Validation of a male-specific DNA marker confirms XX/XY-type sex determination in several Hungarian strains of African catfish (Clarias gariepinus)

Balogh, Réka Enikő; Csorbai, Balázs; Guti, Csaba Ferenc; Keszte, Szilvia; Urbányi, Béla; Orbán, László; Kovács, Balázs

doi:10.1016/j.theriogenology.2023.04.017

Cited by 5 publications

(2 citation statements)

References 68 publications

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“…Moreover, ZZ/ZW sex determination system, which is likely the ancestral system for the North African catfish, is the same as the systems reported in Africa and Israel. However, studies on different populations from Israel, Hungary, and China have suggested the presence of an XX/XY system or the potential coexistence of both sex chromosome systems, indicating multiple systems of sex determination in the North African catfish [74][75][76][77][78][79][80][81][82][83]. This suggests that comprehensive genomic analyses of diverse populations derived from different regions are required to address the taxonomic issues in the North African catfish.…”

Section: Possible Diverse Genetic Origins Of the North African Catfis...mentioning

confidence: 99%

Questioning inbreeding: Could outbreeding affect productivity in the North African catfish in Thailand?

Patta,

Panthum,

Thatukan

et al. 2024

PLoS ONE

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The North African catfish (Clarias gariepinus) is a significant species in aquaculture, which is crucial for ensuring food and nutrition security. Their high adaptability to diverse environments has led to an increase in the number of farms that are available for their production. However, long-term closed breeding adversely affects their reproductive performance, leading to a decrease in production efficiency. This is possibly caused by inbreeding depression. To investigate the root cause of this issue, the genetic diversity of captive North African catfish populations was assessed in this study. Microsatellite genotyping and mitochondrial DNA D-loop sequencing were applied to 136 catfish specimens, collected from three populations captured for breeding in Thailand. Interestingly, extremely low inbreeding coefficients were obtained within each population, and distinct genetic diversity was observed among the three populations, indicating that their genetic origins are markedly different. This suggests that outbreeding depression by genetic admixture among currently captured populations of different origins may account for the low productivity of the North African catfish in Thailand. Genetic improvement of the North African catfish populations is required by introducing new populations whose origins are clearly known. This strategy should be systematically integrated into breeding programs to establish an ideal founder stock for selective breeding.

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Section: Possible Diverse Genetic Origins Of the North African Catfis...mentioning

confidence: 99%

Questioning inbreeding: Could outbreeding affect productivity in the North African catfish in Thailand?

Patta,

Panthum,

Thatukan

et al. 2024

PLoS ONE

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show abstract

“…To identify the putative paternal haplotype in our assemblies, the full-length nucleotide sequences of two previously identified and validated male-specific DNA markers (Accession numbers: CgaY1: AF332597; CgaY2: AF332598) were obtained from GenBank 29,55 . Both sequences, 2.6 kb (CgaY1) and 458 bp (CgaY2) in length, were BLASTed against Hap1, Hap2, and Prim assemblies, requiring stringent mapping criteria (identity >98%; queryCoverage >98%).…”

Section: Identification Of the Putative Sex-specific Haplotypementioning

confidence: 99%

Haplotype-resolved assembly of the African catfish (Clarias gariepinus) provides insights for semi-terrestrial adaptation of airbreathing catfishes

Nguinkal

Zoclanclounon

Brunner

et al. 2023

Preprint

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Airbreathing catfishes (clariids) are a group of stenohaline freshwater fish that can withstand various environmental conditions and farming practices, including the ability to breathe atmospheric oxygen. This unique ability has allowed them to thrive in semi-terrestrial habitats. However, the underlying genomic and adaptive mechanisms remain poorly investigated. Here, we sequenced the genome of the African catfish Clarias gariepinus, one of the most commonly farmed clariids, and generated a gapless telomere-to-telomere (T2T) chromosome-level assembly with high-resolution haplotypes, by integrating long-range sequencing (Hi-C) with PacBio single-molecule (HiFi), Oxford Nanopore, and Illumina sequencing data. The diploid genome assembly yielded 58 contigs with a total length of 969.72 Mb and a contig N50 of 33.71 Mb. We report 25,655 predicted protein-coding genes and 49.94% repetitive elements in the African catfish genome. Our genome assembly provides the first comprehensive gene annotation and haplotype information, such as the male-specific haplotype, enabling us to identify putative genes and molecular mechanisms underlying amphibious traits and terrestrial adaptation of airbreathing catfishes. Several gene families involved in ion transport, osmoregulation, oxidative stress response, and muscle metabolism were expanded or positively selected in clariids, suggesting a potential role in their transition to terrestrial life. The reported findings expand our understanding of the genomic mechanisms underpinning the resilience and adaptive mechanisms of C. gariepinus to adverse ecological conditions. They will serve as a valuable resource for future studies in elucidating these unique biological traits in related teleosts and leverage these insights for aquaculture improvement.

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Pattern of color inheritance in African catfish (Clarias gariepinus): an expression of a Mendelian law

Nwachi,

Irabor,

Umehai

et al. 2023

Fish Physiol Biochem

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Validation of a male-specific DNA marker confirms XX/XY-type sex determination in several Hungarian strains of African catfish (Clarias gariepinus)

Cited by 5 publications

References 68 publications

Questioning inbreeding: Could outbreeding affect productivity in the North African catfish in Thailand?

Questioning inbreeding: Could outbreeding affect productivity in the North African catfish in Thailand?

Haplotype-resolved assembly of the African catfish (Clarias gariepinus) provides insights for semi-terrestrial adaptation of airbreathing catfishes

Pattern of color inheritance in African catfish (Clarias gariepinus): an expression of a Mendelian law

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