1982
DOI: 10.1021/jf00114a020
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Vanillin assay for proanthocyanidins (condensed tannins): modification of the solvent for estimation of the degree of polymerization

Abstract: Purified tannin from sorghum grain (NK 300) was provided by Dr. Haslam. Tannin from a high-tannin sorghum grain (BR 54) was purified by our standard technique (Hagerman and Butler, 1980) by A. Hagerman. Samples to be used for determining extinction coefficients were dried overnight over P205 at room temperature.Assays. Vanillin assays in methanol were carried out as recommended by Price et al. (1978), in a 30 °C water bath with a reaction time of 20 min. The vanillin reagent contained 4% concentrated HC1 and… Show more

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Cited by 211 publications
(130 citation statements)
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“…The content of sinapine was determined according to a standard method used in rapeseed meal (Cai and Arntfield, 2001;Li and El Rassi, 2002). The content of tannins was measured by the method of Butler et al (1982). Content of total glucosinolates was determined by high pressure liquid chromatography using sinigrin as an internal standard (ISO, 1994).…”
Section: Discussionmentioning
confidence: 99%
“…The content of sinapine was determined according to a standard method used in rapeseed meal (Cai and Arntfield, 2001;Li and El Rassi, 2002). The content of tannins was measured by the method of Butler et al (1982). Content of total glucosinolates was determined by high pressure liquid chromatography using sinigrin as an internal standard (ISO, 1994).…”
Section: Discussionmentioning
confidence: 99%
“…Total alkaloids were analyzed according to the method described by Skolik and Wiewiorowski (1959). Phytates were determined as phytic acid using HPLC method (Newkirk and Classen 1998); condensed tannins as described by Buttler et al (1982). NSP were analyzed using GLC method as described by Englyst (1989).…”
Section: Analyses and Statisticsmentioning
confidence: 99%
“…There are many publications available on tannin analyses (Folin & Ciocalteu, 1927;Hagerman & Butler, 1978;Butler et al, 1982;Makkar, 1989;Souquet et al, 1996;Giner-Chavez et al, 1997;Price & Butler, 1997;Sun et al, 1998;Schofield et al, 2001;Hagerman, 2002;Harbertson et al, 2003;De Beer et al, 2004;Moris & Silber, 2006;Sarneckis et al, 2006;Seddon & Downey, 2008). However, not all of these analyses are applicable to grape tannins.…”
Section: Determination Of Total Polyphenols and Tanninsmentioning
confidence: 99%