Utilization of Oil Industry Residues for the Production of Rhamnolipids by <i>Pseudomonas indica</i>

Bhardwaj, Garima; Cameotra, Swaranjit Singh; Chopra, Harish Kumar

doi:10.1007/s11743-015-1711-9

Cited by 12 publications

(4 citation statements)

References 24 publications

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“…In addition to the volatility of the solvent, surface tension can also influence the ultrasonication effects, as previously mentioned (Bussemaker and Zhang, 2013). It has been discussed that lower surface tension can result in enhanced ultrasonication effects (Cheng et al, 2010 andBhardwaj et al, 2015). The surface tension of d-limonene at 25°C is 25.8 dyne•cm -1 which is lower than that of p-cymene (28.5 dyne•cm -1 ) (Tanzi et al, 2012).…”

Section: Ultrasonication Resultsmentioning

confidence: 96%

Enhancing Downstream Processing Of Algal-Based Biofuel Using Novel Fused Bacteria And Green Solvents

Fiayaz¹

2021

Preprint

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The present study investigated the utilization of algal biomass to produce bio-oil and acetone, butanol, and ethanol (ABE) products. Novel Clostridia fusants (C. beijernickii + C. thermocellum-CbCt and C. acetobutylicum + C. thermocellocum-CaCt) were developed using protoplast fusion technique and subsequently subjected to UV radiation for strain enhancement. Resultant mutated fusants showed improvement in thermal stability and higher resistance to biobutanol toxicity. Algal biomass was initially subjected to various hydrolysis treatments prior to fermentation. Combination treatment of thermal, chemical, and enzymatic resulted in maximum sugar release of 27.78 g/L. Maximum biobutanol concentration from fermentation using CbCt resulted in 7.98 g/L. Fermentation using CaCt produced a concentration of 7.39 g/L. Oil extraction from virgin algae investigated a green, bio-based approach using terpenes with ultrasonication and a modified, Bligh and Dyer method, separately. Combination method, ultrasonication followed by the modified Bligh and Dyer, resulted in oil yield of 46.27% (dlimonene) and 39.85% (p-cymene). Oil extraction was also produced from an algae sample following fermentation. Combined extraction method using fermentation sample resulted in oil yield of 65.04%.

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Section: Ultrasonication Resultsmentioning

confidence: 96%

Enhancing Downstream Processing Of Algal-Based Biofuel Using Novel Fused Bacteria And Green Solvents

Fiayaz¹

2021

Preprint

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“…Two milliliters of the hydrocarbons (rice bran oil, olive oil, kerosene, n-dodecane, and hexane) were mixed with 2 mL of supernatant (cell-free) in a test tube and mixed vigorously for 2 min in a vortex shaker (LABCO) at high speed. Emulsification activity was calculated by dividing height within the emulsion layer by the height of the whole solution [14].…”

Section: Emulsification Activitymentioning

confidence: 99%

“…To maintain constant flow a vacuum pump was also connected with the column. The column was started with distilled water followed by biosurfactant (200 mg in 10 mL distilled water), and the amounts of kerosene recovered (%) were compared, respectively [14,16].…”

Section: Microbial Enhanced Oil Recovery (Meor)mentioning

confidence: 99%

Biosurfactant from Lysinibacillus chungkukjangi from Rice Bran Oil Sludge and Potential Applications

Bhardwaj

Cameotra

Chopra

2016

J Surfact & Detergents

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A newly discovered bacterium, Lysinibacillus chungkukjangi, was isolated from the sludge of rice bran oil processing. When the bacterium was grown on rice bran, it produced biosurfactant which reduced the surface tension of the media to 27.9 from 72 mN/m. The biosurfactant was recovered by a solvent extraction method and characterized with the help of various structure elucidation techniques viz. FTIR, 1H‐ and 13C‐NMR spectroscopy and LC–MS analysis. The combined results of FTIR and NMR revealed the presence of carbonyl, olefinic and aliphatic groups, with the typical spectra of lipids. Moreover, LC–MS analysis also supported the same information. The biosurfactant was also studied for its anti‐oxidant and microbial enhanced oil recovery (MEOR) potential. The anti‐oxidant activity was observed by the DPPH free radical scavenging method using ascorbic acid as the standard. The IC50 (the half maximal inhibitory concentration) was calculated and for standard, it was 0.056 mg/mL and for the biosurfactant it turned out to be 1.3 mg/mL which shows its good anti‐oxidant potential. The sandpack test was performed to check its MEOR potential and kerosene was recovered up to 90 %, which shows its excellent applicability in the MEOR processes.

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“…Among the various alternative ways to reduce the RBS production cost such as strain improvement, optimization of production media, and so on, the use of waste products as raw materials is of paramount importance, as these waste products account for 10-30% of the final production cost along with the reduction of expenses required for the treatment of the wastes (Banat et al, 2014;Cameotra and Makkar, 1998). Various renewable substrates of different sources including waste products have been successful (Makkar and Cameotra, 1999), ethanol and vegetable oil (Matsufuju et al, 1997), oil industry residues (Bharadwaj et al, 2015), rice bran (Bharadwaj et al, 2016), orange fruit peel (George and Jayachandran, 2008), paneer whey (Patowary et al, 2016a), and so on are some of the examples. However, to date, no one has reported the use of food wastes like bakery waste (BW) for cost-effective production of RBS.…”

Section: Introductionmentioning

confidence: 99%

Recycling of Bakery Waste as an Alternative Carbon Source for Rhamnolipid Biosurfactant Production

Patowary

Das

Patowary

et al. 2018

J Surfact & Detergents

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Production of a rhamnolipid biosurfactant (RBS) using discarded mixed bakery waste (BW) employing bacterial strain Pseudomonas aeruginosa strain PG1 (identified by 16 s rDNA sequencing) was investigated for bioconversion of the food waste. Dry and powder form BW was supplemented with mineral salt media (MSM) as a sole carbon source for production of RBS. RBS production was measured based on the drop collapse assay and surface tension (ST) reduction of the culture media. Production of RBS in the culture media was enhanced by optimizing the carbon source (BW) concentration and the proper nitrogen source along with the pH of the MSM. Under optimized culture conditions, 11.56 g L −1 day −1 crude biosurfactant (BS) was achieved. The RBS had the ability to reduce the ST of the optimized MSM from 72.0 to 25.8 mN m −1 during culture, where the critical micelle concentration (CMC) of the biosurfactant was found to be 100 mg L −1 . Liquid Chromatography Mass Spectroscopy (LC-MS), Fourier Transform Infrared spectroscopy (FTIR), and scanning electron microscopy (SEM)-energy dispersive X-ray spectroscopy (EDS) analyses of the purified BS confirmed that it is of rhamnolipid in nature and it is made up of both monorhamnolipid and dirhamnolipid congeners. Furthermore, the RBS did not express any cytotoxic effect on the cell line of mouse L292 fibroblastic cell indicating the biosafety nature of the high-value biomolecule.

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Utilization of Oil Industry Residues for the Production of Rhamnolipids by Pseudomonas indica

Cited by 12 publications

References 24 publications

Enhancing Downstream Processing Of Algal-Based Biofuel Using Novel Fused Bacteria And Green Solvents

Enhancing Downstream Processing Of Algal-Based Biofuel Using Novel Fused Bacteria And Green Solvents

Biosurfactant from Lysinibacillus chungkukjangi from Rice Bran Oil Sludge and Potential Applications

Recycling of Bakery Waste as an Alternative Carbon Source for Rhamnolipid Biosurfactant Production

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