Validation of an Electrospray Ionization LC-MS/MS Method for Quantitative Analysis of Raltegravir, Etravirine, and 9 Other Antiretroviral Agents in Human Plasma Samples

Abstract

A rapid, specific, and sensitive LC-MS/MS method for quantification of raltegravir, etravirine, and 9 other antiretrovirals in human plasma was developed and was successfully applied for routine therapeutic drug monitoring.

High Performance Liquid Chromatography Using UV Detection for the Simultaneous Quantification of the New Non-nucleoside Reverse Transcriptase Inhibitor Etravirine (TMC-125), and 4 Protease Inhibitors in Human Plasma

“…This method is simple, reliable, and sensitive, using cost-effective instrumentation when compared with others. [5][6][7] However, this method requires a solid phase extraction. Furthermore, in general hospitals, a UV detector coupled with HPLC is more popular than a PDA detector.…”

“…Fayet et al 5) and Quaranta et al 6) succeeded in determining plasma concentrations of ETV and other drugs through the use of liquid chromatography-tandem mass spectrometry (LC-MS/MS). Rezk et al 7) have also developed a method to measure plasma concentrations of ETV and PIs by LC-MS. LC-MS or LC-MS/MS assay is very sensitive and accurate.…”

Spectrophotometric Determination of Etravirine in Bulk and Pharmaceutical Formulations

“…A detailed literature survey for Etravirine revealed that various methods based on varied techniques namely LC-MS/MS [5][6][7][8], HPLC-MS [9,10], HPLC [11,12], and a direct UV-Visible spectrophotometric method for validation of Etravirine at 310 nm performed with equal ratio of acetonitrile and water as solvent, were reported [13]. The experimental results were applied for statistical analysis and found satisfactory precision and reproducibility.…”

Simultaneous LC-MS/MS Determination of Racemic Warfarin and Etravirine in Rat Plasma and Its Application in Pharmacokinetic Studies

“…For example, (Heine et al, 2009) developed an LC-MS/MS method quantifying etravirine in human plasma, dry blood spot, and peripheral blood mononuclear cell lysate. LC-tandem MS methods were also available for simultaneous analysis of etravirine with other NNRTIs (Fayet et al, 2009;Else et al, 2010) in human plasma and with protease inhibitors (Quaranta et al, 2009). All the above referenced methods used a direct plasma protein precipitation using organic co-solvent and had etravirine detection limit of > 10 ng/mL, except for a study by (Rezk et al, 2009), where a 2 ng/mL detection limit was reported.…”