2012
DOI: 10.1021/pr200872s
|View full text |Cite
|
Sign up to set email alerts
|

Abstract: Microorganisms release effector molecules that modulate the host machinery enabling survival, replication, and dissemination of a pathogen. Here we characterized the extracellular proteome of Paracoccidioides brasiliensis at its pathogenic yeast phase. Cell-free culture supernatants from the Pb18 isolate, cultivated in defined medium, were separated into vesicle and vesicle-free fractions, digested with trypsin and analyzed by liquid chromatography-tandem mass spectrometry. In vesicle and vesicle-free preparat… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1

Citation Types

13
192
1
5

Year Published

2014
2014
2019
2019

Publication Types

Select...
6
3

Relationship

1
8

Authors

Journals

citations
Cited by 154 publications
(211 citation statements)
references
References 66 publications
13
192
1
5
Order By: Relevance
“…Like for those previously described in C. neoformans and other fungal species, we found a large functional distribution of identified hits (23)(24)(25)(26)(27). The distribution carried through all the size-enriched populations, suggesting that any loading mechanism is size independent.…”
Section: Discussionsupporting
confidence: 84%
“…Like for those previously described in C. neoformans and other fungal species, we found a large functional distribution of identified hits (23)(24)(25)(26)(27). The distribution carried through all the size-enriched populations, suggesting that any loading mechanism is size independent.…”
Section: Discussionsupporting
confidence: 84%
“…We identified between 179 and 278 unique proteins per strain. As with previous extracellular vesicle proteomics analyses, the proteins identified were associated with a wide variety of cellular functions and localizations (10,13,15,21,22). The 179 proteins identified in the wild-type EVs were in line with previously described C. albicans proteins (11).…”
Section: Resultssupporting
confidence: 78%
“…Fungal extracellular vesicles (EVs) were isolated from culture supernatants as described in a number of studies by sequential centrifugation steps (8,9,11,13,14,35). Briefly, the cells and debris were removed from culture fluids by centrifugation at 5,000 and 15,000 ϫ g (15 min, 4°C).…”
Section: Methodsmentioning
confidence: 99%
“…Remarkably, most of the molecules trafficked by fungi to the extracellular milieu lack secretion signals (5-7). Extracellular fungal molecules include numerous proteins (8)(9)(10)(11) but also pigments (12) and polysaccharides (13,14). It is now well accepted that these molecules are at least partially exported to the outer space in EVs (15).…”
mentioning
confidence: 99%