Validation of an ultra high pressure liquid chromatographic method for the determination of biologically active amines in food

Simultaneous Determination of Biogenic Amines and Methylxanthines in Foodstuff—Sample Preparation with HPLC-DAD-FL Analysis

“…For methylxanthines, diode array detector (DAD) (Baranowska and Zydroń 2002;Baranowska et al 2006;Hicks et al 1996;Matissek 1997;Zydroń et al 2004) is widely used. Interest in application of ultra high-performance liquid chromatography has increased in the last few years (Tzanavarasa et al 2010;Dadáková et al 2009;Mayer et al 2010;Latorre-Moratalla et al 2009). Such method allows one to significantly shorten time of chromatographic analysis.…”

Biologically active amines in fermented and non-fermented commercial soybean products from the Spanish market

“…Before chromatographic analysis, perchloric extracts were passed through a 0.22 lm filter (GHP, Waters Corp, Milford, MA). Twelve biogenic amines and polyamines, namely octopamine, dopamine, TY, PU, CA, HI, agmatine (AG), serotonin, PHE, SPD, TR and SPM, were determined following the method described by Latorre-Moratalla et al (2009). Amine separation was performed through a Acquity UPLC BEH C-18 reverse phase column (Waters corp., Milford, MA, USA), followed by a post-column derivatization with o-phthalaldehyde and spectrofluorimetric detection (ex: 340 nm and em: 445 nm).…”

Fast simultaneous determination of free and conjugated isoflavones in soy milk by UHPLC–UV

“…Therefore, UHPLC is becoming a widely used new trend technique in separation science. Several new UHPLC methods for the determination of health-related compounds in various food matrices have recently been developed (Latorre-Moratalla et al, 2009;Nováková, Spáčil, Seifrtová, Opletal, & Solich, 2010). However, to our knowledge, no UHPLC method has been designed and validated for the determination of isoflavones in food.…”