Vasodilator effects of cromakalim and HA 1077 in diabetic rat aorta

Gürpınar, Tuğba; Gök, Şule

doi:10.4414/smw.2012.13558

Cited by 4 publications

(2 citation statements)

References 29 publications

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“…The effectiveness of the removal of the endothelium was confirmed by the absence of relaxation. Sustained relaxation (70% of precontracted tone) in response to ACh confirmed the presence of a functional endothelium [40] .…”

Section: Vascular Tone Of Rat Aortic Ringsmentioning

confidence: 80%

The prolactin family hormones regulate vascular tone through NO and prostacyclin production in isolated rat aortic rings

et al. 2015

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Aim: Prolactin family hormones include growth hormone, placental lactogen and prolactin, which are able to regulate angiogenesis via NO and prostaglandins. However, their effects on vascular tone are not fully understood. The aim of this study was to evaluate the effects of prolactin family hormones on rat vascular tone in vitro. Methods: Aortic rings were prepared from adult male rats and precontracted with phenylephrine, then treated with the hormones and drugs. The tension was measured with isometric force displacement transducer connected to a polygraph. NO production and prostacyclin release in physiological solution was determined. Cultured rat aortic endothelial cells (RAECs) were treated with the hormones and drugs, and the phosphorylation of eNOS at serine 1177 was assessed using Western bolt analysis. Results: Administration of growth hormone or placental lactogen (0.01-100 nmol/L) induced endothelium-dependent vasodilation. Both the hormones significantly increased the phosphorylation of eNOS in RAECs and NO level in physiological solution. Preincubation with L-NAME blocked growth hormone-or placental lactogen-induced vasodilation and NO production. Preincubation with an antibody against growth hormone receptors blocked growth hormone-and placental lactogen-induced vasodilation. Addition of a single dose of prolactin (0.01 nmol/L) induced sustained vessel relaxation, whereas multiple doses of prolactin induced a biphasic contractionrelaxation effect. The vascular effects of prolactin depended on endothelium. Prolactin significantly increased the level of prostacyclin I 2 in physiological solution. Preincubation with indomethacin or an antibody against prolactin receptors blocked prolactin-induced vasodilation. Conclusion: The prolactin family hormones regulate rat vascular tone, selectively promoting either relaxation or contraction of vascular smooth muscle via activation of either growth hormone receptors or prolactin receptors within the endothelium.

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Section: Vascular Tone Of Rat Aortic Ringsmentioning

confidence: 80%

The prolactin family hormones regulate vascular tone through NO and prostacyclin production in isolated rat aortic rings

et al. 2015

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“…The effectiveness of removal of the endothelium was confirmed through the absence of relaxation or the maintenance of the contractile effect [49]. Sustained relaxation reported as more than 70% of precontracted tone in the presence of ACh ensures the presence of endothelium [50].…”

Section: Methodsmentioning

confidence: 96%

Effect of the double bond conjugation on the vascular physiology and nitric oxide production of isomers of eicosapentaenoic and docosahexaenoic acids prepared from shark oil

et al. 2020

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A collection of evidence suggests that conjugation of double bonds of eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids, omega-3 polyunsaturated fatty acids (n-3 PUFAs), increases their anticarcinogenic activity; however, the effect of such conjugation on vascular tone activity remains unknown. We propose that the mixture of conjugated PUFAs exerts higher vasorelaxation activity than the corresponding mixture of nonconjugated PUFAs. The vascular response to different concentrations of conjugated and nonconjugated isomers of EPA and DHA, among other fatty acids (FAs) naturally present in shark oil, and the role of nitric oxide (NO) as a vasorelaxant agent were investigated. Both conjugated EPA (CEPA) and conjugated DHA (CDHA) were prepared by alkaline isomerization of all PUFAs contained in shark oil. Different concentrations of conjugated and nonconjugated PUFAs were placed in contact with precontracted aortic rings of Wistar rats to assess their effect on vascular tone. All tested samples exerted a vasorelaxant effect. Compared to nonconjugated PUFAs, conjugated isomers exhibited an increase in the dilatation of the aortic rings (P<0.001) in a dose-dependent manner (P<0.001). In addition, nonconjugated PUFAs produced nitric oxide (NO) in a dose-dependent manner, while conjugated PUFAs did not, suggesting that their dilatation mechanism is not totally dependent on NO.

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Exploring the therapeutic promise of targeting Rho kinase in rheumatoid arthritis

et al. 2021

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Vasodilator effects of cromakalim and HA 1077 in diabetic rat aorta

Abstract: These results suggest that the impaired relaxant effects were further decreased depending on K(ATP) channel activity but the effects of Rho-kinase enzyme inhibitors on relaxation responses were not significantly changed in diabetes mellitus.

Cited by 4 publications

References 29 publications

The prolactin family hormones regulate vascular tone through NO and prostacyclin production in isolated rat aortic rings

The prolactin family hormones regulate vascular tone through NO and prostacyclin production in isolated rat aortic rings

Effect of the double bond conjugation on the vascular physiology and nitric oxide production of isomers of eicosapentaenoic and docosahexaenoic acids prepared from shark oil

Exploring the therapeutic promise of targeting Rho kinase in rheumatoid arthritis

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