Wood specific gravity (WSG) is an important parameter in allometric equations for accurate estimation of C-sequestration and other functional properties of a tree. However, WSG of many tree species especially of arid and semi-arid regions is poorly reported. Further, identifying indirect methods for determination of stem WSG from branches may be rapid and relatively easy. The present study determined WSG of stem and branches of 21 tree species in the hot semi-arid region of Western India. Three individual trees from each species were randomly selected and sampled for determination of WSG of stem, primary and secondary branch. WSG varied significantly among the species (F = 42.83, P < 0.001) and sampling locations (stem and branches) (F = 29.43, P < 0.001). In stem (at DBH), it ranged from 0.42 0.04 to 0.74 0.03 among the species while within an individual tree it varied in order of stem > primary branch > secondary branch in most species. WSG of stem and branches showed linear relationship and branches were found a good predictor of stem WSG (R 2 > 0.83).Keywords: Arid region, branch, tree biomass, wood specific gravity.INCREASING level of greenhouse gases (GHGs) especially CO 2 is a major cause of global warming and climate change 1 . Tree plantation provides a potential mitigation option for arresting climate change by absorbing and converting CO 2 into biomass through photosynthesis. However, direct method of estimating C-sequestration potential of a tree species is destructive, requiring harvesting of trees for determination of biomass and carbon. Cutting of trees in many countries is an environmental issue and legally not allowed especially in areas with scarce vegetation like semi-arid and arid zones. Allometric equations and models provide better option for nondestructive, indirect methods of tree biomass determination. These allometric equations have been reported to be more accurate when wood specific gravity (WSG) is included into the equation 2 . The wood carbon content is also highly correlated with WSG 3 . Thus, estimation of tree biomass and C-content depends critically on reliable information on WSG of trees. Research is being carried out to quantify potential of tree based C-sequestration in arid and semi-arid regions 4 . However, data on WSG of tree species found and grown in arid and semi-arid environment is scarce, leading to the choice of destructive methods of estimation. WSG of a tree varies with species and climatic condition 2,5,6 . The documentation of WSG of hot semi-arid zone of India is required for enhancing accuracy of allometric equation for better prediction of above ground biomass without harvesting of the tree. Further, variation in WSG has also been reported within the main stem and branches of a tree [7][8][9] . Determination of WSG of branches and developing relationship is useful and avoids the tedious process of wood core sampling and tree harvesting. With this background, the present study was undertaken to determine WSG of prominent tree species of hot semi-arid regio...