UV-B, UV-A, and Blue Light Signal Transduction Pathways Interact Synergistically to Regulate Chalcone Synthase Gene Expression in Arabidopsis

Fuglevand, Geeta; Jackson, Jennie A.; Jenkins, Gareth I.

doi:10.2307/3870473

Cited by 52 publications

(96 citation statements)

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“…Soitamo et al, (2008) reported that light at low temperature induces the expression of genes involved in the synthesis of phenylpropanoids, carotenoids, and terpenoids. In Arabidopsis, the biosyntheses of light-dependent flavonoids and phenylpropanoids have been attributed to the upregulation of related genes at the transcript level and associated with an increase in primary metabolite production (Fuglevand et al, 1996;Hemm et al, 2004). The upregulation of GbMVD under light conditions possibly increases carbon partitioning towards terpenoid metabolism, resulting in increased TTL content in G. biloba.…”

Section: Discussionmentioning

confidence: 98%

Characterization and Transcriptional Profiling of Ginkgo biloba Mevalonate Diphosphate Decarboxylase Gene (GbMVD) Promoter Towards Light and Exogenous Hormone Treatments

Liao

Huang

et al. 2015

Plant Mol Biol Rep

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Mevalonate diphosphate decarboxylase (GbMVD) of Ginkgo biloba catalyzes the final step of mevalonic acid pathway by converting the six-carbon mevalonate diphosphate into isopentenyl diphosphate, a precursor of ginkgolides and bilobalide collectively termed terpene trilactones (TTLs). This paper presents the isolation of a 1.2-kb fragment (designated as GbMVDpro) of the 5′-flanking region of GbMVD. Extensive sequence analysis revealed the presence of evolutionarily conserved putative cis-acting elements in lightregulated transcription, hormone signaling (abscisic acid, jasmonate, and salicylic acid), and plant defense (W-box/ WRKY) in the GbMVD promoter region. Electrophoretic mobility shift analysis suggested possible involvement of W-box in GbMVD promoter function. To assess the organ-specific and environmentally responsive characteristics of GbMVD expression, GbMVDpro was fused to GUS reporter gene. GbMVDpro::GUS was introduced into tobacco. Histological analysis of the transgenic tobacco showed that GUS mainly localized in the leaf epidermis, stem secondary xylem, and root vasculature. These observations closely correlated with the previously reported presence of GbMVD transcripts in the roots, stems, and leaves. Further functional characterizations in transiently transformed tobacco leaves allowed us to identify the region that can be considered as the minimal promoter. Light and hormonal signals (i.e., ethephon, salicylic acid, and methyl jasmonate) induced the activity of GbMVDpro-driven GUS in transgenic tobacco and enhanced the transcription of GbMVD as well as TTL production in ginkgo seedlings. These results, together with the bioinformatic analysis of GbMVDpro, suggest that the GbMVD promoter harbored a numerous TTL biosynthesis-related cis-elements that regulate the flux of terpenoid precursors.

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Section: Discussionmentioning

confidence: 98%

Characterization and Transcriptional Profiling of Ginkgo biloba Mevalonate Diphosphate Decarboxylase Gene (GbMVD) Promoter Towards Light and Exogenous Hormone Treatments

Liao

Huang

et al. 2015

Plant Mol Biol Rep

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“…In a great variety of plant species the de-novo synthesis of CHS can be enhanced by irradiation with light, especially UV light . Dierent light qualities often interact synergistically (Fluglevand et al 1996) in stimulating¯avonoid accumulation. In other cases the transduction pathways are independent (Batschauer et al 1996).…”

Section: Introductionmentioning

confidence: 99%

Isoforms of chalcone synthase in Daucus carota L. and their differential expression in organs from the European wild carrot and in ultraviolet-A-irradiated cell cultures

Hirner

Seitz

2000

Planta

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Two isoforms of chalcone synthase (CHS) were isolated from cDNA libraries derived from UV-A-irradiated anthocyanin-accumulating (DCb) and non-accumulating (DCs) cell cultures of carrot (Daucus carota L.). The clones designated as DcCHS1, which were present only in the DCb library, had a deduced primary sequence of 389 amino acids and an expected molecular mass of 42.7 kDa, and seem to be alleles of those cloned by Ozeki et al. (1993). The second isoform (DcCHS2) was present in both libraries. It had the highest degree of similarity (97.7%) to parsley CHS over all 397 amino acids. The expected molecular mass of the corresponding protein was 43.6 kDa. Results obtained from Southern blot analysis indicated the existence of at least two CHS genes in carrot. A transient enhancement of the DcCHS1 mRNA level after continuous irradiation with UV-A light could only be observed in anthocyanin-accumulating cultures, whereas an increase in DcCHS2 mRNA was seen in both cell lines. The maximum accumulation of CHS mRNA occurred 48 h after the onset of UV-A irradiation. In the European wild carrot the accumulation of DcCHS1 mRNA was restricted to the red central flowers, whereas the DcCHS2 mRNA was detectable in all red and white petals, as well as leaves, but was absent in stems and roots. The expression of DcCHS1 was restricted to anthocyanin-accumulating cells or organs. The heterologous expression of both cDNAs in Escherichia coli resulted in immunostainable bands of different sizes on the Western blot and high levels of catalytic CHS activity.

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“…1998, Moriguchi et a/. 1999) and a variety of environmental stimuli such as light(Fuglevand et a/. 1996, Glassgen et a/.…”

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confidence: 99%

Transcriptional Control of Chalcone Synthase by Environmental Stimuli

Sakuta

2000

J Plant Res

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Plants exhibit a number of adaptive and protective responses to environmental stimuli. Of particular significance is the induction of the biosynthesis of flavonoids which are well characterized as defense substances ie. UV protectants and antimicrobial compounds phytoalexins. Chalcone synthase (CHS), the key enzyme of flavonoid biosynthesis, is induced by environmental stresses such as UV, mechanical wounding and pathogen attack. Characterization of the cis elements and trans factors involved inCHS expression has enabled delineation of the terminal stage of the signal transduction pathways underlying its induction by environmental stimuli. This review summarizes recent advances in studies of transcriptional activation of CHS following environmental stimuli.

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UV-B, UV-A, and Blue Light Signal Transduction Pathways Interact Synergistically to Regulate Chalcone Synthase Gene Expression in Arabidopsis

Cited by 52 publications

References 0 publications

Characterization and Transcriptional Profiling of Ginkgo biloba Mevalonate Diphosphate Decarboxylase Gene (GbMVD) Promoter Towards Light and Exogenous Hormone Treatments

Characterization and Transcriptional Profiling of Ginkgo biloba Mevalonate Diphosphate Decarboxylase Gene (GbMVD) Promoter Towards Light and Exogenous Hormone Treatments

Isoforms of chalcone synthase in Daucus carota L. and their differential expression in organs from the European wild carrot and in ultraviolet-A-irradiated cell cultures

Transcriptional Control of Chalcone Synthase by Environmental Stimuli

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