Utilization of the biorreactor of imersion by bubbles at the micropropagation of Ananas comosus L. Merril

Abstract

The research for new techniques of in vitro cultivation is being object of many studies around the world, in order to

Bioreactor shoot cultures of Rhododendron tomentosum (Ledum palustre) for a large-scale production of bioactive volatile compounds

“…Moreover, the agitated liquid culture serves as a preparatory step for introducing microshoots to large-scale installations, allowing to investigate the influence of the miscellaneous cultivation factors on biomass accumulation (Grzegorczyk and Wysokinska 2008). According to many reports, the controlled microenvironment, in terms of nutrients and gas transfer in bioreactors, is associated with higher multiplication rate, acceleration of biomass growth and improvement of secondary metabolites productivity (Georgiev et al 2014;Jang et al 2016;Paek et al 2005;Scheidt et al 2009;Yan et al 2011;Zobayed et al 2004). However, it should be noted that so far there are only a few articles concerning essential oil accumulation in bioreactor-grown microshoots (Fulzele et al 1995;Hilton et al 1995;Tisserat and Vaughn 2008).…”

Antidiabetic activities of ethanol extract of dry matters of culture broth of Coriolus versiolor in submerged culture

“…Its medicinal value was recorded in the Compendium of Chinese Materia Medica and has been gaining acceptance among the patients worldwide (Kidd, 2000;Wasser and Weis, 1999). Because the growth of this mushroom need several months and the difficulty in cultivation, so it is generally recognized that growing its mycelia in a defined medium by submerged fermentation could be a rapid and alternative method to obtain the fungal biomass of consistent quality (Lu et al, 2007;Ramadas et al, 2009;Scheidt et al, 2009). Although several studies have focused on the therapeutic effects of the fruit body of the C.versicolor, little information is available about its antihyperglycemic and antilipidperoxidative effects in submerged culture.…”

Biorreator na micropropagação de abacaxi ornamental

“…The benefits of air injection in liquid cultivation systems (TI), as well as gas exchange provided by the use of porous membranes (NVS), are directly related to the multiplication and promotion of in vitro growth of different species, such as orchid (MOREIRA et al, 2013), pineapple (SCHEIDT et al, 2009), grape (JIN et al, 2013) and medicinal plants (PIATCZAK et al, 2014), among others. The benefits of air injection and the promotion of gas exchange in the in vitro multiplication of A. comosus var.…”

“…Cytokinins have an action in several plant development processes, including cell division, differentiation of cell cultures and sprout induction (PIATCZAK et al, 2014). Ananas comosus micropropagation studies have yielded efficient results in sprout induction with cytokinin BAP (SILVA et al, 2007;SCHEIDT et al, 2009;AYENEW et al, 2013).…”