Validation of simple and robust protocols for high‐resolution lung proton MRI in mice

Żurek, Magdalena; Bessaad, Amine; Cieslar, K.; Crémillieux, Yannick

doi:10.1002/mrm.22360

Cited by 47 publications

(51 citation statements)

References 28 publications

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“…delivery of contrast agents was made possible with the use of appropriate UTE MRI sequences using radial k-space sampling, less sensitive to cardiorespiratory motion than Cartesian MR acquisitions (14)(15)(16)(17). As a consequence, freebreathing acquisitions can be performed with limited impact on lung images signal-to-noise ratio, sharpness, and spatial resolution (16).…”

Section: Discussionmentioning

confidence: 99%

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Targeting and in vivo imaging of non-small–cell lung cancer using nebulized multimodal contrast agents

Bianchi

Dufort

Lux

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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One of the main reasons for the dismal prognosis of lung cancer is related to the late diagnosis of this pathology. In this work, we evaluated the potential of optimized lung MRI techniques and nebulized ultrasmall multimodal gadolinium-based contrast agents [ultrasmall rigid platforms (USRPs)] as a completely noninvasive approach for non-small-cell lung cancer (NSCLC) in vivo detection. A mouse model of NSCLC expressing the luciferase gene was developed. Ultrashort echo-time free-breathing MRI acquisitions were performed before and after i.v. or intrapulmonary administration of the nanoparticles to identify and segment the tumor. After orotracheal or i.v. administration of USRPs, an excellent colocalization of the position the tumor with MRI, bioluminescence and fluorescence reflectance imaging, and histology was observed in all mice. Significantly higher signal enhancements and contrast-to-noise ratios were observed with orotracheal administration using lower doses, reducing the toxicity issues and the interobserver variability in tumor detection. The observations suggested the existence of an unknown original mechanism (different from the enhanced permeability and retention effect) responsible for this phenomenon. MRI and USRPs were shown to be powerful imaging tools able to detect, quantify, and longitudinally monitor the development of submillimetric NSCLCs. The absence of ionizing radiation and high resolution MRI, along with the complete noninvasiveness and good reproducibility of the proposed protocol, make this technique potentially translatable to humans. To our knowledge this is the first time that the advantages of an orotracheal administration route are demonstrated for the investigation of the pathomorphological changes due to NSCLCs. lung tumor | magnetic resonance imaging | bioluminescence imaging | gadolinium-based nanoparticles

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Section: Discussionmentioning

confidence: 99%

“…Briefly, these sequences are based on ultrashort echo time (UTE) and radial sampling of the k-space (10, 13). UTE proton MRI sequences have been demonstrated to be extremely efficient for limiting motion and susceptibility artifacts and for enhancing MRI signal intensity of the lung tissue (14)(15)(16)(17)(18)(19).…”

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confidence: 99%

Targeting and in vivo imaging of non-small–cell lung cancer using nebulized multimodal contrast agents

Bianchi

Dufort

Lux

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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“…As compared to a scan-synchronous ventilation approach, used for a motion artifacts reduction, this approach is less invasive, enables higher throughput and it is well adapted for longitudinal investigations. In future studies, this technique can be used in conjunction with a retrospective self-gating approach (14) to investigate emphysema independent on the breathing phase.…”

Section: Discussionmentioning

confidence: 99%

Longitudinal and noninvasive assessment of emphysema evolution in a murine model using proton MRI

