Variability in the Content of the Constituents of<i>Hypericum perforatum</i>L. and Some Commercial Extracts

Bergonzi, Maria Camilla; Bilia, Anna Rita; Gallori, Sandra; Guerrini, D.; Vincieri, Franco Francesco

doi:10.1081/ddc-100105173

Cited by 63 publications

(42 citation statements)

References 6 publications

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“…Kukric et al (2013) evaluated the antioxidative effect of dried ethanolic extract of E. arvense, which had an IC50 and AAI (antioxidant activity index) of 13.5 and 3.9, respectively. Although present findings are somehow similar to results of those earlier studies, differences in extraction and activity methods, climate, soil, environmental factors, diseases, and pesticide treatments, harvest time, drying and storage methods and plant parts used in analyses may significantly affect the antioxidant activity of plants (Bergonzi et al, 2001;Wang and Zheng, 2001). In previous scientific studies, all over the world on nutritive composition of wild plants high quantities of minerals, especially K, Na, Ca, P, Mg (Guil Guerrero et al, 1998;Agrahar-Murugkar and Subbulakshmi, 2005) are indicated.…”

Section: Discussioncontrasting

confidence: 32%

In Vitro Screening of Natural Drug Potentials for Mass Production

Yaldız

Çalışkan

Aka

2017

Not Bot Horti Agrobo

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Methanolic extracts of 16 plant species, used in herbal and traditional medicinal preparations, collected from the same geographic region (Golcuk-Seben/Bolu, Turkey) were evaluated for their relative total phenolic contents, total antioxidant activities and their mineral contents in both dried plant samples and methanolic extracts. These species included Hypericum perforatum L., Thymus sipyleus Boiss., Equisetum arvense L., Achillea millefolium L., Tanacetum parthenium L., Thymus leucostomus Hausskn. & Velen., Usnea longissima, Herniaria incana Boiss., Teucrium polium L., Stachys bithynica Boiss., Anthemis nobilis L., Sambucus ebulus L., Berberis vulgaris L., Malus communis Desf., Matricaria chamomilla L. and Scorzonera laciniata L. Spectrophotometric Folin-Ciocalteu and DPPH free radical scavenging activity methods were performed to detect the total phenolic contents and total antioxidant activities of the methanolic extracts, respectively. The results show that the fruits of Berberis vulgaris L., have the highest antioxidant activity and aerial parts of Tanacetum parthenium L. have the highest total phenolics (92.62%). Mineral contents were determined by inductively coupled -mass spectrometry (ICP-MS). All dried plant samples and methanolic extracts contained high amounts of Si, Cu, Fe and Ca. The highest levels of Si, Cu, Fe and Ca were found in Equisetum arvense L., Anthemis nobilis L., and Thymus sipyleus Boiss. dried plant samples, whereas Se was only detected in Usnea longissima. The highest levels of Si, Cu, Fe and Ca were found in the methanolic extracts of Usnea longissima, Anthemis nobilis L. and Thymus sipyleus Boiss. respectively.

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Section: Discussioncontrasting

confidence: 32%

In Vitro Screening of Natural Drug Potentials for Mass Production

Yaldız

Çalışkan

Aka

2017

Not Bot Horti Agrobo

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“…Recently, H. perforatum naphthodianthrones have also been studied for their possible application in the photodynamic therapy of cancer [8]. The concentration of these constituents in the plant is particularly low, as mentioned above, varying from 0.01 to 0.03% and even in alcoholic or hydroalcoholic extracts it may reach up to 0.1 -0.4% [9]. Investigations in order to find other Hypericum sp.…”

Section: Introductionmentioning

confidence: 99%

Rapid and efficient purification of naphthodianthrones from St. John's wort extract by using liquid–liquid extraction and SEC

Karioti¹,

Vincieri

Bilia

2009

J of Separation Science

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Rapid and efficient purification of naphthodianthrones from St. John's wort extract by using liquid-liquid extraction and SEC Hypericin and pseudohypericin, the main naphthodianthrones present in Hypericum species are among the most promising natural products, but the research concerning their biological activities is hindered by their low content in the plant. In this paper a method for the rapid isolation of hypericin and pseudohypericin from Hypericum perforatum hydro-alcoholic dried extracts has been developed. Briefly, the method consists of a partition of the extract between organic and aqueous layers and further purification of the richest extract in naphthodianthrones with Sephadex LH-20 column chromatography. A final separation of hypericin from pseudohypericin was achieved using Sephadex LH-60 column chromatography. All partitions were carried out in triplicate and monitored by LC-MS and NMR analyses. The best results were obtained by successive extraction with n-hexane, Et 2 O and EtOAc. A three-step fractionation resulted in 98% content in total naphthodianthrones. To the best of our knowledge this is the first report on the separation of hypericin from pseudohypericin using size exclusion chromatography.

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“…The HPLC system consisted of a HP 1100 L instrument with a diode array detector managed by a HP 9000 (Bergonzi et al, 2001). Before the HPLC analysis, each sample was filtered through a cartridge-type sample filtration unit with a polytetrafluoroethylene (PTFE) membrane (d = 13 mm, porosity 0.45 μm, Lida manufacturing Corp.) and immediately injected.…”

Section: Hplc-dad and Hplc-ms Drug Analysesmentioning

confidence: 99%

“…To evaluate the constituents' content, an HPLC analysis was performed using a method described in the literature (Bergonzi et al, 2001). Identification of the constituents was performed using combined HPLC-diode array detection (DAD) analysis and HPLC-thermospray mass spectrometry.…”

Section: Hplc-dad and Hplc-ms Drug Analysesmentioning

confidence: 99%

Pharmacological in vivo test to evaluate the bioavailability of some St. John's wort innovative oral preparations

Bilia¹,

Bergonzi²,

Isacchi³

et al. 2006

Planta Med

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In this study, the optimisation of biopharmaceutical properties of a dried commercial extract of St John's Wort were evaluated employing the in vivo forced swimming test (FST). Three new dosage forms containing β β β β β-cyclodextrin and surfactants (SDS, ASC8) were compared in the FST with the commercial extract. The commercial extract showed antidepressant activity in mice after 60 min at a dosage of 100 mg/kg. The same antidepressant activity appeared in 30 min with a micellar solution of SDS containing the same quantity of extract (100 mg/kg), while with micelles of ASC8 the effect appeared at 15 min and with a dosage of 30 mg/ kg. In the case of β β β β β-cyclodextrin the best results were obtained at 30 min, administering 60 mg/kg of the extract. Finally, the influence of the formulations on the water solubility of the constituents of the extract is reported. The tensides dramatically enhanced solubility, in particular that of the more lipophilic compounds, in the case of β β β β β-cyclodextrin this effect was very pronounced for flavonoids and biapigenin, lower for hypericins and practically insignificant for hyperforins.

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Variability in the Content of the Constituents ofHypericum perforatumL. and Some Commercial Extracts

Cited by 63 publications

References 6 publications

In Vitro Screening of Natural Drug Potentials for Mass Production

In Vitro Screening of Natural Drug Potentials for Mass Production

Rapid and efficient purification of naphthodianthrones from St. John's wort extract by using liquid–liquid extraction and SEC

Pharmacological in vivo test to evaluate the bioavailability of some St. John's wort innovative oral preparations

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