Variability of miRNA expression during the differentiation of human embryonic stem cells into retinal pigment epithelial cells

Yuan, Zhixiang; Ding, Suping; Yan, Mingli; Zhu, Xiao; Liu, Huan; Tan, Suxu; Jin, Yuanchang; Sun, Yuandong; Li, Yufeng; Huang, Ting

doi:10.1016/j.gene.2015.05.060

Cited by 13 publications

(11 citation statements)

References 82 publications

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“…MiRNA-based differentiation is similarly known as a promising alternative technique that does not involve integration into the genome [11]. MiRNAs can also repress mRNA translation or lead to target mRNA degradation via complementarity binding to the 3′-UTR of target mRNAs [12]. MiRNAs are also involved in numerous normal and pathological cellular and physiological processes through regulating the expression of their target genes [12].…”

Section: Introductionmentioning

confidence: 99%

“…MiRNAs can also repress mRNA translation or lead to target mRNA degradation via complementarity binding to the 3′-UTR of target mRNAs [12]. MiRNAs are also involved in numerous normal and pathological cellular and physiological processes through regulating the expression of their target genes [12]. Moreover, miRNAs play an important role in maturation and functioning of the mammalian retinas [13].…”

Section: Introductionmentioning

confidence: 99%

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Overexpression of MiR-183/96/182 Triggers Retina-Like Fate in Human Bone Marrow-Derived Mesenchymal Stem Cells (hBMSCs) in Culture

Mahmoudian-Sani

Forouzanfar

Asgharzade

et al. 2019

Journal of Ophthalmology

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Retinal degeneration is considered as a condition ensued by different blinding disorders such as retinitis pigmentosa, age-related macular degeneration, and diabetic retinopathy, which can cause loss of photoreceptor cells and also lead to significant vision deficiencies. Although there is no efficient treatment in this domain, transplantation of stem cells has been regarded as a therapeutic approach for retinal degeneration. Thus, the purpose of this study was to analyze the potential of human bone marrow-derived mesenchymal stem cells (hBMSCs) to differentiate into photoreceptor cells via transfection of microRNA (miRNA) in vitro for regenerative medicine purposes. To this end, miR-183/96/182 cluster was transfected into hBMSCs; then, qRT-PCR was performed to measure the expression levels of miR-183/96/182 cluster and some retina-specific neuronal genes such as OTX2, NRL, PKCα, and recoverin. CRX and rhodopsin (RHO) levels were also measured through qRT-PCR and immunocytochemistry, and subsequently, cellular change morphology was detected. The findings showed no changes in the morphology of the given cells, and the expression of the neuroretinal genes such as OTX2, NRL, and PKCα. Moreover, recoverin was upregulated upon miR-183/-96/-182 overexpression in cultured hBMSCs. Ectopic overexpression of the miR-183 cluster could further increase the expression of CRX and RHO at the messenger RNA (mRNA) and protein levels. Furthermore, the data indicated that the miR-183 cluster could serve as a crucial function in photoreceptor cell differentiation. In fact, miRNAs could be assumed as potential targets to exploit silent neuronal differentiation. Ultimately, it was suggested that in vitro overexpression of miR-183 cluster could trigger reprogramming of the hBMSCs to retinal neuron fate, especially photoreceptor cells.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Overexpression of MiR-183/96/182 Triggers Retina-Like Fate in Human Bone Marrow-Derived Mesenchymal Stem Cells (hBMSCs) in Culture

Mahmoudian-Sani

Forouzanfar

Asgharzade

et al. 2019

Journal of Ophthalmology

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“…miRNAs exist as several distinct isoforms called isomiRs, which exhibit heterogeneous ends and lengths ( 1 , 2 ). Cellular isomiR profiles change dynamically during animal development and cell differentiation ( 3 , 4 ). Dysregulated isomiR profiles are linked with cancers ( 5–9 ).…”

