Vascularized synovial flap promoting regeneration of the cryopreserved meniscal allograft: experimental study in rabbits

Yamazaki, Kaoru; Tachibana, Yomei

doi:10.1007/s007760300010

Cited by 20 publications

(14 citation statements)

References 19 publications

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“…The resulting 1.8% fibrin hydrogel was printed into parallel channels on untreated tissue culture plastic, allowed to polymerize for 15 minutes, and covered by another layer of 1.8% fibrin to permit cell migration. Fibrin has been used extensively as a stimulus for meniscal repair, both in experimental studies and in clinical practice (23)(24)(25)(26)(27)(28).…”

Section: Methodsmentioning

confidence: 99%

Endothelial Cells Enhance the Migration of Bovine Meniscus Cells

Yuan

Eng

Arkonac

et al. 2014

Arthritis & Rheumatology

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Objective To study the interactions between vascular endothelial cells and meniscal fibrochondrocytes from the inner avascular and outer vascular regions of the meniscus, and identify angiogenic factors that enhance cell migration and integrative repair. Methods Bovine meniscal fibrochondrocytes (bMFCs) from the inner and outer regions of meniscus were cultured for seven days with and without human umbilical vein endothelial cells (HUVECs) in a micropatterned three-dimensional hydrogel system for cell migration. Angiogenic factors secreted by HUVECs were probed for their role in paracrine mechanisms governing bMFC migration, and applied to a full-thickness defect model of meniscal repair in explants from the inner and outer regions over four weeks. Results Endothelial cells enhanced migration of inner and outer bMFCs in the micropatterned system via endothelin-1 (ET-1) signaling. Supplementation of ET-1 significantly enhanced integration strength of full-thickness defects in inner and outer explants, and cell migration at the macro-scale, compared to controls without ET-1 treatment. Conclusion We report for the first time that bMFCs from both the avascular and vascular regions respond to the presence of endothelial cells with increased migration. Paracrine signaling by endothelial cells regulates the bMFCs differentially by region, but we identify ET-1 as an angiogenic factor that stimulates migration of inner and outer cells at the micro-scale, and integrative repair of inner and outer explants at the macro-scale. These findings reveal the regional interactions between vasculature and MFCs, and suggest ET-1 as a potential new treatment modality for avascular meniscal injuries, in order to prevent the development of osteoarthritis.

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Section: Methodsmentioning

confidence: 99%

Endothelial Cells Enhance the Migration of Bovine Meniscus Cells

Yuan

Eng

Arkonac

et al. 2014

Arthritis & Rheumatology

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“…Similar data were also obtained in the rabbit model: the use of fresh menisci showed that both immediate and delayed meniscal allograft transplantation offer some initial protection to the cartilaginous surfaces of the knee (Cummins et al ., ); however, delayed meniscal allograft transplantation leads to more graft shrinkage than immediate allograft transplantation (Rijk and Van Noorden ). Deep‐frozen menisci have also been tested in the rabbit model and were found to be improved by association with a vascularized synovial flap: this approach promoted the invasion of repair tissue into the deeper matrix substance from the surface of the graft (Yamazaki and Tachibana ). No immune reaction or allograft rejection was described in these animal models, and this could be explained by the immune privilege of these grafts (Canham and Stanish, ).…”

Section: Meniscal Replacement and Regenerationmentioning

confidence: 99%

Animal models for meniscus repair and regeneration

Deponti

Giancamillo

Scotti

et al. 2013

J Tissue Eng Regen Med

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The meniscus plays an important role in knee function and mechanics. Meniscal lesions, however, are common phenomena and this tissue is not able to achieve spontaneous successful repair, particularly in the inner avascular zone. Several animal models have been studied and proposed for testing different reparative approaches, as well as for studying regenerative methods aiming to restore the original shape and function of this structure. This review summarizes the gross anatomy, function, ultrastructure and biochemical composition of the knee meniscus in several animal models in comparison with the human meniscus. The relevance of the models is discussed from the point of view of basic research as well as of clinical translation for meniscal repair, substitution and regeneration. Finally, the advantages and disadvantages of each model for various research directions are critically discussed.

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“…In animal studies, this method resulted in increased healing in middle one-third meniscus repairs but no increase in healing with central one-third tears. 27 25 A slightly more complex method is to suture a vascularized pedicle of synovium into a meniscus repair.…”

Section: Mechanical Enhancement Of Healingmentioning

confidence: 99%

Biological Enhancement of Meniscus Repair and Replacement

Anz

Rodkey

2012

Sports Medicine and Arthroscopy Review

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When a meniscus injury occurs, it is generally accepted that preserving the meniscus is important for life-long joint preservation. Traditional suture repair of the meniscus has good results; however, the healing potential of meniscus tissue remains as a biological challenge because it is not a completely vascularized structure. For this reason, investigators have continued to search for adjuncts to improve clinical results. Mechanical adjuncts, local factor enhancement, scaffolds, gene therapy, and cell therapy have all been examined as options for improvement of biology and structure. This study reviews the basic science and clinical application of these modalities and provides an assessment of techniques on the horizon.

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Vascularized synovial flap promoting regeneration of the cryopreserved meniscal allograft: experimental study in rabbits

Cited by 20 publications

References 19 publications

Endothelial Cells Enhance the Migration of Bovine Meniscus Cells

Endothelial Cells Enhance the Migration of Bovine Meniscus Cells

Animal models for meniscus repair and regeneration

Biological Enhancement of Meniscus Repair and Replacement

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