Very Late Antigen-1 Mediates Corneal Lymphangiogenesis

Grimaldo, Sammy; Yuen, Don; Ecoiffier, Tatiana; Chen, Lu

doi:10.1167/iovs.10-6580

Cited by 24 publications

(51 citation statements)

References 35 publications

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“…To induce inflammatory lymphangiogenesis and hemangiogenesis in the cornea, we employed the standard suture placement model, as described previously [6][7][8] Supplementary information, Data S1. Corneal lymphatic vessels were visualized by subconjunctival injection of the large molecular weight fluorescein isothiocyanatelabeled dextran [9].…”

Section: Dear Editormentioning

confidence: 99%

Live imaging of newly formed lymphatic vessels in the cornea

Yuen

Kwan

et al. 2011

Cell Res

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Section: Dear Editormentioning

confidence: 99%

Live imaging of newly formed lymphatic vessels in the cornea

Yuen

Kwan

et al. 2011

Cell Res

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“…[20][21][22][23] Briefly, three 11-0 nylon sutures (AROSurgical, Newport Beach, CA) were placed into central corneas at equal distance ratio (suture to limbus/corneal radius) in all mice. Seven or 14 days later, whole-mount flat corneas were collected for immunohistochemical studies on macrophages, and LG and HG, respectively.…”

Section: Induction Of Corneal Inflammatory Lymphangiogenesis and Hemamentioning

confidence: 99%

“…[21][22][23][24] Briefly, freshly excised corneas were fixed in acetone for immunofluorescent staining. Nonspecific staining was blocked with anti-Fc CD16/CD32 antibody (BD Biosciences, San Jose, CA) and donkey serum ( Jackson ImmunoResearch Laboratories Inc. West Grove, PA).…”

Section: Immunofluorescent Microscopic Assaymentioning

confidence: 99%

“…[21][22][23][24][25] Basically, vessels in each sample were evaluated using 4 parameters: invasion area, density, diameter, and branching points. The invasion area was presented in a percentage coverage score obtained by normalizing the vascular area to the total corneal area.…”

Section: Vascular and Macrophage Quantificationmentioning

confidence: 99%

“…Macrophage infiltration was also evaluated according to the standard protocol. 22,26,27 Briefly, total 10 areas (8 from the periphery and 2 from the center) of each sample were randomly picked and examined under the epifluorescence microscope. Total numbers of F4/80 positive cells were counted throughout the whole thickness of the picked area.…”

Section: Vascular and Macrophage Quantificationmentioning

confidence: 99%

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Increased Lymphangiogenesis and Hemangiogenesis in Infant Cornea

Yuen

Leu

Sadovnikova

et al. 2011

Lymphatic Research and Biology

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Background: Corneal lymphangiogenesis (LG) and hemangiogenesis (HG) accompany many diseases after inflammatory, infectious, traumatic or chemical insults. They also contribute to transplant rejection. It is known that corneal transplants in infants or children have a higher rejection rate than in adults. However, it has never been studied whether infant corneas differ from adult corneas in inflammatory LG, HG, or both, which is the focus of this study. Methods and Results: Corneal inflammatory LG and HG were induced by a standard suture placement model in C57BL/6 mice of 3 weeks and 8 weeks of age, respectively. Corneal LG, HG, and macrophage infiltration were assessed by immunofluorescent microscopic studies using specific antibodies against CD31 (a panendothelial cell marker), LYVE-1 (a lymphatic marker), and F4/80 (a macrophage marker). Blood vessels were also examined by ophthalmic slit-lamp microscopic assays in vivo. Digital images were analyzed by NIH Image J software. It was found, for the first time, that infant corneas exhibited a higher level of LG, HG, and macrophage infiltration during inflammation. Infant lymphatic and blood vessels demonstrated greater density and invasion area but similar branching points. Additionally, infant lymphatic vessels were also of larger diameter. Conclusions: Infant and adult corneas differ greatly in their inflammatory responses of LG, HG, and macrophage infiltration. These novel findings will shed some light on our understanding of the LG and HG processes, as well as the development of new therapeutic protocols for corneal diseases, particularly, in infants or children, where an early restoration of sight is critically important in preventing amblyopia or permanent vision loss.

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Contribution of very late antigen-4 (VLA-4) integrin to cancer progression and metastasis

Schlesinger

Bendas

2015

Cancer Metastasis Rev

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The integrin "very late antigen-4" (VLA-4) is expressed by numerous cells of hematopoietic origin and possesses a key function in the cellular immune response, e.g., by mediating leukocyte tethering, rolling, binding, and finally transmigration of the vascular wall at inflammatory sites. Thus, VLA-4 is a valuable target in medical sciences to interfere with pathological inflammations. In addition, leukemic cells and different solid tumors, which express VLA-4, make use of these adhesive functions and confer VLA-4 a progressive role in the metastatic spread. With a growing insight into the molecular mechanisms for creating a tumor-friendly microenvironment at metastatic sites and various tumor host interactions, the multiple functions of VLA-4 became evident recently, e.g., in leukocyte recruitment to micrometastases, the protection of tumors from immune surveillance, or contribution to a chemoresistance. Nevertheless, despite accumulating evidence for several functions of VLA-4 in tumorigenicity, a therapeutic interference with VLA-4 in cancer sciences has not been developed yet to the clinical level, undoubtedly by a marked impact on the physiological immune response. This review gives an up to date insight into the multiple functional role of VLA-4 in cancer and introduces this integrin as a promising target worthwhile to attract attention in biomedical cancer research.

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Very Late Antigen-1 Mediates Corneal Lymphangiogenesis

Abstract: These novel findings together indicate that VLA-1 is critically involved in the processes of lymphangiogenesis. Further investigation on this factor may provide novel therapies for corneal inflammation, transplant rejection, and other lymphatic-related disorders in the body.

Cited by 24 publications

References 35 publications

Live imaging of newly formed lymphatic vessels in the cornea

Live imaging of newly formed lymphatic vessels in the cornea

Increased Lymphangiogenesis and Hemangiogenesis in Infant Cornea

Contribution of very late antigen-4 (VLA-4) integrin to cancer progression and metastasis

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