Viability and Reproducibility of Periodontal Ligament Cells on Avulsed Teeth Stored in Ham's F-10 Solution

Talebi, Maryam; Parisay, Iman; Afshari, Jalil Tavakol; Shajiei, Arezoo; Ghadim, Mostafa Sofiani

doi:10.17796/1053-4628-42.3.6

Cited by 8 publications

(5 citation statements)

References 17 publications

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“…In the experimental studies, the teeth were typically allowed to dry for about 30 minutes (in mud or sand) to simulate the first aid situation prior to storing them in the solutions or films for 15 minutes to 24 hours. It is of note that only five experimental studies stored the extracted teeth for longer than 120 minutes 28–32 …”

Section: Resultsmentioning

confidence: 99%

“…Two studies reported on the effectiveness of tap water for storing an extracted tooth. One RCT including 30 extracted teeth for each comparison showed less benefit for cell viability after 60‐minute to 24‐hour immersion in tap water compared with milk 32 . One non‐RCT did not report the number of extracted teeth in the study groups but also showed that the extracted teeth stored in tap water for 45 minutes had a lower cell viability rate than those stored in milk 41 .…”

Section: Resultsmentioning

confidence: 99%

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Storage of an avulsed tooth prior to replantation: A systematic review and meta‐analysis

Brier

Dorien

Borra³

et al. 2020

Dental Traumatology

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Background/Aim It is crucial to store an avulsed tooth appropriately to preserve the viability of the periodontal ligament cells prior to replantation. The aim of this systematic review was to identify the best available evidence for the effectiveness of any technique available to laypeople for storing an avulsed tooth compared with storage in milk or saliva. Methods The following databases were searched (September 2019): Cochrane Library, MEDLINE, and Embase. Two reviewers independently considered trial eligibility, then extracted and analyzed data, and assessed the risk of bias. The certainty of the body of evidence was appraised according to the GRADE methodology. Results Out of 4118 references, 33 studies were included and reported 23 comparisons of which 10 were synthesized in a meta‐analysis. The limited evidence available favors storing an avulsed tooth in Hank's balanced salt solution (pooled SMD 2.47, 95% CI [1.59;3.34], P < .00001), propolis solution (pooled SMD 1.73, 95% CI [1.12;2.33], P < .00001), oral rehydration salts (pooled SMD 4.16, 95% CI [2.10;6.23], P < .0001), rice water, and cling film compared with storage in milk. The cell viability rate was significantly lower in teeth stored in saline solutions (pooled SMD −4.35, 95% CI [−7.55;−1.14], P = .008), tap water, buttermilk, castor oil, GC Tooth Mousse, and turmeric extract than those stored in milk. There is insufficient evidence to recommend for or against temporary storage of an avulsed tooth in saliva compared with alternative solutions. The certainty of evidence was considered low to very low due to limitations in study design, indirect study populations and outcome measures, and imprecision. Conclusion Although milk was shown to extend the periodontal ligament cell viability before replantation compared with saline or tap water, the following media have also demonstrated efficacy at preserving the cell viability: Hank's balanced salt solution, propolis, oral rehydration salts, rice water, and cling film.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Storage of an avulsed tooth prior to replantation: A systematic review and meta‐analysis

Brier

Dorien

Borra³

et al. 2020

Dental Traumatology

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“…The First Aid Task Force requested a 2020 SysRev of media used to store an avulsed tooth until it can be reimplanted. 111 Many RCTs found benefit from immersion of the tooth in Hanks’ Balanced Salt Solution 146 , 147 , 148 , 149 , 150 , 151 , 152 , 153 , 154 , 155 , 156 , 157 as well as in oral rehydration salt solutions 154 , 155 or from wrapping the tooth in cling film (ie, plastic wrap) 158 as compared with immersion in milk. However, milk was better than many other media for storing a tooth until reimplantation.…”

Section: First Aidmentioning

confidence: 99%

Executive Summary 2020 International Consensus on Cardiopulmonary Resuscitation and Emergency Cardiovascular Care Science With Treatment Recommendations

