2009 Help me understand this report
Abstract: Chlamydophila abortus (C. abortus) is associated with reproductive problems in cattle, sheep, and goats. Diagnosis of C. abortus using embryonated chicken eggs or immortalized cell lines has a very low sensitivity. Polymerase chain reaction (PCR) assays have been used to detect C. abortus infection in clinical specimens
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2009
2009
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Cited by 2 publications
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References 28 publications
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“…In order to identify C. abortus in the evaluated specimens, a PCR assay that amplifies an 856-bp product from the intergenic region between the 16S and 23S rRNA genes was carried out according to Silva-Zacarias et al (2009a) o C. Additionally, the efficiencies of the nucleic acid extraction and DNA amplification procedures for the bovine abortion samples were evaluated by a PCR assay employing an internal control. These reactions were carried out with primers BOV1 (5'-ATACGCCTTCATTACCAG-3') and BOV2 (5'-TTGAATGGAGTAGTGCTG-3'), which amplify a 626-bp amplicon from the ND5 gene of bovine mitochondrial DNA (Wosiacki et al, 2005).…”
Section: Pcr Assaymentioning
confidence: 99%
“…In order to identify C. abortus in the evaluated specimens, a PCR assay that amplifies an 856-bp product from the intergenic region between the 16S and 23S rRNA genes was carried out according to Silva-Zacarias et al (2009a) o C. Additionally, the efficiencies of the nucleic acid extraction and DNA amplification procedures for the bovine abortion samples were evaluated by a PCR assay employing an internal control. These reactions were carried out with primers BOV1 (5'-ATACGCCTTCATTACCAG-3') and BOV2 (5'-TTGAATGGAGTAGTGCTG-3'), which amplify a 626-bp amplicon from the ND5 gene of bovine mitochondrial DNA (Wosiacki et al, 2005).…”
Section: Pcr Assaymentioning
confidence: 99%
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