Vascular endothelial growth factor stimulates the primary to secondary follicle transition in bovine follicles in vitro

Yang, Ming; Fortune, J.E.

doi:10.1002/mrd.20633

Cited by 84 publications

(88 citation statements)

References 31 publications

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“…These results seem to be in agreement with the recent experiments of Yang & Fortune (2007) showing that in Figure 5 A general model that describes the distribution of the follicular subpopulations identified inside each follicular class by using cluster analysis. The subpopulations were represented within each class and arranged on the y-axis by considering the distances between the bifurcation obtained in the dendograms.…”

Section: Discussionsupporting

confidence: 92%

Vascular supply as a discriminating factor for pig preantral follicle selection

Martelli¹,

Bernabò²,

Berardinelli³

et al. 2009

Reproduction

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This research analyses how somatic and vascular compartments change during preantral follicle growth. To address this aim, theca-granulosa (somatic) proliferation indexes (PIs), proportion of proliferating endothelial cells (PE), vascular area (VA) and vascular endothelial growth factor A (VEGFA) expression were simultaneously recorded on single healthy preantral follicles, classified into six different stages on the basis of the diameter and the granulosa layers. An autonomous blood vessel network starts to appear only in class 3. Vascular remodelling requires VEGFA expression, and VEGFA mRNA and VA significantly increase between class 3 and classes 4 and 5 and, further, in class 6. In addition, a positive correlation exists between these parameters in classes 3-5. Despite variation in angiogenesis results from classes 3 to 5, the statistical analysis reveals that the vascular parameters are positively and strictly correlated with somatic PIs. Conversely, class 6, also characterized by higher values of somatic PIs, displays a stable proportion of PEs (y40%) without showing any correlation among the different parameters analysed. To identify follicular subpopulations within different classes, a multivariate hierarchical cluster analysis was performed. This analysis reveals that the majority of classes 3 and 4 are quiescent follicles or structures that grow very slowly. Class 5 represents a transitory category, where half of the follicles maintain a low activity and the remaining express significantly higher levels of granulosa PI and VA. The follicles with this high activity are probably able to reach class 6 becoming dominant structures where somatic and vascular parameters are constantly on high levels and the VA remains the unique differentiating element.

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Section: Discussionsupporting

confidence: 92%

Vascular supply as a discriminating factor for pig preantral follicle selection

Martelli¹,

Bernabò²,

Berardinelli³

et al. 2009

Reproduction

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“…Similar results were reported recently with concentrations of 300-500 ng mL -1 VEGF (Anchordoquy et al 2015). Positive effects of VEGF have also been shown to promote the transition from primary to secondary follicles in the bovine (Yang and Fortune 2007) and to induce follicular growth and increase oocyte diameter in the goat (Bruno et al 2009 Therefore, in the present study we compared VEGF content of porcine COCs from MF and SF, as well as that secreted during IVM. In addition, we evaluated the effects of VEGF supplementation during the first 20 h of IVM on improvements in the meiotic and developmental competence of oocytes derived from SF.…”

Section: Introductionsupporting

confidence: 83%

Presence of vascular endothelial growth factor during the first half of IVM improves the meiotic and developmental competence of porcine oocytes from small follicles

Bui

Nguyễn

Asako

et al. 2017

Reprod. Fertil. Dev.

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The aim of the present study was to investigate the effect of vascular endothelial growth factor (VEGF) on the meiotic and developmental competence of porcine oocytes from small follicles (SF; 0.5-3 mm diameter). When cumulus-oocyte complexes (COCs) from medium-sized follicles (MF; 3-6 mm diameter) and SF were cultured for IVM, the maturation rates were significantly higher for oocytes from MF than SF. Concentrations of VEGF in the medium were significantly higher for COCs cultured from MF than SF. When COCs from SF were exposed to 200 ng mL -1 VEGF during the first 20 h of IVM, the maturation rate improved significantly and was similar to that of oocytes derived from MF. The fertilisability of oocytes was also significantly higher than that of VEGF-free SF controls. Following parthenogenetic activation, the blastocyst formation rate improved significantly when SF COC culture was supplemented with 200 ng mL -1 VEGF, with the rate similar to that of oocytes from MF. The results of the present study indicate that VEGF markedly improves the meiotic and developmental competence of oocytes derived from SF, especially at a concentration of 200 ng mL -1 during the first 20 h of IVM.Additional keyword: pig. T. M. T. Bui et al. Effect of VEGF on IVM of porcine oocytesOvaries contain a large number of small follicles, but the meiotic and developmental competence of oocytes from these follicles is quite low. In the present study we demonstrated that the addition of 200 ng mL -1 vascular endothelial growth factor to the IVM medium increased the maturation rate of porcine oocytes from small follicles and that blastocyst formation following parthenogenetic activation also increased. These findings may contribute to efficient animal production and human assisted reproductive technology.

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“…The likely candidate is VEGFA, which is first detected in the granulosa and theca layers of secondary follicles in cows (Yang & Fortune 2007) while ANGPT and FGF2 do not appear in these cells until the antral stages (van Wezel et al 1995, Hayashi et al 2004. Furthermore, administration of VEGFA stimulated the development of secondary follicles in cows (Yang & Fortune 2007). While VEGF trap administration in primates reduced the endothelial cell area of secondary follicles and inhibited the formation of antral follicles (Wulff et al 2002).…”

Section: Initial Recruitment Of Thecal Vasculaturementioning

confidence: 99%

Angiogenesis and vascular function in the ovary

Robinson

Woad²,

Hammond³

et al. 2009

Reproduction

239

210

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Ovarian function is dependent on the establishment and continual remodelling of a complex vascular system. This enables the follicle and/or corpus luteum (CL) to receive the required supply of nutrients, oxygen and hormonal support as well as facilitating the release of steroids. Moreover, the inhibition of angiogenesis results in the attenuation of follicular growth, disruption of ovulation and drastic effects on the development and function of the CL. It appears that the production and action of vascular endothelial growth factor A (VEGFA) is necessary at all these stages of development. However, the expression of fibroblast growth factor 2 (FGF2) in the cow is more dynamic than that of VEGFA with a dramatic upregulation during the follicular-luteal transition. This upregulation is then likely to initiate intense angiogenesis in the presence of high VEGFA levels. Recently, we have developed a novel ovarian physiological angiogenesis culture system in which highly organised and intricate endothelial cell networks are formed. This system will enable us to elucidate the complex inter-play between FGF2 and VEGFA as well as other angiogenic factors in the regulation of luteal angiogenesis. Furthermore, recent evidence indicates that pericytes might play an active role in driving angiogenesis and highlights the importance of pericyte-endothelial interactions in this process. Finally, the targeted promotion of angiogenesis may lead to the development of novel strategies to alleviate luteal inadequacy and infertility.

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Vascular endothelial growth factor stimulates the primary to secondary follicle transition in bovine follicles in vitro

Cited by 84 publications

References 31 publications

Vascular supply as a discriminating factor for pig preantral follicle selection

Vascular supply as a discriminating factor for pig preantral follicle selection

Presence of vascular endothelial growth factor during the first half of IVM improves the meiotic and developmental competence of porcine oocytes from small follicles

Angiogenesis and vascular function in the ovary

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