Variability of the Microbial Environment of Rotifer Brahionis plicatilis and Avtemia Production Systems

Abstract

Samplings were organized in three marine fish hatcheries. Samples were taken at different steps during the production of algae, rotifers and Artemia. There were 233 dominant colony types isolated for further characterization. The isolates were compared to each other and to reference strains of Phorobacrerium, Yibrio and Alreromonas, using cellular fatty acid fingerprints. Fatty acid profiles of the isolates were grouped using principal component analysis. The isolates fell into at least ten major groups, of which two correspond to the genera Vibrio and to Alteromonas, respectively. Some groups seem to be restricted to a particular type of live food or isolation site. Although 43% of the isolates belong to Vibrio, only a minority of them could be readily assigned to known species.live feed with pathological conditions in fish larvae are presently underway.

Biodiversity of Vibrios

“…Several cultivation-dependent and independent studies have showed that vibrios appear at particularly high densities in and/or on marine organisms, e.g., corals (331), fish (6,146,179,325), molluscs (345), seagrass, sponges, shrimp (133,(404)(405)(406) and zooplankton (163,194,365,408,409).…”

Bacteria and hatching percentage of cysts of Artemia franciscana Kellogg, 1906, from four natural populations in Mexico

“…Bacterial infections, especially those generated by the genus Vibrio, create serious mortality problems in the culture of larval stages. It is not possible to develop these cultures in sterile media, but the potential sources of contamination can be controlled by treating the water by filtration or UV irradiation (Liltved et al, 1995) and, in the larviculture of fish and crustaceans, by improving the sanitary quality of the Artemia nauplii used as food because they can become an important vector for the transmission of Vibrio and other pathogenic bacteria (Verdonck et al, 1994;Lizárraga-Partida et al, 1997). Due to the lack of information regarding the bacterial flora associated with the Mexican populations of Artemia, nauplii obtained from cysts from several sites in Mexico were analyzed in this study.…”

“…Las infecciones bacterianas, especialmente las generadas por el género Vibrio, causan grandes problemas de mortalidad en la etapa de larvicultura. No es posible desarrollar estos cultivos en medios estériles, pero se pueden controlar las fuentes potenciales de contaminación tratando el agua por filtración y por luz ultravioleta (Liltved et al, 1995) y, en la larvicultura de peces y crustáceos, mejorando la calidad sanitaria de los nauplios de Artemia usados como alimento debido a que pueden constituirse en un vector importante para la transmisión de Vibrio y otras bacterias patógenas (Verdonck et al, 1994;Lizárraga-Partida et al, 1997).…”

Use of commercial probiotics for the improvement of water quality and rotifer density in outdoor mass culture tanks

“…Many studies were carried out on the mass culture of rotifers with different microalgal diets and at different temperatures and salinity conditions (Fielder et al, 2000;Hotos, 2002Hotos, , 2003Savas and Guclu, 2006;Kostopoulou and Vadstein, 2007;Jabeur et al, 2013;Abou-Shanab et al, 2016) in the rotifer tanks. Several researchers reported variations in rotifer density due to Vibrio infections which is a major constraint for high density production of rotifers (Skjermo and Vadstein, 1993;Verdonck et al, 1994Verdonck et al, , 1997Gomez-Gil et al, 2000;Rombaut et al, 2001;Prol-Garcia et al, 2010, Jayasree et al, 2016. Use of antibiotics in culture systems will lead to the development of antibiotic resistant strains of pathogenic bacteria and will also hinder the growth of cultured organisms.…”