Section: Introductionmentioning

confidence: 99%

Dicer partner protein tunes the length of miRNAs using base-mismatch in the pre-miRNA stem

Zhu

Kandasamy

Fukunaga

2018

Nucleic Acids Research

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Dicer partner proteins Drosophila Loquacious-PB (Loqs-PB) and human TRBP tune the length of miRNAs produced by Dicer from a subset of pre-miRNAs and thereby alter their target repertoire, by an unknown mechanism. Here, we developed a novel high-throughput method that we named Dram-seq (Dice randomized pre-miRNA pool and seq) to study length distributions of miRNAs produced from thousands of different pre-miRNA variants. Using Dram-seq, we found that a base-mismatch in the pre-miRNA stem can alter the length of miRNAs compared with a base-pair at the same position in both Drosophila and human, and is important for the miRNA length tuning by Loqs-PB. Loqs-PB directly bound base-mismatched nucleotides in the pre-miRNA stem. We speculate that Loqs-PB tunes miRNA length by changing the conformation of base-mismatched nucleotides in the pre-miRNA stem to that of base-paired ones and thereby altering the distance of the pre-miRNA stem.

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“…Mostly miRNAs are combined with the 3' end of the untranslated regions (3'UTR) of target mRNAs, resulting in their degradation or inhibition of translation (5). Importantly, miRNAs have acquired extensive attention recently as important regulators in a wide range of biological processes, such as immune response (6), angiogenesis (7), differentiation (8), cell proliferation and metastasis (9,10). Emerging studies have shown that dysreglated miRNAs function as either carcinogenic factors or tumor suppressors in a variety of cancers, such as miR-575 promoting growth and invasion of non-small cell lung cancer (11), miR-582-5p inhibiting proliferation of hepatocellular carcinoma by targeting CDK1 and AKT3 (12), miR-22 acting as an antitumor gatekeeper in de novo acute myeloid leukemia (AML) (13).…”

Section: Introductionmentioning

confidence: 99%

Low expression of microRNA-320b correlates with tumorigenesis and unfavorable prognosis in glioma

Liu

et al. 2017

Oncology Reports

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Accumulating evidence demonstrates that dysregulated microRNAs (miRNAs) play a critical role in tumorigenesis and progression of various cancers. miR-320b, a member of miR‑320 family, was revealed downregulated in numerous human cancers, including nasopharyngeal carcinoma and colorectal cancer. However, the function of miR‑320b in human glioma remained poorly defined. In this study, we report that miR‑320b was lowly expressed in glioma tissues and cell lines in contrast with controls, being closely correlated with histological malignancy of glioma. Furthermore, patients with low expression of miR‑320b were associated with poor prognostic outcomes. In vitro functional assays indicated that overexpression of miR‑320b could markedly enhance cell apoptosis rate and suppress cell proliferation, migration and invasion. miR-320b mimic impaired cell cycle and metastasis through inhibiting the expression of G1/S transition key regulator Cyclin D1 as well as decreasing the expression level of MMP2 and MMP9. Additionally, upregulation of miR‑320b could markedly promote apoptosis by increasing the level of Bax and reducing Bcl-2 expression in glioma. Taken together, our data suggested that miR‑320b might serve as a novel prognostic marker and potential therapeutic target for glioma.

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Variability of miRNA expression during the differentiation of human embryonic stem cells into retinal pigment epithelial cells

Cited by 13 publications

References 82 publications

Overexpression of MiR-183/96/182 Triggers Retina-Like Fate in Human Bone Marrow-Derived Mesenchymal Stem Cells (hBMSCs) in Culture

Overexpression of MiR-183/96/182 Triggers Retina-Like Fate in Human Bone Marrow-Derived Mesenchymal Stem Cells (hBMSCs) in Culture

Dicer partner protein tunes the length of miRNAs using base-mismatch in the pre-miRNA stem

Low expression of microRNA-320b correlates with tumorigenesis and unfavorable prognosis in glioma

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