Vanillin confers antifungal drug synergism in Candida albicans by impeding CaCdr2p driven efflux

Saibabu, Venkata; Fatima, Zeeshan; Singh, Shweta; Khan, Luqman Ahmad; Hameed, Saif

doi:10.1016/j.mycmed.2019.100921

Cited by 10 publications

(6 citation statements)

References 34 publications

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“…In the vanillin–cinnamic acid combination, the FIC indices for the planktonic growth inhibition were ≤0.31 ( p < 0.05), indicating that the combination was synergistic in action in all the spoilage yeasts (FICI ≤ 0.5). It has been reported that vanillin could interact synergistically with membrane targeting antifungals, leading to inhibition of fungal drug efflux pumps [ 35 ]. In another study, vanillin led to the disruption of fungal cell surface integrity, dysfunctional mitochondria, and DNA damage when used as a single agent [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

Activity of Binary Combinations of Natural Phenolics and Synthetic Food Preservatives against Food Spoilage Yeasts

Kimani

Takó

Veres

et al. 2023

Foods

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Natural compounds are a suitable alternative to synthetic food preservatives due to their natural origin and health-promoting properties. In the current study, phenolic–phenolic and phenolic–synthetic combinations were tested for their antibiofilm formation, anti-planktonic growth, and anti-adhesion properties against Debaryomyces hansenii, Wickerhamomyces anomalus (formerly Pichia anomala), Schizosaccharomyces pombe, and Saccharomyces cerevisiae. The phenolics were vanillin and cinnamic acid, while the synthetic preservatives were sodium benzoate, potassium sorbate, and sodium diacetate. The vanillin–cinnamic acid combination had synergistic effect in all the tested yeasts for the biofilm inhibition with a fractional inhibitory concentration index (FICI) of ≤0.19 for W. anomalus, 0.25 for S. pombe, 0.31 for S. cerevisiae, and 0.5 for D. hansenii. Most of the phenolic–synthetic combinations had indifferent interaction regarding biofilm formation. The vanillin–cinnamic acid combination also had higher activity against spoilage yeasts adhesion on the abiotic surface and planktonic growth compared to the phenolic–synthetic combinations. For the phenolic–synthetic anti-planktonic activity, synergistic interaction was present in all the vanillin–synthetic combinations in S. pombe, vanillin–sodium benzoate and vanillin–potassium sorbate in S. cerevisiae, vanillin–sodium benzoate in W. anomalus, and cinnamic acid–sodium diacetate in S. pombe. These results suggest a novel antimicrobial strategy that may broaden the antimicrobial spectrum and reduce compound toxicity against food spoilage yeasts.

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Section: Discussionmentioning

confidence: 99%

Activity of Binary Combinations of Natural Phenolics and Synthetic Food Preservatives against Food Spoilage Yeasts

Kimani

Takó

Veres

et al. 2023

Foods

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“…We used Rho123 as a membrane-potential-sensitive cationic fluorophore [ 45 ] to show that it was not able to leave the mitochondrion due to decreased membrane potential as a result of the 5h -treated cell death ( Figure 2 , Figure 3 and Figure 4 , Figure 9 and Figure 10 as well as Tables S4 and S5 ). We concluded that 5h can be mitochondrial inhibitor of C. albicans ref and C. neoformans .…”

Section: Discussionmentioning

confidence: 99%

“…Concentration of Rho123 was calculated using formula: C = (E − Blank − 1151.2) × 10/556.91; where: E—emission; Blank—emission of PBS/glucose medium; 1151.2 and 556,91—coefficients of rhodamine standard curve; 10—dilution coefficient. Decrease of the Rho123 content was determined using the formula: ΔC% = [C(Test) − C(Control)]/C(Control) × 100; where: C(Test)—concentration of rhodamine in tested samples; C(Control)—concentration of rhodamine in control samples [ 45 ].…”

Section: Methodsmentioning

confidence: 99%

“…Examination was preformed using DCFDA/H2DCFDA kit (Thermo Fisher Scientific, Waltham, MA, USA) [ 45 ]. C. albicans SC5314, C. albicans SPZ 176 clinical isolate and C. neoformans SPZ 173 clinical isolate were prepared as previously described.…”

Section: Methodsmentioning

confidence: 99%

“…Fluorescence was measured with extinction at 485 nm and emission at 530 nm using Synergy H4 Hybrid Reader (BioTek Instruments, Winooski, VT, USA). Change in ROS concentration was calculated using formula: ΔC = [E(Test) − E(Control)] × 100%/E(Control); where ΔC—change in ROS concentration; E(Test)—fluorescence of test samples; E(Control)—fluorescence of negative control [ 45 ].…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

The Antifungal Action Mode of N-Phenacyldibromobenzimidazoles

et al. 2021

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Our study aimed to characterise the action mode of N-phenacyldibromobenzimidazoles against C. albicans and C. neoformans. Firstly, we selected the non-cytotoxic most active benzimidazoles based on the structure–activity relationships showing that the group of 5,6-dibromobenzimidazole derivatives are less active against C. albicans vs. 4,6-dibromobenzimidazole analogues (5e–f and 5h). The substitution of chlorine atoms to the benzene ring of the N-phenacyl substituent extended the anti-C. albicans action (5e with 2,4-Cl2 or 5f with 3,4-Cl2). The excellent results for N-phenacyldibromobenzimidazole 5h against the C. albicans reference and clinical isolate showed IC50 = 8 µg/mL and %I = 100 ± 3, respectively. Compound 5h was fungicidal against the C. neoformans isolate. Compound 5h at 160–4 µg/mL caused irreversible damage of the fungal cell membrane and accidental cell death (ACD). We reported on chitinolytic activity of 5h, in accordance with the patterns observed for the following substrates: 4-nitrophenyl-N-acetyl-β-d-glucosaminide and 4-nitrophenyl-β-d-N,N′,N″-triacetylchitothiose. Derivative 5h at 16 µg/mL: (1) it affected cell wall by inducing β-d-glucanase, (2) it caused morphological distortions and (3) osmotic instability in the C. albicans biofilm-treated. Compound 5h exerted Candida-dependent inhibition of virulence factors.

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Plant-derived nanotherapeutic systems to counter the overgrowing threat of resistant microbes and biofilms

Asghar

Khan

Salman

et al. 2021

Advanced Drug Delivery Reviews

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Vanillin confers antifungal drug synergism in Candida albicans by impeding CaCdr2p driven efflux

Cited by 10 publications

References 34 publications

Activity of Binary Combinations of Natural Phenolics and Synthetic Food Preservatives against Food Spoilage Yeasts

Activity of Binary Combinations of Natural Phenolics and Synthetic Food Preservatives against Food Spoilage Yeasts

The Antifungal Action Mode of N-Phenacyldibromobenzimidazoles

Plant-derived nanotherapeutic systems to counter the overgrowing threat of resistant microbes and biofilms

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