Viability of fibroblasts cultured under nutritional stress irradiated with red laser, infrared laser, and red light-emitting diode

“Aroeira” (Myracrodruon urundeuva) methanol extract: the relationship between chemical compounds and cellular effects

“…The cells were treated with this solution (50 lg/ mL) and the incubation plate for 3 h at 37 C to stain lysosomes of the viable cells. The cells were washed with PBS-Ca þ2 , and the stain was extracted in a 50% ethanol solution and 1% acetic acid, adding 200 lL of this solution per well (Volpato et al 2011). Next, the optical density was determined at 540 nm (FluoStar OPTIMA, microplate reader, BMG LABTECH, Ortenberg, Germany).…”

The relative antimicrobial effect of blue 405 nm LED and blue 405 nm laser on methicillin-resistant Staphylococcus aureus in vitro

“…The low cost of LEDs compared to lasers, and their ubiquity, suggests that LED-based devices will remain preferred by many over lasers, unless there is strong evidence that intervention with lasers yield superior results. Consequently, the photobiomodulative effect of LEDs relative to lasers has been questioned by many [1,2], with some asserting that laser diodes are more effective than LEDs [3,4] and others claiming that LEDs are as effective as lasers [1,[4][5][6][7][8].…”

Viability of Human Gingival Fibroblast (FGH) Treated with Ethanolic "Aroeira" Extract (Myracrodruon urundeuva Allemão)

“…The cells were treated with this solution (50 µg/mL) and the plates were incubated at 37 o C for 3h to allow the stain to be absorbed by the lysosomes in viable cells. The cells were then washed with phosphate buffered saline with calcium (PBSCa +2 ) and the stain was extracted with a 50% ethanol solution and 1% acetic acid by placing 200 µL of this solution in each well (Volpato et al 2011). Then, the absorbance was determined at 540 nm (FluoStar OPTIMA microplate fluorescence reader).…”