Validação interlaboratorial do teste de polarização fluorescente para o diagnóstico sorológico da brucelose bovina

Mathias, Luís Antônio; Corbellini, Luís Gustavo; Maia, L. R.; Nascimento, Kelly Fagundes; Paulin, L.; Samartino, Luis; Serqueira, Marco Antonio; Filho, Paulo Martins Soares; Souza, Marcília Maria Alves de

doi:10.1590/s0103-84782010005000161

Cited by 5 publications

(4 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The agreement of the serological tests used in cattle was median. This difference was mainly due to the sensitivity and specificity of the tests, resulting in a higher prevalence when using the FPA test because it is more sensitive and corroborating with the studies carried out by Nielsen et al (1999) and Mathias et al (2010).…”

Section: Resultssupporting

confidence: 76%

Prevalência sorológica de Brucella spp. em porcos ferais e bovinos em simpatria no Pantanal do Mato Grosso do Sul, Brasil

Zimmermann

Péres

Braz

et al. 2018

SCA

View full text Add to dashboard Cite

The objective of this study was to determine the prevalence of anti-Brucella antibodies in feral pigs and bovines simpatrics in the Pantanal subregions of Paiaguás and Nhecolândia. The study was conducted in the municipality of Corumbá, Mato Grosso do Sul State, Brazil. A total of 105 feral pigs and 256 cattle were sampled in 12 farms, in all animals blood samples were collected for the serological diagnosis with Rose Bengal Test (RBT) for screening, 2-Mercaptoethanol (2-ME) confirmatory test and comparative test with Fluorescence Polarization Assay (FPA). The prevalence of positive feral pigs were 1% (1/105) in the RBT and FPA and no positive AAT results were confirmed in the 2-ME test. The prevalence of positive cattle sampled was 11.32%, 4.3% and 7.42% in the RBT, 2-ME and FPA tests respectively. The degree of agreement obtained between the serological tests used in cattle was Kappa = 0.506 (p <0.001), 95% CI (0.282 - 0.729). The results of the serological tests demonstrated that brucellosis is widespread in bovine herds of the region studied, but the same type of exposure to the agent did not occur in feral pigs according to the diagnostic tests used.

show abstract

Section: Resultssupporting

confidence: 76%

Prevalência sorológica de Brucella spp. em porcos ferais e bovinos em simpatria no Pantanal do Mato Grosso do Sul, Brasil

Zimmermann

Péres

Braz

et al. 2018

SCA

View full text Add to dashboard Cite

show abstract

“…Foi feita também a interpretação usando um ponto de corte fixo. Para isso, foi adotado o valor de 87,3 mP (MATHIAS et al, 2010).…”

Section: Methodsunclassified

RESPOSTA SOROLÓGICA DE BEZERRAS TABAPUÃS VACINADAS COM CEPA B19 de Brucella abortus

Blankenheim

Silva

Santos

et al. 2015

Ars Vet

View full text Add to dashboard Cite

RESUMOFoi avaliada a resposta sorológica induzida pela vacinação com a dose padrão de 60-120x10 9 organismos da cepa B19 de Brucella abortus em bezerras Tabapuãs, entre 3 e 8 meses de idade, pelos testes sorológicos preconizados pelo Programa Nacional de Controle e Erradicação da Brucelose e Tuberculose (PNCEBT) para diagnóstico da brucelose bovina. Foram examinadas amostras de soro sanguíneo de 72 animais em quatro provas sorológicas: antígeno acidificado tamponado (AAT), teste de soroaglutinação lenta juntamente com o teste de 2-mercaptoetanol (2-ME), reação de fixação de complemento (RFC) e teste de polarização fluorescente (TPF), o qual foi interpretado de duas formas: ponto de corte variável e ponto de corte fixo. As amostras de soro sanguíneo foram colhidas imediatamente antes da vacinação, e após 30, 60, 90, 120, 150, 180, 210, 240, 270, 300, 330 e 360 dias. Foi calculada a especificidade dos testes e os resultados foram comparados pelo indicador kappa. O TPF apresentou especificidade mais elevada do que os outros testes nas amostras colhidas entre 30 e 90 dias após a vacinação. Aos 270 dias de vacinação, observou-se especificidade de 93,74% no AAT, no 2-ME e na RFC. No TPF com ponto de corte variável a especificidade foi 87,5% e com ponto de corte fixo foi 100,0%. O TPF apresentou maior capacidade de discriminação dos títulos de anticorpos vacinais logo após a vacinação, quando comparado com os outros testes preconizados pelo PNCEBT, mostrando-se um teste útil para o diagnóstico da brucelose bovina. PALAVRAS-CHAVE:Brucelose, Polarização Fluorescente, Sorologia, Vacinação. SUMMARYThe study aimed to analyze the antibody response induced by vaccination with the standard dose of 60-120x10 9 organisms of Brucella abortus strain 19 in Tabapuã heifers, aged between 3 and 8 months, by serological tests recommended by the National Program for Brucellosis and Tuberculosis Control and Eradication (PNCEBT) for the diagnosis of bovine brucellosis. Serum samples of 72 animals were examined by four serological tests: rose Bengal test (RBT), standard agglutination test with the 2-mercaptoetanol (2-ME) test, complement fixation test (CFT) and fluorescence polarization assay (FPA), which was interpreted in two ways: variable cutoff and fixed cutoff. Heifers were bled immediately prior to vaccination, and after 30, 60, 90, 120, 150, 180, 210, 240, 270, 300, 330 and 360 days. Specificity of the tests was calculated, and the results were compared using the kappa statistic. FPA had a higher specificity than the other tests on the samples collected from 30 to 90 days after vaccination, but over time this difference decreased. At 270 days of vaccination, the specificity of RBT, 2-ME and RFC was 93.74%. The specificity of the FPA with variable cutoff was 87.5%, and with fixed cutoff was 100.0%. It was concluded that FPA had a higher discrimination ability for vaccination antibody titers shortly after vaccination, when compared with other tests recommended by the PNCEBT, and is a useful tool for the diagnosis of bovine brucellos...

