Variation of 2,4-dichlorophenoxyacetic acid (2,4-D) concentration on kaffir lime callus growth as raw material for cell suspension

Damayanti, Frisca; Indrianto, Ari; Sasongko, Aries Bagus; Fajarina, Sudewi; Prabowo, Beni Hendro; Iskandar, Agnes; Hidayati, Lisna; Tunjung, Woro Anindito Sri

doi:10.1063/5.0016420

Cited by 6 publications

(3 citation statements)

References 6 publications

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“…Furthermore, the use of 2,4-D by itself produced a callus with a friable structure (Amoo & Aysire, 2005). Our study also showed that the addition of 2,4-D without BAP will produce a very friable callus, which is suitable as raw material for cell suspension (Damayanti et al, 2020). Hence, in this study, we used a basal medium with three different concentrations and combinations of growth regulators, namely, 2,4-D:BAP (2:0 ppm), 2,4-D:BAP (5:0 ppm), and 2,4-D:BAP (1:0.5 ppm).…”

Section: Introductionsupporting

confidence: 55%

“…This basal medium was added with a combination of various concentrations of 2,4-Dichlorophenoxyacetic (Sigma-Aldrich):BAP (Benzylaminopurine) (Sigma-Aldrich) hormones. The concentration of growth regulators was based on previous studies, in which we tried several concentrations of 2,4-D and BAP (Tunjung et al, 2020;Tunjung et al, 2018) and several concentrations of 2,4-D (Damayanti et al, 2020). Hence, in this study, we focused only on three concentrations of 2,4-D: BAP namely 1:0.5, 2:0 and 5:0 ppm.…”

Section: Callus Inductionmentioning

confidence: 99%

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Effect of 2,4-D and BAP on Morphological Characters and Genetic Stability of Kaffir Lime (Citrus hystrix DC.) Callus Cultures Among Generations

Tunjung

Widyasari

Iskandar

et al. 2021

CMUJNS

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Kaffir lime has medicinal properties for the treatment of many diseases. We used in vitro culture techniques to maintain quality and increase the production of active compounds in kaffir lime. The objective of this study is to determine the phenotype and genotype of kaffir lime callus cultures grown on media with the addition of 2,4-Dichlorophenoxyacetic (2,4-D) and Benzylaminopurine (BAP) on three generations (G0, G1, and G2). The callus was induced and subcultured on Murashige and Skoog medium added with 2,4-D: BAP at concentrations of 1:0.5; 2:0 and 5:0. Results showed no significant difference in callus initiation time in all treatment groups. Morphological characteristics, including color, texture, and biomass, varied among growth regulator concentrations and generation level. A high level of callus generation corresponds to a more friable texture and a more yellowish callus. Callus grown with the addition of 2,4-D and BAP 2:0 and 5:0 showed a more friable structure and yellowish color than 1:0.5. Growth regulator concentrations in each generation did not affect the callus growth curves, but the length of each phase between generations was different. The exponential phases of G1 and G2 were faster than that of G0. Despite slight differences in phenotype, the DNA profiles of callus suggest the same pattern between treatment groups, thereby indicating that our kaffir lime callus has genetic stability and that the callus can be used as raw material for medicinal purposes. Keywords: ISSR markers, Kaffir lime callus, Generations, Genotype, Growth factor, Phenotype

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Section: Introductionsupporting

confidence: 55%

Section: Callus Inductionmentioning

confidence: 99%

Effect of 2,4-D and BAP on Morphological Characters and Genetic Stability of Kaffir Lime (Citrus hystrix DC.) Callus Cultures Among Generations

Tunjung

Widyasari

Iskandar

et al. 2021

CMUJNS

Self Cite

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“…The predominant meristematic nature of callus cells (Coimbra et al, 2019) maximises cell division during the exponential phase (Santos et al, 2017), and increases biomass. Furthermore, in addition to the continuous production of primary metabolites (Damayanti et al, 2020), the diameter and volume of the callus cells increase due to cell enlargement (Bhatia, 2015) during the exponential phase which resulted in an increment of biomass. However, in terms of DW, the growth of Callus 8 from day 45 to 60 was denoted insignificant (0.31 to 0.33g) indicating a plateau condition.…”

Section: Establishment Of the Callus Growth Curvementioning

confidence: 99%

Picloram enhanced the callus induction, growth kinetics, antioxidant potentials, and secondary metabolites production of Zingiber officinale var. rubrum callus cultures

Gnasekaran

Rahman

Chew

et al. 2023

Preprint

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Plant cells are driven by types and concentrations of plant growth regulators to produce callus mass containing bioactive compounds. This study aimed to induce callus and to observe the histological, phytochemicals, and antioxidant basis of the callus. An efficient callus induction protocol was developed using picloram for Malaysian red ginger, Zingiber officinale var. rubrum. The effect of auxinic picloram herbicide was studied using six different concentrations (0, 0.5, 1, 2, 4, and 8 mg/L) on various explants (leaf sheath, leaf, root) to optimise the callus induction. The induced callus was studied for growth kinetics, anatomical features, antioxidant capacity, and phytochemical content. The highest callogenesis frequency (93.75%) and biomass accumulation (3.68 g) were observed on leaf sheath explant cultured on ½ strength Murashige and Skoog (MS) medium supplemented with 8 mg/L which also requires earlier subculture duration (45 days post-inoculation) in comparison to lower concentrations. Morphological investigation through histological procedure demonstrated friable and non-embryogenic characteristics of the primary and subcultured callus. Cultivated leaf sheath (CLS) methanolic extract showed the highest total phenolic (191.26 mg GAE/g dry extract) and flavonoid (4.54 mg QE/g dry extract) contents contributing to antioxidant activity with an estimated EC50 of 0.208 mg/mL. Although comparatively lower than CLS extract, callus extracts showed higher antioxidant activity and significantly lower EC50 values than in vitro leaf sheath extract. 4H-Pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl-, phenol, and phenolic glucoside were only present in callus cultures while methyl esters, fatty acids, and phytosterols could be obtained from leaf sheath and callus extracts. In conclusion, the callus culture of Z. officinale var. rubrum is a potential renewable source of bioactive phytochemical compounds and can be employed for biotechnological practices such as elicitor-induced accumulation of secondary metabolites and genetic modification.

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Picloram enhanced the callus induction, growth kinetics, antioxidant potentials, and secondary metabolites production of Zingiber officinale var. rubrum callus cultures

Gnasekaran,

Rahman,

Chew

et al. 2023

Plant Cell Tiss Organ Cult

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Variation of 2,4-dichlorophenoxyacetic acid (2,4-D) concentration on kaffir lime callus growth as raw material for cell suspension

Cited by 6 publications

References 6 publications

Effect of 2,4-D and BAP on Morphological Characters and Genetic Stability of Kaffir Lime (Citrus hystrix DC.) Callus Cultures Among Generations

Effect of 2,4-D and BAP on Morphological Characters and Genetic Stability of Kaffir Lime (Citrus hystrix DC.) Callus Cultures Among Generations

Picloram enhanced the callus induction, growth kinetics, antioxidant potentials, and secondary metabolites production of Zingiber officinale var. rubrum callus cultures

Picloram enhanced the callus induction, growth kinetics, antioxidant potentials, and secondary metabolites production of Zingiber officinale var. rubrum callus cultures

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