Validation of an LC–MS/MS method for the determination of epirubicin in human serum of patients undergoing Drug Eluting Microsphere-Transarterial Chemoembolization (DEM-TACE)

Characterization and identification of pradimicin analogs from Actinomadura hibisca using liquid chromatography–tandem mass spectrometry

“…As expected from previous studies, glycosidic bond cleavage was the main fragmentation pattern; a product ion observed for IS at m/z 130 corresponds to a protonated aminosugar moiety l-daunosamine [19][20][21], whereas a fragment ion for authentic PRA at m/z 292 matches to a protonated Xylattached Tho. Most therapeutic drug monitoring studies of MS/MS transitions of authentic IS using ESI-interfaced ion trap-and tandem-mass spectrometers show that the dominant ion was assigned at m/z 130, identical to the results of this work [20,21]. Therefore, the appearance of specific fragmented ions can be used to identify the analyte; and the characteristic mass transition between the protonated precursor and the product ion can be used to quantify and complete the metabolite profiling using HPLC-ESI-MS/MS in MRM mode.…”

Quantitative liquid chromatographic analysis of anthracyclines in biological fluids

“…In an attempt to overcome their toxicity or drug resistance, prodrugs and special pharmaceutical formulations have been developed. Since these changes often require a different analytical approach, the interested reader is referred to the individual methods regarding the analysis of peptide-conjugated [29][30][31] or polymer-bound [32] prodrugs and micellar [33], pegylated liposomal [33][34][35], liposomal [36,37] or embolizing [38][39][40] formulations. Here we present an overview of 35 original methods published since 1990 for the determination of doxorubicin, epirubicin, daunorubicin, idarubicin and metabolites in biological fluids.…”

Confirmation of drug delivery after liver chemoembolization: direct tissue doxorubicin measurement by UHPLC‐MS‐MS

“…This investigation addressed the use of UHPLC‐MS‐MS for validation of drug delivery after transcatheter arterial chemoembolization in rabbit VX2 tumor HCC, which has not been applied previously in studies of liver chemoembolization. Although previous studies have used LC‐MS‐MS to quantify concentrations of chemotherapy drugs in serum (Arnold et al ., ; Sottani et al ., ) and liver tissue (Arnold et al ., ), we extended this approach to include the faster technique of UHPLC‐MS‐MS for the measurement of doxorubicin uptake in tissue after chemoembolization. In the UHPLC‐MS‐MS chromatogram shown in Fig.…”