Validation of Meat Inspection Results for Taenia saginata Cysticercosis by PCR–Restriction Fragment Length Polymorphism

Geysen, Dirk; Kanobana, Kirezi; Victor, Björn; Rodríguez‐Hidalgo, Richar; Borchgrave, J. de; Brandt, Jef; Dorny, Pierre

doi:10.4315/0362-028x-70.1.236

Cited by 59 publications

(44 citation statements)

References 10 publications

(10 reference statements)

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“…Consequently, it can be argued that such programmes rather than the parasitic infection itself 375 380 385 390 395 which are causing the economic losses. Furthermore, current routine meat inspection has a very low sensitivity (Geysen et al, 2007). Therefore most infected beef enters the human food chain regardless.…”

Section: Taenia Saginatamentioning

confidence: 99%

One world health: Socioeconomic burden and parasitic disease control priorities

Torgerson¹

2013

Veterinary Parasitology

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Parasitic diseases present a considerable socio-economic impact to society. Zoonotic parasites can result in a considerable burden of disease in humans and substantive economic losses to livestock populations. Ameliorating the effects of these diseases may consist of attempts at eradicating specific diseases at a global level, eliminating them at a national or local level or controlling the disease to minimise incidence. Alternatively with some parasitic zoonoses it may only be possible to treat human and animal cases as they arise. The choice of approach will be determined by the potential effectiveness of a disease control programme, its cost and the cost effectiveness or cost benefit of undertaking the intervention. Furthermore human disease burden is being increasingly measured by egalitarian nonfinancial measures which do not sit comfortably with measurements of economic costs in livestock.This adds additional challenges to assessing socio-economic burdens of zoonotic diseases. Using examples from the group of neglected zoonotic diseases, information regarding the socio-economic effects of these diseases are reviewed and the use of this information in decision making with regard to control and treatment of these diseases is discussed.

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Section: Taenia Saginatamentioning

confidence: 99%

One world health: Socioeconomic burden and parasitic disease control priorities

Torgerson¹

2013

Veterinary Parasitology

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“…Moreover, this depends on the expertise of the meat inspector, indicating lack of sensitivity and objectivity. The cysticerci can be confounded with sarcocystis (Chiesa et al 2010), milk spots (de Aluja 1994), T. hydatigena cysticerci (Kedra et al 2001), hydatid cysts (Deplazes et al 2005) and unstructured piece of fat and left over of muscle fasciae (Geysen et al 2007). …”

Section: Introductionmentioning

confidence: 99%

PCR test for detecting Taenia solium cysticercosis in pig carcasses

Sreedevi

Hafeez

Kumar

et al. 2011

Trop Anim Health Prod

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Polymerase chain reaction (PCR) test was employed to detect Taenia solium DNA in muscle lesions for validation of the meat inspection results of slaughtered pigs. Two sets of oligonucleotide primers, one targeted against the large subunit rRNA gene (TBR primers) and the other targeted against cytochrome c oxidase subunit 1 gene (Cox1 primers) of T. solium were used in this study. On reactivity in PCR test, the TBR primers and the Cox1 primers yielded products of 286 and 984 bp, respectively, in cysticercosis positive cases. Both the sets of primers were found to be highly specific, since they did not yield any PCR product in negative controls. A total of 225 pig carcasses were screened for cysticercosis by meat inspection, out of which 25 carcasses with visible cysts (16 viable and 9 degenerated cysts) were also confirmed to be positive for cysticercosis in PCR test. However, out of the 35 carcasses with suspected lesions on meat inspection, only two were found to be positive for cysticercosis in PCR test. The detection limits for both the primer sets were analyzed. The TBR primer set could detect up to 10 pg of cysticercus DNA, whereas the Cox1 primer set could detect only up to 1 ng. It is evident from the study that PCR test is an efficient tool for validation of meat inspection results and also to rule out ambiguity in carcass judgment of suspected cases of porcine cysticercosis.

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“…Nevertheless, it is important to keep in mind that, even if meat inspection is highly specific (100%), its sensitivity is very poor (Dorny et al 2004, Geysen et al 2007.…”

Section: Methodology To Apply To Answer the Risk Questionmentioning

confidence: 99%

Specification of data collection on animal diseases to increase the preparedness of the AHAW panel to answer future mandates – CFP/EFSA/AHAW/2010/01

Bellet

Humblet

Swanenburg

et al. 2012

EFSA Supporting Publications

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Risk assessment is EFSA's core activity. The lack of quantitative data and standardized methodology to collect data for risk assessment may limit the use of formal and structured quantitative risk assessments and delay the production of accurate scientific opinions. The aim of this project was to develop a methodological framework to identify data needs in accordance with the risk questions and the relevant risk assessment methods. A review of AHAW panel opinions in the area of animal health, adopted between 2004 and 2010 (WP1) was conducted in order to identify and to categorize the most recurrent risk questions, the suitable risk assessment methods and data needs. Subsequently an inventory of the possible sources of required data and an assessment of their availability and accessibility were performed (WP2). Facts and metadata were distinguished from WP1 initial list of data needs and a metadata model for facts collection and documentation was then proposed (WP3). The outcomes and conclusions from the three first work packages was used to suggest a structured approach to collect data (facts and metadata) in regard to expected future risk questions. Based on four case studies related to Echinococcus multilocularis, E. granulosus, Porcine Reproductive and Respiratory Syndrome and Venezuelan Equine Encephalitis (WP4), we concluded that this approach could lead to more efficient preparatory data collection, and therefore enable more rapid response to new risk managers questions. KEY WORDSRisk assessment, risk questions, animal health, data, fact, metadata 1 EFSA-Q-2010-00903 2 Acknowledgement: This study was carried out within the framework of the EFSA art.36 project (CFP/EFSA/AHAW/2010/01). We warmly thank the ANSES, CVI and ULg consortium team. We are grateful to all the experts from Belgium, France and Netherlands, who helped evaluate the needed data related to WP4 case studies

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Validation of Meat Inspection Results for Taenia saginata Cysticercosis by PCR–Restriction Fragment Length Polymorphism

Cited by 59 publications

References 10 publications

One world health: Socioeconomic burden and parasitic disease control priorities

One world health: Socioeconomic burden and parasitic disease control priorities

PCR test for detecting Taenia solium cysticercosis in pig carcasses

Specification of data collection on animal diseases to increase the preparedness of the AHAW panel to answer future mandates – CFP/EFSA/AHAW/2010/01

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