UVB-induced apoptosis and DNA damaging potential of chrysene via reactive oxygen species in human keratinocytes

Ali, Daoud; Verma, Ankit; Mujtaba, F.; Dwivedi, Ashish; Hans, R.K.; Ray, Ratan Singh

doi:10.1016/j.toxlet.2011.04.033

Cited by 54 publications

(24 citation statements)

References 41 publications

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“…A number of recent studies show that UVB light induces apoptosis in keratinocytes [24,25,26]. In keeping with this observation, intact nuclei were observed in control, and cells treated only with BHE, whereas significant nuclear fragmentation (characteristic of apoptotic body formation) was observed in UVB-irradiated cells (apoptotic index, 19.3).…”

Section: Resultssupporting

confidence: 68%

An Ethanol Extract Derived from Bonnemaisonia hamifera Scavenges Ultraviolet B (UVB) Radiation-Induced Reactive Oxygen Species and Attenuates UVB-Induced Cell Damage in Human Keratinocytes

et al. 2012

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The present study investigated the photoprotective properties of an ethanol extract derived from the red alga Bonnemaisonia hamifera against ultraviolet B (UVB)-induced cell damage in human HaCaT keratinocytes. The Bonnemaisonia hamifera ethanol extract (BHE) scavenged the superoxide anion generated by the xanthine/xanthine oxidase system and the hydroxyl radical generated by the Fenton reaction (FeSO4 + H2O2), both of which were detected by using electron spin resonance spectrometry. In addition, BHE exhibited scavenging activity against the 1,1-diphenyl-2-picrylhydrazyl radical and intracellular reactive oxygen species (ROS) that were induced by either hydrogen peroxide or UVB radiation. BHE reduced UVB-induced apoptosis, as shown by decreased apoptotic body formation and DNA fragmentation. BHE also attenuated DNA damage and the elevated levels of 8-isoprostane and protein carbonyls resulting from UVB-mediated oxidative stress. Furthermore, BHE absorbed electromagnetic radiation in the UVB range (280–320 nm). These results suggest that BHE protects human HaCaT keratinocytes against UVB-induced oxidative damage by scavenging ROS and absorbing UVB photons, thereby reducing injury to cellular components.

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Section: Resultssupporting

confidence: 68%

An Ethanol Extract Derived from Bonnemaisonia hamifera Scavenges Ultraviolet B (UVB) Radiation-Induced Reactive Oxygen Species and Attenuates UVB-Induced Cell Damage in Human Keratinocytes

et al. 2012

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“…These conditions exacerbate degradation of the ozone layer, leading to exposure of the earth’s surface to sunlight containing a large amount of UVB radiation (Bais et al ., 1993; McKenzie et al ., 2011). Although UVB radiation has beneficial effects, such as vitamin D synthesis, on organisms, exposure to excessive amounts of UVB radiation can cause cellular damage and cell death (Drouin and Therrien, 1997; Ali et al ., 2011; Wacker and Holick, 2013). In particular, upon exposure to excessive amounts of UVB radiation, skin cells can undergo death and skin cancer often develops (Drouin and Therrien, 1997; Ali et al ., 2011).…”

Section: Discussionmentioning

confidence: 99%

“…Although UVB radiation has beneficial effects, such as vitamin D synthesis, on organisms, exposure to excessive amounts of UVB radiation can cause cellular damage and cell death (Drouin and Therrien, 1997; Ali et al ., 2011; Wacker and Holick, 2013). In particular, upon exposure to excessive amounts of UVB radiation, skin cells can undergo death and skin cancer often develops (Drouin and Therrien, 1997; Ali et al ., 2011). These effects of UVB radiation are due to the induction of DNA damage and oxidative stress via ROS generation in skin cells (Ravanat et al ., 2001; Fagot et al ., 2002; Ichihashi et al ., 2003).…”

Section: Discussionmentioning

confidence: 99%

Fucodiphlorethol G Purified from Ecklonia cava Suppresses Ultraviolet B Radiation-Induced Oxidative Stress and Cellular Damage

Kim¹,

Piao

Zheng

et al. 2014

Biomolecules & Therapeutics

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Fucodiphlorethol G (6’-[2,4-dihydroxy-6-(2,4,6-trihydroxyphenoxy)phenoxy]biphenyl-2,2’,4,4’,6-pentol) is a compound purified from Ecklonia cava, a brown alga that is widely distributed offshore of Jeju Island. This study investigated the protective effects of fucodiphlorethol G against oxidative damage-mediated apoptosis induced by ultraviolet B (UVB) irradiation. Fucodiphlorethol G attenuated the generation of 2, 2-diphenyl-1-picrylhydrazyl radicals and intracellular reactive oxygen species in response to UVB irradiation. Fucodiphlorethol G suppressed the inhibition of human keratinocyte growth by UVB irradiation. Additionally, the wavelength of light absorbed by fucodiphlorethol G was close to the UVB spectrum. Fucodiphlorethol G reduced UVB radiation-induced 8-isoprostane generation and DNA fragmentation in human keratinocytes. Moreover, fucodiphlorethol G reduced UVB radiation-induced loss of mitochondrial membrane potential, generation of apoptotic cells, and active caspase-9 expression. Taken together, fucodiphlorethol G protected human keratinocytes against UVB radiation-induced cell damage and apoptosis by absorbing UVB radiation and scavenging reactive oxygen species.

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“…The alkaline single cell gel electrophoresis (SCGE) was performed as a three layer procedure [17]. The parameters used to measure DNA damage in cells were % tail DNA and Olive tail moment (OTM).…”

Section: Single Cell Gel Electrophoresis (Scge) Assaymentioning

confidence: 99%

Involvement of cathepsin B in mitochondrial apoptosis by p-phenylenediamine under ambient UV radiation

Goyal

Amar

Dubey

et al. 2015

Journal of Hazardous Materials

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UVB-induced apoptosis and DNA damaging potential of chrysene via reactive oxygen species in human keratinocytes

Cited by 54 publications

References 41 publications

An Ethanol Extract Derived from Bonnemaisonia hamifera Scavenges Ultraviolet B (UVB) Radiation-Induced Reactive Oxygen Species and Attenuates UVB-Induced Cell Damage in Human Keratinocytes

An Ethanol Extract Derived from Bonnemaisonia hamifera Scavenges Ultraviolet B (UVB) Radiation-Induced Reactive Oxygen Species and Attenuates UVB-Induced Cell Damage in Human Keratinocytes

Fucodiphlorethol G Purified from Ecklonia cava Suppresses Ultraviolet B Radiation-Induced Oxidative Stress and Cellular Damage

Involvement of cathepsin B in mitochondrial apoptosis by p-phenylenediamine under ambient UV radiation

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