Vasoactive Intestinal Peptide-Induced Expression of Cytochrome P450 Cholesterol Side-Chain Cleavage and 17α-Hydroxylase Enzyme Activity in Hen Granulosa Cells1

Johnson, A. L.; Li, Z; Gibney, Jean A.; Malamed, Sasha

doi:10.1095/biolreprod51.2.327

Cited by 32 publications

(23 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In summary, the related paracrine/autocrine factors, activin A and TGF␤1, represent additional factors (together with circulating FSH and possibly paracrine-acting vasoactive intestinal peptide) [38] that are proposed to initiate hen granulosa cell differentiation and, by implication, follicle selection into the preovulatory hierarchy. Accordingly, a current focus is to investigate additional cellular sites of MAP kinase inhibition together with mechanisms that are ultimately responsible for releasing granulosa cells from such inhibition within those follicles selected for ovulation.…”

Section: Discussionmentioning

confidence: 99%

Cellular Mechanisms and Modulation of Activin A- and Transforming Growth Factor β-Mediated Differentiation in Cultured Hen Granulosa Cells1

Johnson

Bridgham

Woods

2004

Biology of Reproduction

View full text Add to dashboard Cite

Undifferentiated granulosa cells from prehierarchal (6- to 8-mm-diameter) hen follicles express very low to undetectable levels of LH receptor (LH-R) mRNA, P450 cholesterol side chain cleavage (P450scc) enzyme activity, and steroidogenic acute regulatory (StAR) protein, and produce negligible progesterone, in vitro, following an acute (3-h) challenge with either FSH or LH. It has previously been established that culturing such cells with FSH for 18-20 h induces LH-R, P450scc, and StAR expression, which enables the initiation of progesterone production. The present studies were conducted to characterize the ability of activin and transforming growth factor (TGF) beta, both alone and in combination with FSH, to promote hen granulosa cell differentiation, in vitro. A 20-h culture of prehierarchal follicle granulosa cells with activin A or transforming growth factor beta (TGFbeta)1 increased LH-R mRNA levels compared with control cultured cells. Activin A and TGFbeta1 also promoted FSH-receptor (FSH-R) mRNA expression when combined with FSH treatment. Neither activin A nor TGFbeta1 alone stimulated progesterone production after 20 h culture. However, preculture with either factor for 20 h (to induce gonadotropin receptor mRNA expression) followed by a 3-h challenge with FSH or LH potentiated StAR expression and progesterone production compared with cells challenged with gonadotropin in the absence of activin A or TGFbeta1 preculture. Significantly, activation of the mitogen-activated protein (MAP) kinase pathway with transforming growth factor alpha (TGFalpha) (monitored by Erk phosphorylation) blocked TGFbeta1-induced LH-R expression, and this effect was associated with the inhibition of Smad2 phosphorylation. We conclude that a primary differentiation-inducing action of activin A and TGFbeta1 on hen granulosa cells from prehierarchal follicles is directed toward LH-R expression. Enhanced LH-R levels subsequently sensitize granulosa cells to LH, which in turn promotes StAR plus P450scc expression and subsequently an increase in P4 production. Significantly, the finding that TGFbeta signaling is negatively regulated by MAP kinase signaling is proposed to represent a mechanism that prevents premature differentiation of granulosa cells.

show abstract

Section: Discussionmentioning

confidence: 99%

Cellular Mechanisms and Modulation of Activin A- and Transforming Growth Factor β-Mediated Differentiation in Cultured Hen Granulosa Cells1

Johnson

Bridgham

Woods

2004

Biology of Reproduction

View full text Add to dashboard Cite

show abstract

“…The effect of VIP in stimulating androgen secretion from DII, EP, and E ovaries may be reflective of the ability of VIP to increase P450 SSC enzyme complex, the rate-limiting enzyme in P 4 biosynthesis (Trzeciak et al 1986, Johnson et al 1994. The maximal VIP-induced release of androgen secretion occurred from the EPovaries, the cycle stage where the activity of SSC complex is limiting to induce a large spontaneous increase in ovarian androgen release (Freeman 1994).…”

Section: Vip and Cyclic Steroid Productionmentioning

confidence: 99%

Participation of vasoactive intestinal polypeptide in ovarian steroids production during the rat estrous cycle and in the development of estradiol valerate-induced polycystic ovary

