Vasculitis-like syndrome associated with Borrelia lusitaniae infection

Carvalho, Isabel Lopes de; Fonseca, João Eurico; Marques, José Gonçalo; Ullmann, Amy J.; Hojgaard, Andrias; Zeidner, Nordin S.; Núncio, Maria Sofia

doi:10.1007/s10067-008-1012-z

Cited by 49 publications

(29 citation statements)

References 11 publications

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“…All B. lusitaniae isolates analyzed in the present study were readily killed by complement-active human serum, arguing for the inability of this genospecies to infect and survive in the human host. However, B. lusitaniae has been isolated from two Lyme disease patients, suggesting that it may have some pathogenic potential in humans (9)(10)(11) (4,6,19,27,34,57). As observed for B. garinii non-OspA serotype 4, B. lusitaniae strains also group with the serum-sensitive borreliae.…”

Section: Fig 3 Binding Of Serum Proteins By B Lusitaniae Isolatesmentioning

confidence: 99%

Inadequate Binding of Immune Regulator Factor H Is Associated with Sensitivity ofBorrelia lusitaniaeto Human Complement

et al. 2010

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Spirochetes belonging to the Borrelia burgdorferi sensu lato complex differ in resistance to complementmediated killing by human serum. Here, we characterize complement sensitivity of a panel of B. lusitaniae isolates derived from ticks collected in Germany and Portugal as well as one patient-derived isolate, PoHL. All isolates are highly susceptible to complement-mediated lysis in human serum and activate complement predominantly by the alternative pathway, leading to an increased deposition of complement components C3, C6, and the terminal complement complex. Interestingly, serum-sensitive B. lusitaniae isolates were able to bind immune regulator factor H (CFH), and some strains also bound CFH-related protein 1 (CFHR1) and CFHR2. Moreover, CFH bound to the surface of B. lusitaniae was inefficient in mediating C3b conversion. Furthermore, the identification and characterization of a potential CFH-binding protein, OspE, revealed that this molecule possesses a significantly reduced binding capacity for CFH compared to that of CFH-binding OspE paralogs expressed by various serum-resistant Borrelia species. This finding suggests that a reduced binding capability of CFH is associated with an increased serum sensitivity of B. lusitaniae to human complement.

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Section: Fig 3 Binding Of Serum Proteins By B Lusitaniae Isolatesmentioning

confidence: 99%

Inadequate Binding of Immune Regulator Factor H Is Associated with Sensitivity ofBorrelia lusitaniaeto Human Complement

et al. 2010

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“…Of the 18 genospecies of this complex (Margos et al, 2011), B. afzelii, B. garinii, B. spielmanii, B. bavariensis , and B. burgdorferi sensu stricto have already been detected in The Netherlands, in both patients and questing ticks. B. lusitaniae , and B. valaisiana were detected in questing I. ricinus , but their public health significance is less clear (Collares-Pereira et al, 2004; Diza et al, 2004; De Carvalho et al, 2008; Coipan et al, 2013). …”

Section: Introductionmentioning

confidence: 99%

Spatiotemporal dynamics of emerging pathogens in questing Ixodes ricinus

Coipan¹,

Jahfari²,

Fonville³

et al. 2013

Front. Cell. Infect. Microbiol.