Nolan¹,

Maconochie²,

Soar³

et al. 2020

Resuscitation

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requiring first aid, related education and implementation strategies, and systems of care. After the publication of the 2015 International Consensus on CPR and ECC Science With Treatment Recommendations, ILCOR also committed to sponsoring a continuous evidenceevaluation process, with topics prioritized for review by the task forces and with CoSTR updates published annually. For this 2020 CoSTR, the 6 ILCOR task forces performed structured reviews of 184 topics, completing the most ambitious evidence review that ILCOR has attempted to date.The ILCOR systematic review process continues to be based on the methodological principles published by the National Academy of Health and Medicine (formerly the Institute of Medicine) 2 ; Cochrane 3,4 ; Grading of Recommendations Assessment, Development, and Evaluation (GRADE) 5 ; and the reporting guidelines based on the Preferred Reporting Items for Systematic Reviews and Meta-Analyses recommendations. 6,7 Three types of evidence evaluation provided the basis for this 2020 CoSTR: the systematic review, the scoping review, and the evidence update. Based on recommendations from the ILCOR Scientific Affairs Committee and agreement of the task forces, only systematic reviews could result in new or modified treatment recommendations.

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“…We obtained 31 full texts to further verify their eligibility. After evaluating the full texts, 11 studies were excluded due to six reasons: ineligible topic (n = 5), no full text available (n = 1), duplicate records (n = 2), ineligible media (n = 1), ineligible outcome (n = 1), and inadequate data (n = 1), and then 20 eligible studies (6,(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35)(36)(37)(38)(39)(40)(41) including 31 reports were considered to be eligible for our inclusion criteria. The identification and selection of studies were Figure 1.…”

Section: Identification and Selection Of Studiesmentioning

confidence: 99%

“…Among the 20 included studies, only three studies (31,38,39) definitively reported the methods of generating random sequence, none of the studies (6,(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35)(36)(37)(38)(39)(40)(41) reported the details of performing allocation concealment, and all studies were rated as low risk of bias in incomplete data, selective reporting, and other bias domains. Overall, the level of risk of bias among all studies was considered to be moderate.…”

Section: Risk Of Biasmentioning

confidence: 99%

Network Meta-Analysis of 10 Storage Mediums for Preserving Avulsed Teeth

Zhang

Cheng

et al. 2021

Front. Med.

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Many storage mediums are available for the storage of avulsed teeth to preserve the viability of periodontal ligament (PDL) cells before replantation; however, it is unclear which medium is the optimal option. We performed this network meta-analysis to answer this question. A comprehensive search was conducted in PubMed, EMBASE, and the Cochrane library to capture eligible studies investigating the comparative efficacy of Hank's balanced salt solution (HBSS), aloe vera gel (AVG), oral rehydration solution (ORS), coconut water, egg white, green tea, propolis, saline, milk, and water. Statistical analysis was conducted using Review Manager v5.3 and ADDIS v1.16.8. In total, 20 RCTs involving 31 reports were included finally. Direct meta-analysis suggested that HBSS was superior to ORS, milk, saline, and water, ORS was superior to milk but inferior to coconut water and propolis, egg white was superior to milk but inferior to AVG and propolis, propolis was superior to AVG, milk, and saline, and coconut water and water was inferior to saline and milk, respectively. Network meta-analysis suggested that AVG was inferior to the other nine mediums, and propolis was superior to HBSS (SMD, −5260.24; 95% CrI, −10447.39 to −70.37) and milk (SMD, −5461.11; 95% CrI, −10574.99 to −328.51). Moreover, ranking probabilities indicated the highest probability for propolis, followed by saline, ORS, HBSS, milk, egg white, water, green tea, and AVG successively. Propolis may be the optimal media for storing avulsed teeth before replantation. However, given the availability of propolis and HBSS and the hypotonic properties of saline, ORS or milk should also be preferentially selected.

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Viability and Reproducibility of Periodontal Ligament Cells on Avulsed Teeth Stored in Ham's F-10 Solution

Abstract: Ham's F-10 is capable of preserving PDL cells viable and reproducible better than milk and tap water and similar to HBSS.

Cited by 8 publications

References 17 publications

Storage of an avulsed tooth prior to replantation: A systematic review and meta‐analysis

Storage of an avulsed tooth prior to replantation: A systematic review and meta‐analysis

Executive Summary 2020 International Consensus on Cardiopulmonary Resuscitation and Emergency Cardiovascular Care Science With Treatment Recommendations

Network Meta-Analysis of 10 Storage Mediums for Preserving Avulsed Teeth

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