2018
DOI: 10.1371/journal.pntd.0006922
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Abstract: BackgroundThe World Health Organization has targeted elimination of visceral leishmaniasis (VL) in the Indian subcontinent (ISC) by 2020. Despite distinctive decline seen in the number of VL cases in ISC, there is still a quest for development of a diagnostic test which has the utility for detection of active infection and relapse cases and as a test of cure. The present study validated the sensitivity and specificity of SYBR Green I based closed tube LAMP assay reported by us for diagnosis of VL.MethodologyTh… Show more

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Cited by 37 publications
(38 citation statements)
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“…In addition, sensitivity and specificity of serological tests are very variable in the literature, also considering the choice of a gold standard. In fact, some authors considered the combination of at least two techniques [6,36], as in our study, while some other authors used a Bayesian method to evaluate the performances of the tests [10,40] In the last years, many molecular techniques have been developed for the diagnosis of leishmaniosis [25]. In particular, the Rt-PCR resulted very useful to evaluate follow-up, nevertheless, this molecular technique is not widely used due to its complexity, and it requires expensive laboratory equipment and expert staff [25,41].…”
Section: Discussionmentioning
confidence: 99%
See 2 more Smart Citations
“…In addition, sensitivity and specificity of serological tests are very variable in the literature, also considering the choice of a gold standard. In fact, some authors considered the combination of at least two techniques [6,36], as in our study, while some other authors used a Bayesian method to evaluate the performances of the tests [10,40] In the last years, many molecular techniques have been developed for the diagnosis of leishmaniosis [25]. In particular, the Rt-PCR resulted very useful to evaluate follow-up, nevertheless, this molecular technique is not widely used due to its complexity, and it requires expensive laboratory equipment and expert staff [25,41].…”
Section: Discussionmentioning
confidence: 99%
“…In the last years, many molecular techniques have been developed for the diagnosis of leishmaniosis [ 25 ]. In particular, the Rt-PCR resulted very useful to evaluate follow-up, nevertheless, this molecular technique is not widely used due to its complexity, and it requires expensive laboratory equipment and expert staff [ 25 , 41 ].…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…However, this cannot be avoided for DNA intercalating dyes, such as SYBR green I, as they must be added to the LAMP reaction following amplification. To overcome this limitation, closed‐tube LAMP has been developed recently (Dixit et al ). Here, we obtained the same favourable results by adding an extra protectant in the tube to prevent the reaction mixture from contacting with air directly, and fully inactivating the Bst enzyme before adding DNA intercalating dye.…”
Section: Discussionmentioning
confidence: 99%
“…The sensitivity of these tests is in general modest and varies enormously. Nucleic acid amplification tests have better sensitivity [3] but are relatively more complicated, expensive, and are restricted to referral hospitals and advanced research centers [4][5][6][7][8][9][10][11][12]. Due to the limitations of these invasive approaches, the presence of anti-parasite antibodies in serum is also routinely used to diagnose VL [13,14].…”
Section: Introductionmentioning
confidence: 99%