Variation outside variable segments of the major outer membrane protein distinguishes trachoma from urogenital isolates of the same serovar of Chlamydia trachomatis.

Frost, Éric; Deslandes, Sylvie; Gendron, D; Bourgaux-Ramoisy, Danielle; Bourgaux, Pierre

doi:10.1136/sti.71.1.18

Cited by 19 publications

(25 citation statements)

References 17 publications

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“…1-3). This mutational pattern has also been observed for variants of other serovars (1,2,(11)(12)(13)(14)(15)(16)(17). Interestingly, some of the mutations contained in the E variants resulted in the same amino acid found in closely related serovars: Leu 157 Phe for variants E/1, E/2, and E/48, and serovars D and L1 ( Fig.…”

Section: Discussionmentioning

confidence: 68%

“…Many of these variants contain amino acid changes which cannot be identified by serotyping (18,19). Among STD populations, a relatively high number of variants have been described among D and F serovars, yet relatively few have been identified for serovar E (1,2,(11)(12)(13). This may reflect the fact that only VS1, 2, and 4 sequences have been used for omp1 genotyping, not VS3 or constant regions.…”

Section: Introductionmentioning

confidence: 98%

“…This may reflect the fact that only VS1, 2, and 4 sequences have been used for omp1 genotyping, not VS3 or constant regions. In a study by Frost et al (13), nucleotide sequence variation in constant regions was able to differentiate ocular (trachoma) from genital samples of the same serotype. It is possible that the constant regions of E serovars may also contain sequence variation.…”

Section: Introductionmentioning

confidence: 99%

“…This research has revealed an array of genotypic variation within each of the known serovars (1,2,(11)(12)(13)(14)(15)(16)(17). Many of these variants contain amino acid changes which cannot be identified by serotyping (18,19).…”

Section: Introductionmentioning

confidence: 99%

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Molecular and mutation trends analyses of omp1 alleles for serovar E of Chlamydia trachomatis. Implications for the immunopathogenesis of disease.

Dean¹,

Millman²

1997

J. Clin. Invest.

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Serovars E, F, and D are the most prevalent Chlamydia trachomatis strains worldwide. This prevalence may relate to epitopes that enhance infectivity and transmission. There are numerous major outer membrane protein (MOMP) gene ( omp1 ) variants described for D and F but few for E. However, omp1 constant regions are rarely sequenced, yet, they may contain mutations that affect the structure/function relationship of the protein. Further, differentiating variants that occur as a result of selection from variants that contain random mutations without biologic impact is difficult. We investigated 67 urogenital E serovars and found 11 (16%) variants which contained 16 (53%) nonconservative amino acid changes. Using signature-pattern analysis, 57 amino acids throughout MOMP differentiated the E sequence set from the non-E sequence set, thus defining E strains. Four E variants did not match this signature-pattern, and, by phenetic analyses, formed new phylogenetic branches, suggesting that they may be biologically distinct variants. Our analyses offer for the first time a unique approach for identifying variants that may occur from selection and may affect infectivity and transmission. Understanding the mutation trends, phylogeny, and molecular epidemiology of E variants is essential for designing public health control interventions and a vaccine. ( J. Clin. Invest. 1997. 99:475-483.)

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Section: Discussionmentioning

confidence: 68%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Molecular and mutation trends analyses of omp1 alleles for serovar E of Chlamydia trachomatis. Implications for the immunopathogenesis of disease.

Dean¹,

Millman²

1997

J. Clin. Invest.

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“…An interesting group of clinical isolates among the samples we tested were the genital samples ompA sequenced that typed as serovar B. Although serovar B is most commonly associated with ocular trachoma (2,4,5), it has also been isolated from patients with urogenital disease (6)(7)(8)36). Since these genital Bs represent an unusual example of a crossover serovar, we decided to characterize them in more detail.…”

Section: Figurementioning

confidence: 99%

Polymorphisms in Chlamydia trachomatis tryptophan synthase genes differentiate between genital and ocular isolates

Wood¹,

Crane²,

Bailey³

et al. 2003

J. Clin. Invest.

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We previously reported that laboratory reference strains of Chlamydia trachomatis differing in infection organotropism correlated with inactivating mutations in the pathogen’s tryptophan synthase (trpBA) genes. Here, we have applied functional genomics to extend this work and find that the paradigm established for reference serovars also applies to clinical isolates — specifically, all ocular trachoma isolates tested have inactivating mutations in the synthase, whereas all genital isolates encode a functional enzyme. Moreover, functional enzyme activity was directly correlated to IFN-γ resistance through an indole rescue mechanism. Hence, a strong selective pressure exists for genital strains to maintain a functional synthase capable of using indole for tryptophan biosynthesis. The fact that ocular serovars (serovar B) isolated from the genital tract were found to possess a functional synthase provided further persuasive evidence of this association. These results argue that there is an important host-parasite relationship between chlamydial genital strains and the human host that determines organotropism of infection and the pathophysiology of disease. We speculate that this relationship involves the production of indole by components of the vaginal microbial flora, allowing chlamydiae to escape IFN-γ–mediated eradication and thus establish persistent infection

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Comparison of reverse hybridization and ompA sequencing methods applied on Chlamydia trachomatis strains from Tunisia

et al. 2017

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Two techniques based on ompA amplification of Chlamydia trachomatis were compared, being reverse hybridization (RHM) and ompA sequencing (OSA), to investigate the concordance between them and to study the epidemiological relevance of each method. In addition, phylogenetic analysis was performed on the ompA sequences. One hundred and seven C. trachomatis positive samples from Tunisian patients and female sex workers were analyzed using both the RHM and ompA sequencing. The overall genovar distribution obtained with both techniques was very similar. The RHM identified nine genovars, being B, D, E, F, G, H, I, J and K, where B, I, J, and K were only found in mixed infections versus 7 types for the OSA being D, E, F, G, H, I, and K. The agreement between both typing techniques was 87.8%. Both methods showed that genovar E was the most predominant type. In 24.3% of the analyzed samples, mixed infections were detected. In 96.1% of these, the genovar identified by OSA was also detected using the RHM. OmpA sequencing allowed determination of six genovar types that could not be typed using RHM. The analyses of ompA nucleotide variation in the 107 clinical specimens detected ompA genovar variants with distinct ompA mutational patterns for types D2, G1, G2, and H1. In conclusion, RHM and OSA showed a high agreement in C. trachomatis genotyping results with each having their specific benefits.

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Variation outside variable segments of the major outer membrane protein distinguishes trachoma from urogenital isolates of the same serovar of Chlamydia trachomatis.

Cited by 19 publications

References 17 publications

Molecular and mutation trends analyses of omp1 alleles for serovar E of Chlamydia trachomatis. Implications for the immunopathogenesis of disease.

Molecular and mutation trends analyses of omp1 alleles for serovar E of Chlamydia trachomatis. Implications for the immunopathogenesis of disease.

Polymorphisms in Chlamydia trachomatis tryptophan synthase genes differentiate between genital and ocular isolates

Comparison of reverse hybridization and ompA sequencing methods applied on Chlamydia trachomatis strains from Tunisia

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