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Ixodes ricinus transmits Borrelia burgdorferi sensu lato, the etiological agent of Lyme disease. Previous studies have also detected Rickettsia helvetica, Anaplasma phagocytophilum, Neoehrlichia mikurensis, and several Babesia species in questing ticks in The Netherlands. In this study, we assessed the acarological risk of exposure to several tick-borne pathogens (TBPs), in The Netherlands. Questing ticks were collected monthly between 2006 and 2010 at 21 sites and between 2000 and 2009 at one other site. Nymphs and adults were analysed individually for the presence of TBPs using an array-approach. Collated data of this and previous studies were used to generate, for each pathogen, a presence/absence map and to further analyse their spatiotemporal variation. R. helvetica (31.1%) and B. burgdorferi sensu lato (11.8%) had the highest overall prevalence and were detected in all areas. N. mikurensis (5.6%), A. phagocytophilum (0.8%), and Babesia spp. (1.7%) were detected in most, but not all areas. The prevalences of pathogens varied among the study areas from 0 to 64%, while the density of questing ticks varied from 1 to 179/100 m2. Overall, 37% of the ticks were infected with at least one pathogen and 6.3% with more than one pathogen. One-third of the Borrelia-positive ticks were infected with at least one other pathogen. Coinfection of B. afzelii with N. mikurensis and with Babesia spp. occurred significantly more often than single infections, indicating the existence of mutual reservoir hosts. Alternatively, coinfection of R. helvetica with either B. afzelii or N. mikurensis occurred significantly less frequent. The diversity of TBPs detected in I. ricinus in this study and the frequency of their coinfections with B. burgdorferi s.l., underline the need to consider them when evaluating the risks of infection and subsequently the risk of disease following a tick bite.

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“…The most common agents of human LB are Borrelia burgdorferi (sensu stricto), Borrelia afzelii, Borrelia garinii, Borrelia lusitaniae, and Borrelia spielmanii (7,8,12,35). To date, 15 species have been named within the group of LB spirochetes (6,31,32,37,38,41).…”

mentioning

confidence: 99%

A NewBorreliaSpecies Defined by Multilocus Sequence Analysis of Housekeeping Genes

Margos

Vollmer

Cornet

et al. 2009

Appl Environ Microbiol

259

221

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Analysis of Lyme borreliosis (LB) spirochetes, using a novel multilocus sequence analysis scheme, revealed that OspA serotype 4 strains (a rodent-associated ecotype) of Borrelia garinii were sufficiently genetically distinct from bird-associated B. garinii strains to deserve species status. We suggest that OspA serotype 4 strains be raised to species status and named Borrelia bavariensis sp. nov. The rooted phylogenetic trees provide novel insights into the evolutionary history of LB spirochetes.Multilocus sequence typing (MLST) and multilocus sequence analysis (MLSA) have been shown to be powerful and pragmatic molecular methods for typing large numbers of microbial strains for population genetics studies, delineation of species, and assignment of strains to defined bacterial species (4,13,27,40,44). To date, MLST/MLSA schemes have been applied only to a few vector-borne microbial populations (1,6,30,37,40,41,47).Lyme borreliosis (LB) spirochetes comprise a diverse group of zoonotic bacteria which are transmitted among vertebrate hosts by ixodid (hard) ticks. The most common agents of human LB are Borrelia burgdorferi (sensu stricto), Borrelia afzelii, Borrelia garinii, Borrelia lusitaniae, and Borrelia spielmanii (7,8,12,35). To date, 15 species have been named within the group of LB spirochetes (6,31,32,37,38,41). While several of these LB species have been delineated using whole DNA-DNA hybridization (3, 20, 33), most ecological or epidemiological studies have been using single loci (5, 9-11, 29, 34, 36, 38, 42, 51, 53). Although some of these loci have been convenient for species assignment of strains or to address particular epidemiological questions, they may be unsuitable to resolve evolutionary relationships among LB species, because it is not possible to define any outgroup. For example, both the 5S-23S intergenic spacer (5S-23S IGS) and the gene encoding the outer surface protein A (ospA) are present only in LB spirochete genomes (36, 43). The advantage of using appropriate housekeeping genes of LB group spirochetes is that phylogenetic trees can be rooted with sequences of relapsing fever spirochetes. This renders the data amenable to detailed evolutionary studies of LB spirochetes.

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Vasculitis-like syndrome associated with Borrelia lusitaniae infection

Cited by 49 publications

References 11 publications

Inadequate Binding of Immune Regulator Factor H Is Associated with Sensitivity ofBorrelia lusitaniaeto Human Complement

Inadequate Binding of Immune Regulator Factor H Is Associated with Sensitivity ofBorrelia lusitaniaeto Human Complement

Spatiotemporal dynamics of emerging pathogens in questing Ixodes ricinus

A NewBorreliaSpecies Defined by Multilocus Sequence Analysis of Housekeeping Genes

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