9 C 4 -C 5 1.390 N 9 -C 10 -C 11 118.8 C 16 -C 15 -C 14 -O 25 − 179.1 C 5 -C 6 1.388 C 10 -C 11 12 1.414 C 15 -C 14 -O 25 125.0 C 12 -C 13 1.374 C 14 -O 25 -C 26 118.9 C 13 -C 14 1.433 O 25 -C 26 -C 27 108.4 C 14 -C 15 1.378 C 26 -C 27 -O 28 109.4 C 15 -C 16 1.414 C 27 -O 28 -C 29 112.8 C 16 -N 17 1.367 N 17 -C 18 1.310 C 18 -N 19 1.351 N 19 -C 10 1.323 C 11 -C 16 1.418 C 13 -O 20 1.364 O 20 -C 21 1.441 C 21 -C 22 1.519 C 22 -O 23 1.418 O 23 -C 24 1.412 C 14 -O 25 1.356 O 25 -C 26 1.428 C 26 -C 27 1.511 C 27 -O 28 1.412 O 28 -C 29 1.414…”

Section: Bondmentioning

confidence: 99%

“…ese studies present vibrational spectroscopy characterization by means of Raman spectroscopy (RS) and Fourier-transform infrared absorption spectroscopy (FTIR) of the molecular structure of erlotinib in the solid state and solution in different pH conditions. ese two complementary methods are commonly used for qualitative and quantitative analysis of different types of samples in various conditions and physical states [10][11][12][13][14][15]. RS and FTIR are governed by different quantum mechanical selection rules [16].…”

Section: Introductionmentioning

confidence: 99%

Vibrational Fingerprint of Erlotinib: FTIR, RS, and DFT Studies

Piergies

Paluszkiewicz

Kwiatek

2019

Journal of Spectroscopy

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In this study, we provide the first Fourier-transform infrared absorption spectroscopy (FTIR) and Raman spectroscopy (RS) analysis of a vibrational fingerprint of erlotinib, a drug which is applied in non-small cell lung cancer therapy, in solid state and solution in different pH conditions. Additionally, the performed DFT theoretical calculations in vacuum and PCM models support the interpretation of vibrational spectra and give insight into an optimized spatial configuration of the investigated drug. e present considerations show vibrational structure of erlotinib and details of its molecular geometry. Furthermore, we discuss the pH condition where the protonated -NH + and C�N + forms occur and indicate the spectral changes characteristic for the erlotinib protonation. It is of great of importance to better understand biological activity of the drug and to develop new tyrosine kinase inhibitors.

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“…Previously, FTIR microspectroscopy was used to study the whole lens [25][26][27][28][29] . However, FTIR spectroscopy and microscopy were not used to study human LC LECs until now, up to our knowledge.…”

mentioning

confidence: 99%

Synchrotron-based FTIR microspectroscopy of protein aggregation and lipids peroxidation changes in human cataractous lens epithelial cells

Kreuzer

Dučić

Hawlina

et al. 2020

Sci Rep

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Cataract is the leading cause of blindness worldwide but the mechanisms involved in the process of cataractogenesis are not yet fully understood. Two most prevalent types of age-related cataracts are nuclear (N) and cortical (C) cataracts. A common environmental factor in most age-related cataracts is believed to be oxidative stress. The lens epithelium, the first physical and biological barrier in the lens, is build from lens epithelial cells (LECs). LECs are important for the maintenance of lens transparency as they control energy production, antioxidative mechanisms and biochemical transport for the whole lens. The purpose of this study is to characterize compounds in LECs originated from N and C cataracts, by using the synchrotron radiation-based Fourier Transform Infrared (SR-FTIR) microspectroscopy, in order to understand the functional importance of their different bio-macromolecules in cataractogenesis. We used the SR-FTIR microspectroscopy setup installed on the beamline MIRAS at the Spanish synchrotron light source ALBA, where measurements were set to achieve single cell resolution, with high spectral stability and high photon flux. The results showed that protein aggregation in form of fibrils was notably pronounced in LECs of N cataracts, while oxidative stress and the lipids peroxidation were more pronounced in LECs of C cataracts.

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Vibrational microspectroscopy analysis of human lenses

Cited by 22 publications

References 40 publications

Ftir Spectroscopic Trends and Dna Damage in Rabbit Lens Due to Long Run of Tamoxifen Treatment

Ftir Spectroscopic Trends and Dna Damage in Rabbit Lens Due to Long Run of Tamoxifen Treatment

Vibrational Fingerprint of Erlotinib: FTIR, RS, and DFT Studies

Synchrotron-based FTIR microspectroscopy of protein aggregation and lipids peroxidation changes in human cataractous lens epithelial cells

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