Variation in sperm displacement and its association with accessory gland protein loci in Drosophila melanogaster.

Clark, Andrew G.; Aguadé, Montserrat; Prout, Timothy; Harshman, Lawrence G.; Langley, Charles H.

doi:10.1093/genetics/139.1.189

Cited by 337 publications

(84 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous studies that measured the proportion of progeny sired by alternative males in double mating experiments have suggested that the competitive ability of sperm varies among male genotypes [8]. In particular, bw D males have been shown to be poor sperm removers on the basis of the proportion of progeny they sire as second males (their P2 scores) [10].…”

Section: Resultsmentioning

confidence: 99%

“…Scores of sperm competition were obtained by setting up matings between 4-6-day-old virgin B3-09 females to same-aged dj-GFP males (which bear the CyO marker) followed by males from lines B3-09 or bw D . Sperm competition (P2) was measured from progeny counts [8] obtained from these matings. Progeny were scored for wing phenotype (wild type if sired by bw D or B3-09 males and curly if sired by dj-GFP males) about 15 days after oviposition began.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Direct visualization of sperm competition and sperm storage in Drosophila

Civetta

1999

Current Biology

View full text Add to dashboard Cite

Drosophila females engage in multiple matings [1] [2] [3] [4] even though they can store sperm in specialized organs for most of their life [5]. The existence of sperm competition in Drosophila has been inferred from the proportion of progeny sired by the second male in double-mating experiments [6] [7] [8]. Investigators have used this approach to quantify genetic variation underlying sperm competition [8] [9] [10], to elucidate its genetic basis [11], to identify the dependence of different male competitive ability on the genotype of the females with which they mate [12] and to discern the potential role of sperm competition in species isolation [13] [14]. This approach assumes that the sperm from two males stored in a female compete to fertilize the eggs. The mechanism by which sperm competition is accomplished is still unknown, however. Here, fluorescence microscopy, cytometry, and differently labeled sperm were used to analyze the fate of sperm inside the female's sperm storage organs, to quantify sperm competition, and to assess how closely paternity success corresponds to the appearance and location of the sperm. The results show that the first male's sperm is retained for a shortened period if the female remates, and that the second males that sire more progeny either induce females to store and use more of their sperm or strongly displace resident sperm.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Direct visualization of sperm competition and sperm storage in Drosophila

Civetta

1999

Current Biology

View full text Add to dashboard Cite

show abstract

“…For example, females mated to transgenic males that lack specific seminal fluid proteins, show differences in post-mating transcript abundances, as compared to females mated to wildtype males with a full complement of seminal fluid proteins (McGraw et al 2004(McGraw et al , 2008Domanitskaya et al 2007;Gioti et al 2012). Additionally, polymorphisms in genes encoding seminal fluid proteins impact female post-mating responses and the male's reproductive success (Clark et al 1995;Prout and Clark 1996;Hughes 1997;Clark et al 2000;Fiumera et al 2005Fiumera et al , 2006; Greenspan and Clark 2011;Lüpold et al 2012;Zhang et al 2013). Thus, male seminal fluid proteins represent a major molecular component in the reproductive interactions that affect post-mating phenotypes in D. melanogaster.…”

mentioning

confidence: 99%

Roles of Female and Male Genotype in Post-Mating Responses in Drosophila melanogaster

Delbare

Chow

Wolfner

et al. 2017

Journal of Heredity

View full text Add to dashboard Cite

Mating induces a multitude of changes in female behavior, physiology, and gene expression. Interactions between female and male genotype lead to variation in post-mating phenotypes and reproductive success. So far, few female molecules responsible for these interactions have been identified. Here, we used Drosophila melanogaster from 5 geographically dispersed populations to investigate such female × male genotypic interactions at the female transcriptomic and phenotypic levels. Females from each line were singly-mated to males from the same 5 lines, for a total of 25 combinations. Reproductive output and refractoriness to re-mating were assayed in females from the 25 mating combinations. Female × male genotypic interactions resulted in significant differences in these post-mating phenotypes. To assess whether female × male genotypic interactions affect the female post-mating transcriptome, next-generation RNA sequencing was performed on virgin and mated females at 5 to 6 h post-mating. Seventy-seven genes showed strong variation in mating-induced expression changes in a female × male genotype-dependent manner. These genes were enriched for immune response and odorant-binding functions, and for expression exclusively in the head. Strikingly, variation in post-mating transcript levels of a gene encoding a spermathecal endopeptidase was correlated with short-term egg production. The transcriptional variation found in specific functional classes of genes might be a read-out of female × male compatibility at a molecular level. Understanding the roles these genes play in the female post-mating response will be crucial to better understand the evolution of post-mating responses and related conflicts between the sexes.

show abstract

“…Embryos did not die due to excessive polyspermy, which is thought to be a risk for females copulating with multiple males with highly competitive sperm [119,120] (although some degree of polyspermy may be beneficial in birds [121,122]). The same sperm trait was positively associated both with a "defensive" role in fertilizing within-pair eggs and an "offensive" role in fertilizing extra-pair eggs [46,123], and a single sperm trait drove fertilization success [124]. All of these complexities are likely to weaken or obscure the relationship between sperm traits and fertilization success, because variation in fertilization success is explained by the other factors [14,88,89] or because selection on the sperm trait is non-linear.…”

Section: Simplifying Assumptions In the Simulationmentioning

confidence: 99%

Measuring Pre- and Post-Copulatory Sexual Selection and Their Interaction in Socially Monogamous Species with Extra-Pair Paternity

Cramer

2021

Cells

View full text Add to dashboard Cite

When females copulate with multiple males, pre- and post-copulatory sexual selection may interact synergistically or in opposition. Studying this interaction in wild populations is complex and potentially biased, because copulation and fertilization success are often inferred from offspring parentage rather than being directly measured. Here, I simulated 15 species of socially monogamous birds with varying levels of extra-pair paternity, where I could independently cause a male secondary sexual trait to improve copulation success, and a sperm trait to improve fertilization success. By varying the degree of correlation between the male and sperm traits, I show that several common statistical approaches, including univariate selection gradients and paired t-tests comparing extra-pair males to the within-pair males they cuckolded, can give highly biased results for sperm traits. These tests should therefore be avoided for sperm traits in socially monogamous species with extra-pair paternity, unless the sperm trait is known to be uncorrelated with male trait(s) impacting copulation success. In contrast, multivariate selection analysis and a regression of the proportion of extra-pair brood(s) sired on the sperm trait of the extra-pair male (including only broods where the male sired ≥1 extra-pair offspring) were unbiased, and appear likely to be unbiased under a broad range of conditions for this mating system. In addition, I investigated whether the occurrence of pre-copulatory selection impacted the strength of post-copulatory selection, and vice versa. I found no evidence of an interaction under the conditions simulated, where the male trait impacted only copulation success and the sperm trait impacted only fertilization success. Instead, direct selection on each trait was independent of whether the other trait was under selection. Although pre- and post-copulatory selection strength was independent, selection on the two traits was positively correlated across species because selection on both traits increased with the frequency of extra-pair copulations in these socially monogamous species.

show abstract

Variation in sperm displacement and its association with accessory gland protein loci in Drosophila melanogaster.

Cited by 337 publications

References 27 publications

Direct visualization of sperm competition and sperm storage in Drosophila

Direct visualization of sperm competition and sperm storage in Drosophila

Roles of Female and Male Genotype in Post-Mating Responses in Drosophila melanogaster

Measuring Pre- and Post-Copulatory Sexual Selection and Their Interaction in Socially Monogamous Species with Extra-Pair Paternity

Contact Info

Product

Resources

About