Żurek

Boyer

Caramelle

et al. 2011

Magnetic Resonance in Med

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Ultrashort echo time (550 ms) MR imaging was implemented to track the emphysema development in mice lung challenged with elastase. Two parameters, namely, signal intensity and T 2 *, were used to monitor the disease evolution. Nine mice were imaged before and at 24 h as well as at 3 and 8 weeks after elastase instillation. Five mice instilled with saline served as controls. At week 8, the mean normalized signal intensity 6 SD was 0.89 6 0.20 for healthy controls and 0.64 6 0.10 for animals with emphysema. Similarly, a reduced value of T 2 * (1.27 6 0.35 ms vs 0.96 6 0.18 ms) was found in the emphysema group. The mean signal intensity drop and the reduction of T 2 * were prominent at 3 weeks following elastase instillation and stabilized between 3 and 8 weeks. The results indicated an excellent agreement between MR findings and histological morphometry (signal intensity, r 5 20.78, P 5 0.004; T 2 *, r 5 20.78, P 5 0.001). This result shows that proton MRI allows structural changes at alveolar level to be monitored longitudinally. This technique, applied routinely in preclinical trials will represent a valuable tool for assessment of drug therapy efficacy. Magn Reson Med 68:898-904,

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“…Since UTE-MRI of the lung was first demonstrated in the early 1990s on a 1.5 T system (28), most of the subsequent studies have been performed on animal MR systems at higher field strengths (14,15,18,22). Only recently, investigators performed UTE-MRI of the lung in animal models using clinical systems at 3.0 T (16,17).…”

Section: Te=01 Ms Te=21 Msmentioning

confidence: 99%

“…Therefore, previous attempts at measuring lung water by MRI potentially underestimated the true water content by up to 40% (10). Half radiofrequency excitations and subsequent radial mapping from the center of the k-space have enabled TEs of approximately 0.1 ms, which are sufficiently short to detect the peak amplitude of the signal that determines the true proton density (12)(13)(14)(15)(16)(17)(18).…”

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confidence: 99%

Ultrashort echo time MRI of pulmonary water content: assessment in a sponge phantom at 1.5 and 3.0 Tesla

Molinari¹,

Madhuranthakam²,

Lenkinski³

et al. 2013

Diagn Interv Radiol

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ORIGINAL ARTICLE PURPOSE We aimed to develop a predictive model for lung water content using ultrashort echo time (UTE) magnetic resonance imaging (MRI) and a sponge phantom. MATERIALS AND METHODSImage quality was preliminarily optimized, and the signalto-noise ratio (SNR) of UTE was compared with that obtained from a three-dimensional fast gradient echo (FGRE) sequence. Four predetermined volumes of water (3.5, 3.0, 2.5, and 2.0 mL) were soaked in cellulose foam sponges 1.8 cm 3 in size and were imaged with UTE-MRI at 1.5 and 3.0 Tesla (T). A multiple echo time experiment (range, 0.1-9.6 ms) was conducted, and the T2 signal decay curve was determined at each volume of water. A three-parameter equation was fitted to the measured signal, allowing for the calculation of proton density and T2*. The calculation error of proton density was determined as a function of echo time. The constants that allowed for the determination of unknown volumes of water from the measured proton density were calculated using linear regression. RESULTS UTE-MRI provided excellent image quality for the four phantoms and showed a higher SNR, compared to that of FGRE. Proton density decreased proportionally with the decreases in both lung water and field strength (from 3.5 to 2.0 mL; proton density range at 1.5 T, 30.5-17.3; at 3.0 T, 84.2-41.5). Minimum echo time less than 0.6 ms at 1.5 T and 1 ms at 3.0 T maintained calculation errors for proton density within the range of 0%-10%. The slopes of the lines for determining the unknown volumes of water with UTE-MRI were 0.12±0.003 at 1.5 T and 0.05±0.002 at 3.0 T (P < 0.0001). CONCLUSIONIn a sponge phantom imaged at 1.5 and 3.0 T, unknown volumes of water can be predicted with high accuracy using UTE-MRI.

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Validation of simple and robust protocols for high‐resolution lung proton MRI in mice

Cited by 47 publications

References 28 publications

Targeting and in vivo imaging of non-small–cell lung cancer using nebulized multimodal contrast agents

Targeting and in vivo imaging of non-small–cell lung cancer using nebulized multimodal contrast agents

Longitudinal and noninvasive assessment of emphysema evolution in a murine model using proton MRI

Ultrashort echo time MRI of pulmonary water content: assessment in a sponge phantom at 1.5 and 3.0 Tesla

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