Valuation of three different anaesthetic protocols on complete blood count and biochemical parameters on Wistar rats

Abstract

ABSTRACT.The objective of the current study was to determine the impact of three different anaesthetic protocols on blood and biochemical parameters. Eighteen female Wistar rats (HsdOla:WI) 3 months old, weighting 197.09 ± 7.39 g were used. Baseline blood collection was performed in all animals from the lateral coccygeal vein for evaluation of glucose. The animals were then randomly allocated to receive one of three different anaesthetic protocols: dexmedetomidine/ketamine intramuscularly (0.25 mg/kg and 50 mg/kg respectively), or isoflurane 0.2 ml on cotton inside a syringe case, or isoflurane administered by vaporiser (5% induction and 2-3% maintenance of anaesthesia, delivered in oxygen flow 1 L/min). Blood samples were collected from caudal vena cava for complete blood count and biochemical analysis, while the lung and the liver were harvested for histological evaluation. Comparison between groups, as far as complete blood count parameters is concerned, revealed statistical significant differences in red blood cells, haematocrit and haemoglobin values with lower values being observed in vaporiser anaesthesia group. Furthermore, within group comparison revealed statistical significant differences for glucose in all three groups. No evidence of inflammatory, degenerative neoplastic or toxicity related lesions were observed during histological evaluation of the lung and liver.Keywords: Anaesthesia; Rats; Blood parameters ΠΕΡΙΛΗΨΗ. Ο στόχος της παρούσας μελέτης ήταν να προσδιοριστεί η επίδραση τριών διαφορετικών πρωτοκόλλων αναισθησίας στις αιματολογικές και βιοχημικές παραμέτρους. Για το σκοπό αυτό χρησιμοποιήθηκαν δεκαοχτώ θηλυκοί επίμυες Wistar (WI HsdOla) ηλικίας 3 μηνών, που ζύγιζαν 197,09 ± 7,39 g. Η αρχική αιμοληψία πραγματοποιήθηκε σε όλα τα ζώα από την πλάγια κοκκυγική φλέβα για την αξιολόγηση των επιπέδων γλυκόζης. Τα ζώα στη συνέχεια χωρίστηκαν τυχαία σε 3 ομάδες ώστε να λάβουν ένα από τα τρία διαφορετικά αναισθητικά πρωτόκολλα: δεξμεδετομιδίνη/κεταμίνη ενδομυϊκά (0,25 mg/kg και 50 mg/kg αντίστοιχα), βαμβάκι εμποτισμένο με 0,2 ml ισοφλουρανίου τοποθετημένο μέσα σε σύριγγα, ή ισοφλουράνιο χορηγούμενο με αναισθητική μηχανή (5% για εισαγωγή στην αναισθησία και 2-3% για συντήρηση , ροή οξυγόνου 1 L/min). Συλλέχθηκαν δείγματα αίματος από την οπίσθια κοίλη φλέβα για τη γενική αίματος και τις βιοχημικές αναλύσεις, ενώ συλλέχθηκαν ο πνεύμονας και το ήπαρ για ιστολογική αξιολόγηση. Η σύγκριση μεταξύ των ομάδων, όσον αφορά τις αιματολογικές παραμέτρους κατέδειξε στατιστικά σημαντικές διαφορές στις τιμές των ερυθρών αιμοσφαιρίων, του αιματοκρίτη και της αιμοσφαιρίνης με τις χαμηλότερες τιμές να παρατηρούνται στην ομάδα με τη χρήση αναισθητικής μηχανής. Η σύγκριση εντός της ομάδας κατέδειξε στατιστικά σημαντικές διαφορές στα επίπεδα γλυκόζης και για τις τρεις ομάδες. Δεν παρατηρήθηκαν ενδεί-ξεις αλλοιώσεων σχετικών με φλεγμονή, εκφυλιστική νεοπλασία ή τοξικότητα κατά την ιστολογική αξιολόγηση του πνεύμονα και του ήπατος.

Assessment of the haematological profile after appendectomy using linear titanium stapler clips: An experimental study in rats

“…The results were found to be significant at p < 0.05 in all the animals in the second experimental endoloop vicryl ligatures group, as well as in the titanium stapler clips sub-group on day 28. The values obtained are at the lower limits of the physiological range, or lower in some sub-groups, which clearly indicates the beginning of hypochromia, in line with similar research by Lelovas et al (2017), 22 and Bajrić et al (2020). 3 The MCHC and MCV count parameters varied within the physiological range, but they were also significantly different to the control in all three experimental vicryl groups.…”

Minimum Information in In Vivo Research

“…The authors demonstrated differential effects of the different anaesthetics with regard to some of the measured parameters and differences across the sex and species used, thus demonstrating the importance of understanding the impact that an anaesthetic can haveeven on ex vivo readoutsand to include appropriate controls. A similar study was conducted by Lelovas et al which further highlights the importance of concise and accurate reporting of the use of anaesthetics in the collection of biological samples for biochemical readouts since their use can have a significant impact on outcome (Lelovas et al 2017). Watters and Goodman published a comparison of basic methods in clinical studies and in in vitro tissue and cell culture studies reported in three anaesthesia journals (Watters and Goodman 1999).…”

Good Research Practice in Non-Clinical Pharmacology and Biomedicine

“…The authors demonstrated differential effects of the different anaesthetics with regard to some of the measured parameters and differences across the sex and species used, thus demonstrating the importance of understanding the impact that an anaesthetic can haveeven on ex vivo readoutsand to include appropriate controls. A similar study was conducted by Lelovas et al which further highlights the importance of concise and accurate reporting of the use of anaesthetics in the collection of biological samples for biochemical readouts since their use can have a significant impact on outcome (Lelovas et al 2017). Watters and Goodman published a comparison of basic methods in clinical studies and in in vitro tissue and cell culture studies reported in three anaesthesia journals (Watters and Goodman 1999).…”