Validated Stability Indicating Rp-HPLC Method for the Estimation of Linezolid in a Pharmaceutical Dosage Form

Mohapatra, Sharmistha; Annapurna, Mukthinuthalapati Mathrusri; Kumar, B. V. V. Ravi; Anwar, Mohammed; Warsi, Musarrat Husain; Akhter, Sohail

doi:10.1080/10826076.2011.585548

Cited by 19 publications

(12 citation statements)

References 18 publications

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“…The results shown in Table 5 are in good agreement with those obtained using the comparison methods. 4,30 Statistical analysis of the results obtained using Student's t-test and variance ratio F-test 38 revealed no signicant difference between the performance of 2,40 This characteristic is a major benet, allowing this agent to initially be used intravenously, followed by switching to oral routes, or indeed even to commence treatment of infection with oral therapy. Aer oral doses of 600 mg, steadystate peak serum concentrations (C max ) are 15-27 mg mL À1 and are reached 0.5-2 h aer administration.…”

Section: Validation Of the Methodsmentioning

confidence: 95%

“…To prove the accuracy of the proposed method, the results of the assay of the studied drugs were compared with those of the comparison methods. 4,30 Statistical analysis of the results using Student's t-test and variance ratio F-test 38 revealed no signicant difference between the performance of the two methods regarding the accuracy and precision, respectively (Table 3). Both the official method 4 for RIF and the comparison method for LNZ are HPLC methods.…”

Section: Validation Of the Methodsmentioning

confidence: 98%

“…The purity percentage of LNZ was 99.95 AE 1.01, as found by applying the comparison method. 30 RIF was kindly provided by Hebei Xingang Pharmaceutical Co., Ltd, lot #C07B1206023. The purity percentage of RIF was 100.04 AE 0.93, as found by applying the USP official method.…”

Section: Materials and Reagentsmentioning

confidence: 99%

“…7 Several analytical methods have been developed for the determination of RIF in biological uids and pharmaceutical dosage forms, including methods based on HPTLC, 8 HPLC, [9][10][11][12][13][14][15][16][17] HPLC following SPE 18 or SBSE, 19 UPLC 20 and LC-MS/MS. [21][22][23][24] Regarding LNZ, it is not yet official in any of the pharmacopoeia, but several methods have been reported for its determination, such as spectrophotometry, [25][26][27] HPLC, [28][29][30] TLC, 31 capillary electrophoresis 32 and electrochemical analysis. 33 Trials have shown additive benets when combining LNZ with RIF for the treatment of MRSA infection and decreased development of RIF resistance.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Micellar HPLC method using monolithic column for the simultaneous determination of linezolid and rifampicin in pharmaceuticals and biological fluids

et al. 2013

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Section: Validation Of the Methodsmentioning

confidence: 95%

Section: Validation Of the Methodsmentioning

confidence: 98%

Section: Materials and Reagentsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Micellar HPLC method using monolithic column for the simultaneous determination of linezolid and rifampicin in pharmaceuticals and biological fluids

et al. 2013

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“…Only few methods were reported for the determination of LIN in tablets, including spetrophotometric (18), TLC (19) and HPLC (20) methods, either alone or in the presence of alkaline induced degradation products. Estimation of LIN in biological fluids using LC/MS (21) and HPLC (22) was also described.…”

mentioning

confidence: 99%

Spectrofluorimetric determination of gemifloxacin mesylate and linezolid in pharmaceutical formulations: Application of quinone-based fluorophores and enhanced native fluorescence

Moussa¹,

Mahrouse²,

Hassan³

et al. 2014

Acta Pharmaceutica

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Quinone-based fluorophores and enhanced native fluorescence techniques were applied for a fast quantitative analysis of gemifloxacin mesylate (GEM) and linezolid (LIN) in pharmaceutical formulations. For this purpose, three sensitive, accurate and precise spectrofluorimetric methods were developed. GEM, as an n-electron donor, reacts with 7,7,8,8-tetracyanoquinodimethane (method A) and 2,5-dichloro-3,6-dihydroxy-p-benzoquinone (method B) as п-electron acceptors, forming charge transfer complexes that exhibit high fluorescence intensity at 441 and 390 nm upon excitation at 260 and 339 nm, respectively. Method C depends on measurement of enhanced native fluorescence of LIN in phosphate buffer (pH 5) at 380 nm upon excitation at 260 nm. Experimental factors affecting fluorescence intensity were optimized. Linearity was obtained over concentration ranges 50-500, 10-60 and 20-400 ng mL-1 for methods A, B and C, respectively. The developed methods were validated and successfully applied for determination of the cited drugs in tablets.

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Spectrofluorimetric Determination of Terbinafine Hydrochloride and Linezolid in their Dosage Forms and Human Plasma

et al. 2013

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A highly sensitive, simple and rapid spectrofluorimetric method was developed for the determination of Terbinafine HCl (TRH) and linezolid (LNZ) in their pharmaceutical formulations. The proposed method is based on measuring the native fluorescence of the studied drugs in water at 336 nm after excitation at 275 nm for TRH and 375 nm after excitation at 254 nm for LNZ. The fluorescence-concentration plots were rectilinear over the range of 0.02-0.15 μg/mL for TRH and 0.5-5.0 μg/mL for LNZ. With lower detection limits of 3.0 and 110.0 ng/mL and a lower quantification limit of 9.0 and 320.0 ng/mL for TRH and LNZ, respectively. The method was successfully applied to the analysis of TRH in its commercial tablets, cream, gel and spray formulations and the results were in good agreement with those obtained with the official method. In addition the method was also applied to the analysis of LNZ in its capsule and I.V solution and the results were in good agreement with those obtained with the comparison method. The effect of sensitizers was studied. The method was extended to the determination of the studied drugs in spiked human plasma and the results were satisfactory.

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Validated Stability Indicating Rp-HPLC Method for the Estimation of Linezolid in a Pharmaceutical Dosage Form

Cited by 19 publications

References 18 publications

Micellar HPLC method using monolithic column for the simultaneous determination of linezolid and rifampicin in pharmaceuticals and biological fluids

Micellar HPLC method using monolithic column for the simultaneous determination of linezolid and rifampicin in pharmaceuticals and biological fluids

Spectrofluorimetric determination of gemifloxacin mesylate and linezolid in pharmaceutical formulations: Application of quinone-based fluorophores and enhanced native fluorescence

Spectrofluorimetric Determination of Terbinafine Hydrochloride and Linezolid in their Dosage Forms and Human Plasma

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