1992
DOI: 10.1590/s0074-02761992000900001 View full text |Buy / Rent full text
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Abstract: Schistosomiasis, the second major parasitic disease in the world after malaria affects at least 200 million people, 500 million being exposed to the risk of infection. It is widely agreed that a vaccine strategy which could lead to the induction of effector mechanisms reducing the level of reinfection and ideally parasite fecundity would deeply affect the incidence of pathological manifestations as well as the parasite transmission potentialities. Extensive studies performed in the rat model have allowed the i… Show more

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“…In S. mansoni infection, high levels of IgE were associated with resistance, whereas high levels of IgG4 antibodies were associated with increased susceptibility. 44 IgG4 is thought to compete with IgE for the same parasite epitopes in both schistosomiasis and filariasis. 45 The pattern of IgG4 reactivity observed here, like that of IgE, was much greater in the crusted scabies patients.…”
Section: Discussionmentioning
“…Endotoxins were removed from the Fh12 by using polymyxin B (PMB)-columns (31) according to the manufacturer’s instructions. The presence of endotoxins was assessed prior to and after removing endotoxins using the Chromogenic Limulus Amebocyte Lysate (LAL) QCL-1000 Assay (Lonza, Walkersville, MD, USA) following the manufacturer’s instructions.…”
Section: Methodsmentioning
“…Cells were incubated in the extraction buffer on ice using a rocking platform for 30 min before being centrifuged at 20,000 × g for 10 min at 4°C. Supernatants were transferred to clean tubes, and protein concentrations were determined using a BCA protein assay kit (31). …”
Section: Methodsmentioning
“…These biological functions make GSTs molecular targets for new anti®larial drugs (Brophy and Pritchard 1994). GST isozymes have shown potential as protective antigens against fascioliasis and schistosomiasis (Balloul et al 1987;Mitchell 1989;Sexton et al 1990;Boulanger et al 1991;Capron et al 1992;Xu et al 1993).…”
Section: Introductionmentioning
“…A variety of immunodominant molecules have been described as candidate vaccines against schistosomiasis, since they induce a substantial degree of protection (reviewed by Bergquist [6]). Among these partially protecting schistosome antigens are the following: glutathione S-transferase (GST) (Sm28 GST) (7,8,21), the muscle protein paramyosin (Sm97) (22), the irradiation-associated vaccine antigen (IrV-5) (27,28), triose-phosphate isomerase (9,23), the membrane antigen Sm23 (24), and fatty acid binding protein 14 (Sm14) (35). These candidate vaccine antigens are either ubiquitous enzymes usually involved in metabolic pathways, muscle proteins, or surface antigens, and they induced 30 to 60% protection against challenge infection.…”
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