Vascular smooth muscle cell response on thin films of collagen

Elliott, John T.; Woodward, John T.; Langenbach, Kurt J.; Tona, Alessandro; Jones, Peter; Plant, Anne L.

doi:10.1016/j.matbio.2005.07.005

Cited by 56 publications

(60 citation statements)

References 49 publications

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“…1͑c͒-1͑f͔͒ images are consistent with previously published images of collagen fibrils formed at alkanethiolate monolayers. 9,11,14 These images indicate that both PS and alkanethiolate monolayers support the formation of the supramolecular fibrillar structure of type 1 collagen and that these fibrils appear to be randomly distributed across both surfaces. The phase images show that the density of the fibrils on both the alkanethiolate monolayer and PS surface are similar.…”

Section: A Analysis Of Collagen Filmsmentioning

confidence: 86%

“…Various cell types have been reported to respond differently to fibrillar versus nonfibrillar collagen with respect to cell spreading. 9,15,24 The ␤1 integrin binding specificity for both nonfibrillar and fibrillar collagen 14,25 provides evidence for the role of the supramolecular structure of the collagen as critical to eliciting cell response. The role of the mechanical properties of the collagen fibrils in determining cell spreading has been implicated by observing greater cell spreading on stiffened collagen fibrils.…”

Section: Discussionmentioning

confidence: 99%

“…Expression of TN-C is modulated by the ECM composition 14,15,20 and by ECM mechanical properties. 4,21,22 TN-C expression is down-regulated in cells on collagen fibrils 14,15,23 or otherwise under treatments that inhibit cell division or prevent spreading. 6 So that the TN-C response of cells could be easily observed on the different substrates, stable transfectants ͑designated as TN-CdsEGFP͒ were prepared using a DNA construct consisting of the TN-C gene promoter fused to the sequence for dsEGFP.…”

Section: Tenascin-c Promoter Activitymentioning

confidence: 99%

“…12 We have demonstrated that vascular smooth muscle cells and NIH 3T3 fibroblasts respond to films of collagen in a manner that is apparently identical to their response to thick gels of collagen by examining integrin engagement, proliferation rates, gene expression, ERK phosphorylation, morphology, and cytoskeletal organization. 14,15 Such data can provide mechanistic understanding of cell response to collagen in tissue and scaffolding, and how cell response to other environmental factors is influenced by ECM conditions.…”

Section: Introductionmentioning

confidence: 99%

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Evaluating the performance of fibrillar collagen films formed at polystyrene surfaces as cell culture substrates

et al. 2008

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While it is well-appreciated that the extracellular matrix plays a critical role in influencing cell responses, well-defined and reproducible presentation of extracellular matrix proteins poses a challenge for in vitro experiments. Films of type 1 collagen fibrils assembled on alkanethiolate monolayers formed at gold-coated surfaces have been shown to elicit a cellular response comparable to collagen gels, but with the advantages of excellent optical properties, and high reproducibility and robustness. To make this collagen matrix more accessible to laboratories that do not have access to gold film deposition the authors have examined the use of untreated polystyrene as a substrate for forming fibrillar collagen films. Direct comparison of films of fibrillar collagen fibrils formed at polystyrene with those formed at alkanethiolate monolayers indicates that films of collagen formed on these two surfaces compare very favorably to one another, both in their supramolecular structural characteristics as well as in the cell response that they elicit. Both substrates exhibit a dense covering of fibrils approximately 200 nm in diameter. The spreading of fibroblasts and activation of the tenascin-C gene promoter are statistically equivalent as determined by a metric derived from the D-statistic normally used in the Kolmogorov-Smirnov statistical test. The results of this study suggest that biologically relevant, robust thin films of collagen fibrils can be formed in any laboratory in untreated polystyrene dishes and multi-well polystyrene plates.

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Section: A Analysis Of Collagen Filmsmentioning

confidence: 86%

Section: Discussionmentioning

confidence: 99%

Section: Tenascin-c Promoter Activitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Evaluating the performance of fibrillar collagen films formed at polystyrene surfaces as cell culture substrates

et al. 2008

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“…These images consist of A10 rat smooth vascular muscle cells and 3T3 mouse fibroblasts stained with Texas Red cell body stain [11]. For each set, we compared six different images for a total of 96 images.…”

Section: Data Descriptionmentioning

confidence: 99%

A Quality Pre-processor for Biological Cell Images

Peskin¹,

Kafadar

Dima

2009

Advances in Visual Computing

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Abstract. We have developed a method to rapidly test the quality of a biological image, to identify appropriate segmentation methods that will render high quality segmentations for cells within that image. The key contribution is the development of a measure of the clarity of an individual biological cell within an image that can be quickly and directly used to select a segmentation method during a high content screening process. This method is based on the gradient of the pixel intensity field at cell edges and on the distribution of pixel intensities just inside cell edges. We have developed a technique to synthesize biological cell images with varying qualities to create standardized images for testing segmentation methods. Differences in quality indices reflect observed differences in resulting masks of the same cell imaged under a variety of conditions.

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