show abstract

“…According to MARTIN et al (1987) andMATHIAS et al (2010), the gold standard was made up of the combination of CFT and 2-ME. Animals classified as positive in both CFT (titer =20IU) and 2-ME (not vaccinated criteria) were considered to be positive gold standards and negative in both, CFT (titer <20IU) and 2-ME (not vaccinated criteria), were considered to be negative gold standards.…”

Section: Serummentioning

confidence: 99%

“…Disadvantages are related to the initial investment in the equipment and the impossibility, as in the other tests, of differentiating vaccinated from infected animals. Furthermore, if the process is not automated, it will take longer than AAT (MATHIAS, 2010).…”

Section: Introductionmentioning

confidence: 99%

Fluorescence polarization assay, competitive enzyme-linked immunosorbent assay (ELISA-C) and indirect ELISA for the diagnosis of brucellosis in buffaloes (Bubalus bubalis)

et al. 2012

Self Cite

View full text Add to dashboard Cite

The objective of the present study was to compare the performance of three serological tests for diagnosis of Brucella abortus infections in buffaloes (Bubalus bubalis). Serum samples collected from 696 adult females were submitted to the competitive enzyme-linked immunosorbent assay (ELISA-C), (I-ELISA), fluorescence polarization test (FPA), 2-mercaptoethanol test (2-ME) and complement fixation test (CFT). The gold standard was the combination of CFT and 2-ME, considering as positive the reactors in both CFT and 2-ME, and as negative those non-reactors. ROC analyses were done for C-ELISA, I-ELISA and FPA and the Kappa agreement index were also calculated. The best combinations of relative sensitivity (SEr) and relative specificity (SPr) and Kappa were given by C-ELISA (96.9%, 99.1%, and 0.932, respectively) and FPA (92.2%, 97.6 and 0.836, respectively). The C-ELISA and FPA were the most promising confirmatory tests for the serological diagnosis of brucellosis in buffaloes, and for these tests, cut-off values for buffaloes may be the same as those used for bovines.

show abstract

Validação interlaboratorial do teste de polarização fluorescente para o diagnóstico sorológico da brucelose bovina

Abstract: Validação interlaboratorial do teste de polarização fluorescente para o diagnóstico sorológico da brucelose bovinaInterlaboratorial validation of the fluorescence polarization assay for the serodiagnosis of bovine brucellosisRecebido para publicação 11.01

Cited by 5 publications

References 11 publications

Prevalência sorológica de Brucella spp. em porcos ferais e bovinos em simpatria no Pantanal do Mato Grosso do Sul, Brasil

Prevalência sorológica de Brucella spp. em porcos ferais e bovinos em simpatria no Pantanal do Mato Grosso do Sul, Brasil

RESPOSTA SOROLÓGICA DE BEZERRAS TABAPUÃS VACINADAS COM CEPA B19 de Brucella abortus

Fluorescence polarization assay, competitive enzyme-linked immunosorbent assay (ELISA-C) and indirect ELISA for the diagnosis of brucellosis in buffaloes (Bubalus bubalis)

Contact Info

Product

Resources

About