Validation of a method for the simultaneous quantification of chloroquine, desethylchloroquine and primaquine in plasma by HPLC-DAD

An HPLC method with diode array detector for the simultaneous quantification of chloroquine and desethylchloroquine in plasma and whole blood samples from Plasmodium vivax patients in Vietnam, using quinine as an internal standard

“…In general, our results in terms of LOD, LOQ were comparable and more sensitive than those reported in a number of previous publications (Chaulet et al , 1993, 1994; Cheomung and Na‐Bangchang, 2011; Dua et al , 1999; Lejeune et al , 2007; Yakasai, 2006; Zuluaga‐Idárraga et al , 2014). Moreover, it was successfully achieved with a smaller sample volume of 100 μL vs. 150 μL (Cheomung and Na‐Bangchang, 2011), 200 μL (Bustos et al , 2002), 500 μL (Dua et al , 1999; Zuluaga‐Idárraga et al , 2014) and 1000 μL (Chaulet et al , 1994; Deng et al , 2006; Karunajeewa et al , 2008, 2010; Yakasai, 2006) in previous studies.…”

“…Moreover, it was successfully achieved with a smaller sample volume of 100 μL vs. 150 μL (Cheomung and Na‐Bangchang, 2011), 200 μL (Bustos et al , 2002), 500 μL (Dua et al , 1999; Zuluaga‐Idárraga et al , 2014) and 1000 μL (Chaulet et al , 1994; Deng et al , 2006; Karunajeewa et al , 2008, 2010; Yakasai, 2006) in previous studies. However, when CQ and MCQ analysis was performed by LC‐MS/MS, the LOQ was 7.5 ng/mL for CQ using 200 μL plasma (Hodel et al , 2009), 0.5 ng/mL for MCQ and 1.0 ng/mL for CQ (Tang and Sojinu, 2012), and 0.2 ng/mL for CQ and 0.4 ng/mL for MCQ using 100 μL plasma (Boonprasert et al , 2012).…”

A newly validated high-performance liquid chromatography method with diode array ultraviolet detection for analysis of the antimalarial drug primaquine in the blood plasma

“…In fact, LOQs reported in the literature [12][13][14][16][17][18] for PQ analyses using HPLC-UV are up to 10ng.mL -1 . The excellent signal symmetry and linearity across a range of concentrations obtained using the new method led to markedly lower LOQ and LOD estimates than those previously reported for similar HPLC methods for PQ.…”

“…With respect to the stationary phase, most methods use reversed phase C18 columns with different lengths, internal diameters, and particle sizes, while a few methods employ other silicamodified stationary phases, such as phenyl and cyanopropyl. All methods require large sample volumes (≈500µL of plasma), thereby limiting their use in studies of small rodents, such as mice and hamsters ( Table 1) [12][13][14][15][16][17][18][19] . The long retention times of PQ imply relatively long analysis times (≥8 min) for these chromatographic methods.…”

Development and Validation of an Hilic–MS/MS Method by One-Step Precipitation for Chloroquine in Miniature Pig Plasma

“…Although many analytical methods have been developed for the detection of chloroquine, most of these use expensive solid phase extraction (SPE) columns [7][8][9] or require time-consuming liquid-liquid extractions (LLE) combined with extra preacidification operation [10][11][12] and/or prealkalization [10][11][12][13] procedure and then processed to an ultraviolet detector [7][8][9][10][11][12][13]. Brief summary of existing HPLC methods for the analysis Development and validation of an HILIC-MS/MS method by one-step precipitation for chloroquine in miniature pig plasma future science group Methodology Wang, Lu, Shang, Ni, Zhang & Wen Table 1.…”