Parra

Fiedler²,

Luna³

et al. 2007

Reproduction

View full text Add to dashboard Cite

Vasoactive intestinal polypeptide (VIP) stimulates estradiol and progesterone release from ovarian granulosa cells in vitro. Very little information is available as to the role VIP plays in the control of steroid secretion during reproductive cyclicity and in ovarian pathologies involving altered steroid secretion. In this study, we determined the involvement of VIP in regulating ovarian androgen and estradiol release during estrous cyclicity and estradiol valerate (EV)-induced polycystic ovarian development in rats. Our findings show that androgen and estradiol release from ovaries obtained during different stages of rat estrous cycle mimic cyclic changes in steroid release observed in vivo with maximal release occurring during late proestrus. VIP increased androgen release from ovaries of all cycle stages except late proestrus and estradiol release from all cycle stages. Increases in VIP-induced androgen and estradiol release were maximal at early proestrus. Inclusion of saturating concentrations of androstenedione increased magnitude of VIP-induced estradiol release at diestrus and estrus but not proestrus. Magnitude of VIP-induced androgen and estradiol release tended to be greater in the ovaries from EV-treated rats with polycystic ovary compared with estrous controls. At the tissue level, ovarian VIP concentration was cycle stage dependent with highest level seen in diestrus. Maximum concentration of VIP was found in EV-treated rats. Changes in VIP were inversely related to changes in ovarian nerve growth factor, a neuropeptide involved in ovarian androgen secretion. These results strongly suggest that intraovarian VIP participates in the control of estradiol secretion during the rat estrous cycle and possibly in the maintenance of increased ovarian estradiol secretory activity of EV-treated rats.

show abstract

“…This results in the inability of granulosa to produce progesterone (P4; the primary steroid produced by the granulosa layer) following a 3-h challenge of freshly collected cells with ovine FSH [3]. Luteinizing hormone (LH) receptor (LHR) mRNA expression within the granulosa layer is detected only after follicle selection and is initiated by cAMP signaling in response to FSH and possibly vasoactive intestinal peptide [6][7][8]. By comparison, the adjacent theca layer from prehierarchal follicles expresses readily detectable levels of both FSHR [1] and LHR mRNA [7], and responds to either FSH or LH treatment in vitro, with increased steroid production [9,10].…”

Section: Introductionmentioning

confidence: 99%

Bone Morphogenetic Protein 4 Supports the Initial Differentiation of Hen (Gallus gallus) Granulosa Cells

Kim

Ocón-Grove

Johnson

2013

Biology of Reproduction

Self Cite

View full text Add to dashboard Cite

In the hen ovary, selection of a follicle into the preovulatory hierarchy occurs from a small cohort of prehierarchal (6-8 mm) follicles. Prior to follicle selection the granulosa layer remains in an undifferentiated state despite elevated follicle-stimulating hormone receptor (FSHR) expression. The present studies describe a role for bone morphogenetic protein 4 (BMP4) in supporting FSHR mRNA expression in granulosa cells from prehierarchal follicles and promoting differentiation at follicle selection. Culture of undifferentiated granulosa cells in culture medium alone resulted in a significant decline in levels of FSHR mRNA (by ~80% compared to freshly collected cells). By comparison, granulosa cultured with BMP4 (10-100 ng/ml) maintained FSHR and expression at approximately in vivo levels. Because both granulosa and theca tissues from prehierarchal follicles express BMP4, it is suggested that BMP4 acts in a paracrine and/or autocrine fashion to support elevated FSHR expression prior to follicle selection. Granulosa cells cultured with BMP4 for 24 h also initiated FSH-induced cAMP production and indirectly initiated anti-Mullerian hormone (AMH), CYP11A, and STAR expression plus progesterone production. However, pretreatment with the BMP antagonist NOGGIN or the mitogen-activated protein kinase (MAPK) agonist transforming growth factor alpha attenuated or blocked each action promoted by BMP4. We conclude that prior to and immediately after selection, BMP4 serves to support FSHR expression within the granulosa layer, yet prior to selection, multiple factors (including inhibitory MAPK signaling, AMH, and BMP antagonists) can modulate FSHR expression and suppress FSH-mediated cell signaling to prevent granulosa cell differentiation prior to follicle selection.

show abstract

Vasoactive Intestinal Peptide-Induced Expression of Cytochrome P450 Cholesterol Side-Chain Cleavage and 17α-Hydroxylase Enzyme Activity in Hen Granulosa Cells1

Cited by 32 publications

References 26 publications

Cellular Mechanisms and Modulation of Activin A- and Transforming Growth Factor β-Mediated Differentiation in Cultured Hen Granulosa Cells1

Cellular Mechanisms and Modulation of Activin A- and Transforming Growth Factor β-Mediated Differentiation in Cultured Hen Granulosa Cells1

Participation of vasoactive intestinal polypeptide in ovarian steroids production during the rat estrous cycle and in the development of estradiol valerate-induced polycystic ovary

Bone Morphogenetic Protein 4 Supports the Initial Differentiation of Hen (Gallus gallus) Granulosa Cells

Contact Info

Product

Resources

About