Variant Lineages of Human Papillomavirus Type 18 in Northeast China Populations Characterized by Sequence Analysis of E6, E7, and L1 Regions

Sun, Zhengrong; Liu, Jianhua; Wang, Guili; Zhou, Weiqiang; Liu, Chao; Ruan, Qiang

doi:10.1097/igc.0b013e318253a994

Cited by 9 publications

(10 citation statements)

References 27 publications

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“…Our LCR/E6 classification fits with other HPV18 sequences reported in the literature (10,12,(40)(41)(42)(43)(44)(45)(46)(47)(48)(49)(50) and is consistent with lineage definitions based on the gold standard approach of HPV18 whole-genome sequencing (13). Although based on LCR and E6 only, our analysis included many novel variants, particularly among previously underrepresented A5 and B (sub)lineages, which would warrant sequencing the whole genomes in order to strengthen the full picture of HPV18 genetic evolution.…”

Section: Discussionsupporting

confidence: 48%

Human Papillomavirus 18 Genetic Variation and Cervical Cancer Risk Worldwide

Chen

Gheit

Franceschi

et al. 2015

J Virol

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Human papillomavirus 18 (HPV18) is the second most carcinogenic HPV type, after HPV16, and it accounts for approximately 12% of squamous cell carcinoma (SCC) as well as 37% of adenocarcinoma (ADC) of the cervix worldwide. We aimed to evaluate the worldwide diversity and carcinogenicity of HPV18 genetic variants by sequencing the entire long control region ( In region-stratified analyses, there were no significant differences in the distribution of HPV18 variant lineages between cervical cancer cases and controls or between ADC and SCC. In conclusion, our findings do not support the role of HPV18 (sub)lineages for discriminating cancer risk or explaining why HPV18 is more strongly linked with ADC than SCC. IMPORTANCEThis is the largest and most geographically/ethnically diverse study of the genetic variation of HPV18 to date, providing a comprehensive reference for phylogenetic classification of HPV18 sublineages for epidemiological and biological studies.

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Section: Discussionsupporting

confidence: 48%

Human Papillomavirus 18 Genetic Variation and Cervical Cancer Risk Worldwide

Chen

Gheit

Franceschi

et al. 2015

J Virol

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“…Over 200 HPV types have been identified based on L1 sequences. HPV18 variants were originally grouped into European (E), Asian-Amerindian (AA) or African (AFR) lineages according to E6-E7, L1, and/or LCR sequences [10][11][12][13][14]. This classification has been superseded by a whole viral genome sequencing approach that has defined three major lineages (A, B, and C) and additional sublineages (A1 to A5 and B1 to B3) [15] that can be translated from the historical nomenclature (A1 and A2 are AA, A3 to A5 are E and B/C are AFR) [16,17].…”

Section: Introductionmentioning

confidence: 99%

Fifteen new nucleotide substitutions in variants of human papillomavirus 18 in Korea

Kim

Park

Kim

et al. 2020

Virol J

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High-risk human papillomavirus (HPV) infection is an essential factor for the development of cervical cancer. HPV18 is the second most common carcinogenic HPV type following HPV16, but the lineages of HPV18 have been less well studied than those of HPV 16. The purpose of this study was to analyze the nucleotide variants in the E6, E7, and L1 genes of HPV18, to assess the prevalence of HPV18 variants in Korea and to explore the relationship between HPV18 genetic variants and the risk for cervical cancer. A total of 170 DNA samples from HPV18-positive cervical specimens were collected from women admitted to a secondary referral hospital located in Seoul. Among them, the lineages of the 97 samples could be successfully determined by historical nomenclature. All the studied HPV 18 variants were lineage A. Sublineages A1 and A4 comprised 91.7% (89/97) and 1.0% (1/97), respectively. Sublineages other than A1 or A4 comprised 7.2% (7/97). We identified 15 new nucleotide substitutions among 44 nucleotide substitutions: C158T,

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“…Speci c variations of HPV 18 may present high oncogenic potential and in uence the variation of distribution of cervical cancer in different regions (20). However, a study by Villa et al (2000) indicated that infection by non-European variants presents a greater chance of persistence of infection and association with pre-invasive lesions (20,22). Thus, more research is needed to understand which factors may be associated with virus lineages in the persistence and progression of cervical lesions.…”

Section: Discussionmentioning

confidence: 99%

HPV 18 Variants in Women with Cervical Cancer in Northeast Brasil

Santos¹,

Cunha²,

Batista³

et al. 2020

Preprint

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Abstract Background: Human Papillomavirus (HPV) is the main etiological factor for the development of cervical cancer. HPV 18 is the second most frequent, accounting for up to 65% of all cases. HPV intratypic variation may influence the potential for progression to invasive cancer.The aim of this study was to evaluate the prevalence of human papillomavirus 18 intratypic variants in cervical cancer samples from women in the Northeast of Maranhão. Methods: The study was composed of 118 women over 18 years of age with a diagnosis of cervical cancer. Tumor fragments were collected and subjected to DNA extraction and Polymerase Chain Reaction (PCR) for HPV detection using the PGMY09/11 and GP+5/6 primers. Positive samples were submitted to automated sequencing for viral genotyping. To determine the HPV 18 lineages, positive samples were submitted to PCR, using specific primers to amplify the LCR and E6 regions of HPV 18 virus. Results: HPV was present in 88 women (73.3%), and HPV 16 was the most prevalent (48/54%), followed by HPV 18 (12/13.6%). Histologically, squamous cell carcinoma was predominant (79.1%). Among the HPV 18 variants identified, 10 (80%) belonged to lineage A, and sublineages A1, A2, A3, and A4. HPV 18 B variant (2/29%) was also detected, with the sublineages B1 and B2. In this study, the C variant was not found. There was no statistically significant association between the HPV 18 lineages found and sociodemographic and lifestyle variables (p > 0.05). Conclusions: Women with cervical cancer are associated with a higher frequency of HPV 16 and 18 in the Northeast region of Brazil, with a high prevalence of the lineage A among women with HPV 18.

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Variant Lineages of Human Papillomavirus Type 18 in Northeast China Populations Characterized by Sequence Analysis of E6, E7, and L1 Regions

Cited by 9 publications

References 27 publications

Human Papillomavirus 18 Genetic Variation and Cervical Cancer Risk Worldwide

Human Papillomavirus 18 Genetic Variation and Cervical Cancer Risk Worldwide

Fifteen new nucleotide substitutions in variants of human papillomavirus 18 in Korea

HPV 18 Variants in Women with Cervical Cancer in Northeast Brasil

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