Viability of Human Corneal Endothelium Following Optisol-GS Storage

Porcine Cornea Storage Ex Vivo Model as an Alternative to Human Donor Tissues for Investigations of Endothelial Layer Preservation

“…Previous studies have reported EC mortality ranging from 0.5% to 11% 4 , 24 , 38 after 14 days of hypothermic storage, and the present study's results, although using a different method that investigates a macroscopic rather than a microscopic image, are comparable to these findings. In addition, the present method allows analysis of EC mortality in the totality of the endothelial area, including the periphery, while microscopic evaluation usually examines fields in the central area.…”

Porcine Cornea Storage Ex Vivo Model as an Alternative to Human Donor Tissues for Investigations of Endothelial Layer Preservation

“…Previous studies have reported EC mortality ranging from 0.5% to 11% 4 , 24 , 38 after 14 days of hypothermic storage, and the present study's results, although using a different method that investigates a macroscopic rather than a microscopic image, are comparable to these findings. In addition, the present method allows analysis of EC mortality in the totality of the endothelial area, including the periphery, while microscopic evaluation usually examines fields in the central area.…”

Assessment of performance and safety of Corneal Chamber hypothermic storage medium and PSS-L corneal rinsing solution in human and porcine corneas

“…28 In our analysis endothelial mortality slightly increased at the end of storage in both human and porcine corneas, by an average of 1.9% and 5.2%, respectively. This percentage was comparable 29 or lower 30 than those observed in studies with other hypothermic storage media at 14 days. In the presented analysis, corneal transparency decreased in human and porcine corneas after 14 days, by an average of 9.4% and 10.7%, respectively, in line with similar changes previously reported.…”

Fibroblast growth factor-2 protects endothelial cells from damage after corneal storage at 4°C

“…Means et al calculated the rate of endothelial damage in human corneas to be 0.57%±0.30% per day for stor- age times from 4 to 21 days [22]. This would represent a damage rate of 8.55% after a storage time of 15 days, which is significantly less than the 13.37% rate of damage found in control corneas in our present study.…”

Porcine Cornea Storage Ex Vivo Model as an Alternative to Human Donor Tissues for Investigations of Endothelial Layer Preservation

“…Previous studies have reported EC mortality ranging from 0.5% to 11% 4 , 24 , 38 after 14 days of hypothermic storage, and the present study's results, although using a different method that investigates a macroscopic rather than a microscopic image, are comparable to these findings. In addition, the present method allows analysis of EC mortality in the totality of the endothelial area, including the periphery, while microscopic evaluation usually examines fields in the central area.…”

Assessment of performance and safety of Corneal Chamber hypothermic storage medium and PSS-L corneal rinsing solution in human and porcine corneas

“…28 In our analysis endothelial mortality slightly increased at the end of storage in both human and porcine corneas, by an average of 1.9% and 5.2%, respectively. This percentage was comparable 29 or lower 30 than those observed in studies with other hypothermic storage media at 14 days. In the presented analysis, corneal transparency decreased in human and porcine corneas after 14 days, by an average of 9.4% and 10.7%, respectively, in line with similar changes previously reported.…”

Fibroblast growth factor-2 protects endothelial cells from damage after corneal storage at 4°C

“…Means et al calculated the rate of endothelial damage in human corneas to be 0.57%±0.30% per day for stor- age times from 4 to 21 days [22]. This would represent a damage rate of 8.55% after a storage time of 15 days, which is significantly less than the 13.37% rate of damage found in control corneas in our present study.…”

Porcine Cornea Storage Ex Vivo Model as an Alternative to Human Donor Tissues for Investigations of Endothelial Layer Preservation

“…Previous studies have reported EC mortality ranging from 0.5% to 11% 4 , 24 , 38 after 14 days of hypothermic storage, and the present study's results, although using a different method that investigates a macroscopic rather than a microscopic image, are comparable to these findings. In addition, the present method allows analysis of EC mortality in the totality of the endothelial area, including the periphery, while microscopic evaluation usually examines fields in the central area.…”

Assessment of performance and safety of Corneal Chamber hypothermic storage medium and PSS-L corneal rinsing solution in human and porcine corneas

“…28 In our analysis endothelial mortality slightly increased at the end of storage in both human and porcine corneas, by an average of 1.9% and 5.2%, respectively. This percentage was comparable 29 or lower 30 than those observed in studies with other hypothermic storage media at 14 days. In the presented analysis, corneal transparency decreased in human and porcine corneas after 14 days, by an average of 9.4% and 10.7%, respectively, in line with similar changes previously reported.…”

Fibroblast growth factor-2 protects endothelial cells from damage after corneal storage at 4°C

“…Means et al calculated the rate of endothelial damage in human corneas to be 0.57%±0.30% per day for stor- age times from 4 to 21 days [22]. This would represent a damage rate of 8.55% after a storage time of 15 days, which is significantly less than the 13.37% rate of damage found in control corneas in our present study.…”