Varitation of Stomatal Characteristics in Broad Leaved Species Based on Habitat

Abstract

This research aims to find the habitat-dependent variation of micromorphological characteristics in the leaves of Fraxinus excelsior (European ash), Platanus orientalis (Plane tree) and Tilia tomentosa (Silver lime) collected from 6 different cities of different climates. The leaves were collected in Turkey in the cities of Rize, Samsun, İzmir, Antalya, Sivas, and Ankara. Samsun and Rize fall under the Black Sea climate region, Ankara and Sivas under the continental climate, whereas Antalya and İzmir enjoy a Mediterranean climate. Using scanning electron microscopy (SEM) to highlight the visible morphological characteristics of the plant, we hoped to uncover how stress affects the micro-morphological properties of the plant but does not affect its vital functions. Leaf epidermis images were obtained with SEM and stoma width (μm), length (μm), pore width (μm), pore length (μm), and density (in an area of 1 mm 2 ) measurements were obtained using Image J (https://imagej.net) software. A Duncan test and variance analysis were performed via SPSS® (IBM Analytics) and the habitat-dependent change of these characteristics was evaluated statistically. Accordingly, we determined that the stomatal characteristics varied depending on the habitat, but trees were affected differently by climate conditions. The micromorphological characteristics on the leaves depending on growth conditions were also investigated.

Using Cedrus atlantica’s annual rings as a biomonitor in observing the changes of Ni and Co concentrations in the atmosphere

“…Thus, the linear change in Ni concentration by the traffic volume and the lower level of relationship between Co concentration change and traffic volume can be considered as a result of elements, plant structure, and interaction between them because many factors affect the intake and accumulation of heavy metals in plants. Besides the factors related to plant structure and environments such as plant species, precipitation, moist level, plant habitus, and organ structure, the type of heavy metal and its interaction with the plant are also important factors affecting the heavy metal accumulation in the organs of the plant (Cetin et al 2018a(Cetin et al , 2018bYigit et al 2019;Aricak et al 2020;Sevik et al 2020c).…”

The effect of shadow conditions on stomatal characters of several plants used in landscape design

“…The scanning electron microscope, which was used in taking the stoma images within the scope of the present study, provides a high level of magnification thanks to high-resolution imaging techniques. For this reason, it is possible to obtain morphological, structural, and elemental data from the plants at a high zoom level [9,24]. The data obtained can be used in various areas such as determining the stress level of plants, genetic variation studies, and determining the most suitable plant growth conditions [8].…”

“…Besides that, it is known that genetic variations may be seen within the same species due to differences in growing conditions and origins [31][32][33]. Previous studies reported that stomatal characteristics might significantly vary between the individuals, which have been grown in the same environment, from the same species [9].…”

“…Stomas are the structures controlling the intake-output of CO2 and water vapor in leaves and having vital importance for the plant [9]. If the factors affecting the development of stomatal characteristics can be determined, then this information can be used in many fields such as examining the stress status of plants, as well as genetic variation studies.…”

Rangeland rehabilitation using micro-catchments and native species in Turkana County, Kenya

“…There are many factors such as precipitation, temperature, stress factors, light, air pollution, and soil structure which affect the morphological characteristics and development of plants (Cetin et al, 2018a,b;Aricak et al, 2020). In addition to ecological conditions, the studies conducted reveal that the factors such as pruning, hormone applications, spraying, shading, and fertilizing factors are also effective on plant morphological characteristics and development (Guney et al, 2016;Sevik and Cetin, 2016;Yigit et al, 2019). However, the reactions given to these conditions by plants are also shaped under the influence of genetic structure.…”